Design and Evaluation of Next Generation Albumin-thioredoxin Fusion Protein for Multiple Organ Failure Treatment

• Project/Area Number: 24390043
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Medical pharmacy
• Research Institution: Sojo University
• Principal Investigator: OTAGIRI Masaki 崇城大学, 薬学部, 教授 (80120145)
• Co-Investigator(Kenkyū-buntansha): MARUYAMA Toru 熊本大学, 薬学部, 教授 (90423657) ; WATANABE Hiroshi 熊本大学, 薬学部, 准教授 (70398220)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥18,850,000 (Direct Cost: ¥14,500,000、Indirect Cost: ¥4,350,000); Fiscal Year 2014: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2013: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000); Fiscal Year 2012: ¥9,880,000 (Direct Cost: ¥7,600,000、Indirect Cost: ¥2,280,000)
• Keywords: アルブミン / ナノメディスン / レドックス / 多臓器不全治療 / 病態モデル / 肺障害 / 肝障害 / 腎障害 / チオレドキン

Outline of Final Research Achievements

This research investigated the therapeutic effect of albumin-thioredoxin (HSA-Trx) on multiple organ failure model using mouse models of primary organ failure such as lungs, liver and kidney.
1) HSA-Trx was found to produce effective therapeutic effect in Bleomycin induced pulmonary fibrosis. 2) The inhibitory effect of fusion protein on acetaminophen induced liver disorder was found to occur in a concentration dependent manner. 3) Effectiveness of the fusion protein in cisplatin nephropathy was evaluated. The fusion protein was found to inhibit increases in SCr, BUN and NAG activity, as well as reduction in CLcr.

Research Products

• [Journal Article] Cross-linked human serum albumin dimer has the potential for use as a plasma retaining-agent for the fatty acid-conjugated antidiabetic drugs. (2015)
  • Author(s): Taguchi K, Chuang VTG, Yamasaki K, Urata Y, Tanaka R, Anraku M, Seo H, Kawai K, Maruyama T, Komatsu T, Otagiri M
  • Journal Title: J Pharm Pharmacol. Volume: 67 Issue: 2 Pages: 255-263
  • DOI: 10.1111/jphp.12338
  • Peer Reviewed
• [Journal Article] Polythiol-containing, recombinant mannosylated-albumin is a superior CD68+/CD206+ Kupffer cell-targeted nano-antioxidant for the treatment of two acute hepatitis models. (2015)
  • Author(s): Maeda H, Hirata K, Watanabe H, Ishima Y, Chuang VT, Taguchi K, Inatsu A, Kinoshita M, Tanaka M, Sasaki Y, Otagiri M, Maruyama T.
  • Journal Title: J Pharmacol Exp Ther Volume: 352 Issue: 2 Pages: 244-257
  • DOI: 10.1124/jpet.114.219493
  • Peer Reviewed / Open Access
• [Journal Article] Synthesis and therapeutic effect of styrene-maleic acid copolymer-conjugated pirarubicin. (2015)
  • Author(s): Tsukigawa K, Liao L, Nakamura H, Fang J, Greish K, Otagiri M, Maeda H.
  • Journal Title: Cancer Sci. Volume: 106 Issue: 3 Pages: 270-278
  • DOI: 10.1111/cas.12592
  • Peer Reviewed
• [Journal Article] Poly-S-nitrosated human albumin enhances the antitumor and antimetastasis effect of bevacizumab, partly by inhibiting autophagy through the generation of nitric oxide. (2015)
  • Author(s): Ishima Y, Inoue A, Fang J, Kinoshita R, Ikeda M, Watanabe H, Maeda H, Otagiri M, Maruyama T.
  • Journal Title: Cancer Sci. Volume: 106 Issue: 2 Pages: 194-200
  • DOI: 10.1111/cas.12577
  • Peer Reviewed / Open Access
• [Journal Article] Interactions of Linezolid with Two Major Serum Proteins, Human Serum Albumin and Alpha-1 Acid Glycoprotein. (2015)
  • Author(s): Taguchi K, Yamasaki1 K, Shiga A, Otagiri M, Seo H.
  • Journal Title: J. Pharmacol Clin Toxicol. Volume: 3 Pages: 1045-1045
  • Peer Reviewed
• [Journal Article] N-acetyl-Lmethionine is a superior protectant of human serum albumin against photooxidation and reactive oxygen species compared to N-acetyl-L-tryptophan (2014)
  • Author(s): Kouno Y, Anraku M, Yamasaki K, Okayama Y, Iohara D, Ishima Y, Maruyama T, Kragh-Hansen U, Hirayama F, Otagiri M
  • Journal Title: Biochim Biophys Acta Volume: (in press) Issue: 9 Pages: 2806-2812
  • DOI: 10.1016/j.bbagen.2014.04.014
  • Peer Reviewed
• [Journal Article] Carbon monoxide-bound hemoglobin-vesicles for the treatment of bleomycin-induced pulmonary fibrosis. (2014)
  • Author(s): Nagao S, Taguchi K, Sakai H, Tanaka R, Horinouchi H, Watanabe H, Kobayashi K, Otagiri M, Maruyama T.
  • Journal Title: Biomaterials. Volume: 35 Issue: 24 Pages: 6553-6562
  • DOI: 10.1016/j.biomaterials.2014.04.049
  • Peer Reviewed
• [Journal Article] Tuning of Poly-S-Nitrosated Human Serum Albumin as Superior Antitumor Nanomedicine (2014)
  • Author(s): Ishima Y, Fang J, Kragh-Hansen U, Yin H, Liao L, Katayama N, Watanabe H, Kai T, Suenaga A, Maeda H, Otagiri M, Maruyama T
  • Journal Title: J Pharm Sci Volume: 103 Issue: 7 Pages: 2184-2188
  • DOI: 10.1002/jps.24020
  • Peer Reviewed
• [Journal Article] Effects of chitosan on oxidative stress and related factors in hemodialysis patients. (2014)
  • Author(s): Anraku M, Tanaka M, Hiraga A, Nagumo K, Imafuku T, Maezaki Y, Iohara D, Uekama K, Watanabe H, Hirayama F, Maruyama T, Otagiri M
  • Journal Title: Carbohydr Polym Volume: 112 Pages: 152-157
  • DOI: 10.1016/j.carbpol.2014.05.078
  • Peer Reviewed
• [Journal Article] Therapeutic impact of erythropoietin-encapsulated liposomes targeted to bone marrow on renal anemia. (2014)
  • Author(s): Miyazaki Y, Taguchi K, Sou K, Watanabe H, Ishima Y, Miyakawa T, Mitsuya H, Fukagawa M, Otagiri M, Maruyama T.
  • Journal Title: Mol Pharm. Volume: 11 Issue: 11 Pages: 4238-4248
  • DOI: 10.1021/mp500453a
  • Peer Reviewed
• [Journal Article] Therapeutic Impact of Human Serum Albumin-Thioredoxin Fusion Protein on Influenza Virus-Induced Lung Injury Mice. (2014)
  • Author(s): Tanaka R, Ishima Y, Enoki Y, Kimachi K, Shirai T, Watanabe H, Chuang VT, Maruyama T, Otagiri M.
  • Journal Title: Front Immunol Volume: 5 Pages: 561-561
  • DOI: 10.3389/fimmu.2014.00561
  • Peer Reviewed / Open Access
• [Journal Article] Albumin Fusion Prolongs the Antioxidant and Anti-Inflammatory Activities of Thioredoxin in Mice with Acetaminophen-Induced Hepatitis. (2014)
  • Author(s): Tanaka R, Ishima Y, Maeda H, Kodama A, Nagao S, Watanabe H, Chuang VT, Otagiri M, Maruyama T.
  • Journal Title: Mol Pharm. Volume: 印刷中
  • Peer Reviewed
• [Journal Article] Albumin fusion renders thioredoxin an effective anti-oxidative and anti-inflammatory agent for preventing cisplatin-induced nephrotoxicity. (2014)
  • Author(s): Kodama A, Watanabe H, Tanaka R, Kondo M, Chuang VT, Wu Q, Endo M, Ishima Y, Fukagawa M, Otagiri M, Maruyama T.
  • Journal Title: Biochim Biophys Acta. Volume: 1840 Issue: 3 Pages: 1152-1162
  • DOI: 10.1016/j.bbagen.2013.12.007
  • Peer Reviewed
• [Journal Article] Poly-s-nitrosated albumin as a safe and effective multifunctional antitumor agent : characterization, biochemistry and possible future therapeutic applications (2013)
  • Author(s): Ishima Y, Kragh-Hansen U, Maruyama T, Otagiri M
  • Journal Title: Biomed Res Int Volume: 2013 Pages: 353892-353892
  • DOI: 10.1155/2013/353892
  • Peer Reviewed
• [Journal Article] Effect of albumin on transthyretin and amyloidogenic transthyretin Val30Met disposition and tissue deposition in familial amyloidotic polyneuropathy. (2013)
  • Author(s): Taguchi K, Jono H, Kugimiya-Taguchi T, Nagao S, Su Y, Yamasaki K, Mizuguchi M, Maruyama T, Ando Y, Otagiri M.
  • Journal Title: Life Sci. Volume: 18 Issue: 25-26 Pages: 1017-22
  • DOI: 10.1016/j.lfs.2013.10.031
  • Peer Reviewed
• [Journal Article] Albumin-drug interaction and its clinical implication. (2013)
  • Author(s): Yamasaki K, Chuang VT, Maruyama T, Otagiri M.
  • Journal Title: Biochim Biophys Acta. Volume: 1830 Issue: 12 Pages: 5435-43
  • DOI: 10.1016/j.bbagen.2013.05.005
  • Peer Reviewed
• [Journal Article] Redox properties of serum albumin. (2013)
  • Author(s): Anraku M, Chuang VT, Maruyama T, Otagiri M.
  • Journal Title: Biochim Biophys Acta. Volume: 1830 Issue: 12 Pages: 5465-5472
  • DOI: 10.1016/j.bbagen.2013.04.036
  • Peer Reviewed
• [Journal Article] UW solution improved with high anti-apoptotic activity by S-nitrosated human serum albumin. (2013)
  • Author(s): Ishima Y
  • Journal Title: Nitric Oxide. Volume: 印刷中 Pages: 36-42
  • DOI: 10.1016/j.niox.2013.01.004
  • Peer Reviewed
• [Journal Article] Albumin domain II mutant with high bilirubin binding affinity has a great potential as serum bilirubin excretion enhancer for hyperbilirubinemia treatment. (2013)
  • Author(s): Minomo A
  • Journal Title: Biochim Biophys Acta. Volume: 印刷中 Issue: 4 Pages: 141-8
  • DOI: 10.1016/j.bbagen.2013.01.006
  • Peer Reviewed
• [Journal Article] Interaction between two sulfate-conjugated uremic toxins, p-cresyl sulfate and indoxyl sulfate, during binding with human serum albumin. (2013)
  • Author(s): Watanabe H
  • Journal Title: Biochim Biophys Acta. Volume: 40 Issue: 7 Pages: 1423-8
  • DOI: 10.1124/dmd.112.045617
  • Peer Reviewed
• [Journal Article] Carbon Monoxide Bound Red Blood Cells Protect Red Blood Cell Transfusion-induced Hepatic Cytochrome P450 Impairment in Hemorrhagic-shock Rats. Drug Metab Dispos. (2013)
  • Author(s): Ogaki S, Taguchi K, Watanabe H, Otagiri M, and Maruyama T.
  • Journal Title: Drug Metab Dispos. Volume: 41 Issue: 1 Pages: 141-8
  • DOI: 10.1124/dmd.112.048744
  • Peer Reviewed
• [Journal Article] S-nitrosylated a-l-acid glycoprotein kills drug-resistant bacteria and aids survival in sepsis (2013)
  • Author(s): Watanabe K, Ishima Y, Akaike T, Sawa T, Kuroda T, Ogawa W, Watanabe H, Suenaga A, Kai T, Otagiri M, and Maruyama T.
  • Journal Title: FASEB J Volume: 27 Issue: 1 Pages: 391-398
  • DOI: 10.1096/fj.12-217794
  • Peer Reviewed
• [Journal Article] Long-acting human serum albumin-thioredoxin fusion protein suppresses bleomycin-induced pulmonary fibrosis progression. (2013)
  • Author(s): Tanaka R, Watanabe H, Kodama A, Chuang VT, Ishima Y, Hamasaki K, Tanaka K, Mizushima T, Otagiri M, Maruyama T.
  • Journal Title: J Pharmacol Exp Ther. Volume: 345 Issue: 2 Pages: 271-283
  • DOI: 10.1124/jpet.112.201814
  • Peer Reviewed
• [Journal Article] A human serum albumin-thioredoxin fusion protein prevents experimental contrast-induced nephropathy. (2013)
  • Author(s): Kodama A
  • Journal Title: Kidney Int. Volume: 印刷中 Issue: 3 Pages: 446-54
  • DOI: 10.1038/ki.2012.429
  • Peer Reviewed
• [Journal Article] Quantitative evaluation of the role of cysteine and methionine residues in the antioxidant activity of human serum albumin using recombinant mutants (2012)
  • Author(s): Iwao Y
  • Journal Title: IUBMB Life Volume: 64 Issue: 5 Pages: 450-454
  • DOI: 10.1002/iub.567
  • Peer Reviewed
• [Journal Article] Arginine 485 of human serum albumin interacts with the benzophenone moiety of ketoprofen in the binding pocket of subdomain in A and Ill B. (2012)
  • Author(s): Kaneko K, Chuang VT, Ito T, Suenaga A, Watanabe H, Maruyama T, Otagiri M.Pharmazie.
  • Journal Title: Pharmazie. Volume: 67 Pages: 414-418
  • Peer Reviewed
• [Journal Article] α1-acid glycoprotein up-regulates CD163 via TLR4/CD14 pathway: possible protection against hemolysis-induced oxidative stress. (2012)
  • Author(s): Komori H, Watanabe H, Shuto T, Kodama A, Maeda H, Watanabe K, Kai H, Otagiri M, Maruyama T.
  • Journal Title: J Biol Chem. Volume: 287 Issue: 36 Pages: 30688-700
  • DOI: 10.1074/jbc.m112.353771
  • Peer Reviewed
• [Journal Article] Pharmaceutical aspects of the recombinant human serum albumin dimer: Structural characteristics, biological properties, and medical applications. (2012)
  • Author(s): Taguchi K, Giam Chuang VT, Maruyama T, Otagiri M. 2012:
  • Journal Title: J Pharm Sci. Volume: 101 Pages: 3033-3046
  • Peer Reviewed
• [Journal Article] S-Guanylation of human serum albumin is a unique posttranslational modification and results in a novel class of antibacterial agents (2012)
  • Author(s): Ishima Y, Hoshino H, Shinagawa T, Watanabe K, Akaike T, Sawa T, Kragh-Hansen U, Kai T, Watanabe H, Maruyama T, and Otagiri M
  • Journal Title: J. Pharm. Sci Volume: 101 Issue: 9 Pages: 3222-3229
  • DOI: 10.1002/jps.23143
  • Peer Reviewed
• [Journal Article] A fourteen-day observation and pharmacokinetic evaluation after a massive intravenous infusion of hemoglobin-vesicles (artificial oxygen carriers) in cynomolgus monkeys. (2012)
  • Author(s): Taguchi K, Watanabe H, Sakai H, Horinouchi H, Kobayashi K, Maruyama T, and Otagiri M
  • Journal Title: J Drug Metab Toxicol. Volume: 3 Issue: 4 Pages: 128-34
  • DOI: 10.4172/2157-7609.1000128
  • Peer Reviewed
• [Journal Article] Liposome-encapsulated hemoglobin (hemoglobin-vesicle) is not transferred from mother to fetus at the late stage of pregnancy ill the rat model. (2012)
  • Author(s): Kaga M, Li H, Ohta H, Taguchi K, Ogaki S, Izumi H, Inagaki M, Tsuchiya S, Okamura K, 0tagiri M, Sakai H, and Yaegashi N
  • Journal Title: Life Sci. Volume: 91 Pages: 420-428
  • Peer Reviewed
• [Journal Article] A uremic toxin, 3-carboxy-4-methyl-5-propyl-2-furanpropionate induces cell damage to proximal tubular cells via the generation of a radical intermediate. (2012)
  • Author(s): Yohei Miyamoto, Yasunori Katsumi Mera, Hiroshi Watanabe, Daisuke Kadowaki, Yu Ishima, Victor Tuan Giam Chuang, Keizo Sato, Masaki Otagiri, Toru Maruyama.
  • Journal Title: Biochemical Pharmacology Volume: 84 Issue: 9 Pages: 1207-1214
  • DOI: 10.1016/j.bcp.2012.07.033
  • Peer Reviewed
• [Journal Article] Elucidation of the therapeutic enhancer mechanism of poly-S-nitrosated human serum albumin against multidrug-resistant tumor in animal models. (2012)
  • Author(s): Ishima Y
  • Journal Title: J Control Release. Volume: 164 Issue: 1 Pages: 1-7
  • DOI: 10.1016/j.jconrel.2012.10.003
  • Peer Reviewed
• [Journal Article] Effect of acetaminophen on progression of renal damage in adenine induced renal failure model rat. (2012)
  • Author(s): Kadowaki D, Sumikawa S, Arimizu K, Taguchi K, Kitamura K, Ishitsuka Y, Narita Y, Irie T, Maruyama T, Otagiri M, Hirata S
  • Journal Title: Life Sci. Volume: 91 Issue: 25-26 Pages: 1304-1308
  • DOI: 10.1016/j.lfs.2012.09.018
  • Peer Reviewed
• [Journal Article] Characterization of the hepatic cellular uptake of α_1-acid glycoprotein (AGP), Part 2 : Involvement of the hemoglobin β-chain on plasma membranes in the uptake of human AGP by liver parenchymal cells (2011)
  • Author(s): 小森久和
  • Journal Title: Journal of Pharmaceutical Sciences Volume: 101 Issue: 4 Pages: 1607-1615
  • DOI: 10.1002/jps.22804
  • Peer Reviewed
• [Journal Article] Characterization of the hepatic cellular uptake of α_1-acid glycoprotein (AGP), Part 1 : A peptide moiety of human AGP is recognized by the hemoglobin β-chain on mouse liver parenchymal cells (2011)
  • Author(s): 西弘二、小森久和
  • Journal Title: Journal of Pharmaceutical Sciences Volume: 101 Issue: 4 Pages: 1599-1606
  • DOI: 10.1002/jps.23015
  • Peer Reviewed
• [Presentation] S-ニトロソ化α1-酸性タンパク質による抗生物質の多剤耐性克服と機序解明 (2014)
  • Author(s): 丸山 徹
  • Organizer: 第36回 生体膜と薬物の相互作用シンポジウム
  • Place of Presentation: 徳島大学 大塚講堂（徳島県徳島市）ほか
  • Year and Date: 2014-11-20 – 2014-11-21
• [Presentation] Therapeutic Applications of Albumin-Thioredoxin Fusion Protein. (2014)
  • Author(s): Masaki Otagiri
  • Organizer: 10th European Symposium on Biochemical Engineering Sciences and 6th International Forum on Industrial Bioprocesses Lille
  • Place of Presentation: Lille (France)
  • Year and Date: 2014-09-07 – 2014-09-10
  • Invited
• [Presentation] Pharmacokinetics of serum albumin variants. (2014)
  • Author(s): M.Otagiri
  • Organizer: Innovation workshop on Albumin: The next generation protein therapeutics 2014
  • Place of Presentation: Chicago (USA)
  • Year and Date: 2014-07-12
  • Invited
• [Presentation] NO付加型ヒト血清アルブミンの臨床応用へのアプローチ (2014)
  • Author(s): 異島 優
  • Organizer: 第14回日本NO学会学術集会
  • Place of Presentation: ホテルニューオータニ佐賀（佐賀県佐賀市）
  • Year and Date: 2014-05-16 – 2014-05-17
• [Presentation] インフルエンザ肺炎モデルマウスに対するヒト血清アルブミン－チオレドキシン融合タンパク質の有効性評価 (2014)
  • Author(s): 異島優
  • Organizer: 日本薬学会第134年会
  • Place of Presentation: 熊本・ホテル日航熊本他
• [Presentation] エリスロポェチン骨髄デリバリーシステムの構築と腎性貧血治療への応用 (2013)
  • Author(s): 丸山徹
  • Organizer: 日本薬学会第133年会
  • Place of Presentation: パシフィコ横浜(神奈川県)
  • Year and Date: 2013-03-28
• [Presentation] S-ニトロソ化により獲得するa1-酸性等タンパク質(AGP)の抗菌活性 (2013)
  • Author(s): 異島優
  • Organizer: 日本薬学会第133年会
  • Place of Presentation: パシフィコ横浜(神奈川県)
  • Year and Date: 2013-03-28
• [Presentation] ブレオマイシン誘発肺線維症に対するアルブミン-チオレドキシン融合タンパク質の有用性評価 (2013)
  • Author(s): 小田切優樹
  • Organizer: 日本薬学会第133年会
  • Place of Presentation: パシフィコ横浜(神奈川県)
  • Year and Date: 2013-03-28
• [Presentation] Development of recombinant serum (2013)
  • Author(s): Masaki Otagiri
  • Organizer: The International Liver Congress 2013 48th annual meeting of the European association for the study of the liver
  • Place of Presentation: オランダ・アムステルダム
  • Invited
• [Presentation] Albumin-Thioredoxin Fusion Protein is Effective in Suppressing Lung Injury (2013)
  • Author(s): Masaki Otagiri
  • Organizer: 9th Retrometabolism Based Drug Design and Targeting Conferenece
  • Place of Presentation: アメリカ・オーランド
  • Invited
• [Presentation] Poly-S-nitrosated Human Serum Albumin inhibits the Expression of P-glycoprotein Transporter in Human Multidrug-resistant Tumo (2013)
  • Author(s): Yu Ishima
  • Organizer: the 5th Asian Arden conference
  • Place of Presentation: 愛知・Aichi Gakuin University
• [Presentation] PRECLINICAL STUDIES OF HEMOGLOBIN-VESICLES AS AN ARTIFICIAL OXYGEN CARRIER IN NON-HUMAN PRIMATE (2013)
  • Author(s): Kazuaki Taguchi
  • Organizer: 日本薬物動態学会 第28回年会
  • Place of Presentation: 東京・タワーホール船堀
• [Presentation] THERAPEUTIC IMPACT OF HUMAN SERUM ALBUMIN-THIOREDOXIN FUSION PROTEIN, LONG-ACTING ANTI-OXIDATIVE AND ANTI-INFLAMMATORY MODULATOR, AGAINST ACETAMINOPHEN-INDUCED ACUTE LIVER FAILURE (2013)
  • Author(s): Ryota Tanaka
  • Organizer: 日本薬物動態学会 第28回年会
  • Place of Presentation: 東京・タワーホール船堀
• [Presentation] Preclinical studies of hemoglobin-vesicles as an artificial oxygen carrier in non-human primate (2013)
  • Author(s): Kazuaki Taguchi
  • Organizer: The XIVth International Symposium on Blood Substitutes and Oxygen Therapeutics
  • Place of Presentation: 中国・成都
• [Presentation] The effect of N-acetyl-methionine on photo-stability of albumin products (2013)
  • Author(s): Makoto Anraku
  • Organizer: Asian Federation for Pharmaceutical Sciences 2013
  • Place of Presentation: 韓国・済州島
• [Presentation] S-ニトロソ化アルブミンタイマーはEPR効果を増強する (2012)
  • Author(s): 井上亜希
  • Organizer: 日本薬剤学会第27年会
  • Place of Presentation: 神戸国際会議場(兵庫県)
• [Presentation] アルギニンペプチドを利用した細胞膜透過型アルブミンの設計と評価 (2012)
  • Author(s): 濱崎慶輔
  • Organizer: 日本薬剤学会第27年会
  • Place of Presentation: 神戸国際会議場(兵庫県)
• [Presentation] In vivoファージディスプレイ法を用いた新規腎送達ペプチドの探索 (2012)
  • Author(s): 弥永直樹
  • Organizer: 日本薬剤学会第27年会
  • Place of Presentation: 神戸国際会議場(兵庫県)
• [Presentation] S-ニトロソ化に伴うα1-酸性糖タンパク質(AGP)の抗菌機能獲得と感染症治療への応用 (2012)
  • Author(s): 渡辺佳織
  • Organizer: 第12回日本NO学会学術集会
  • Place of Presentation: 神戸国際会議場(兵庫県)
• [Presentation] S-ニトロソ化アルブミンにより抗アポトーシス効果を付与した改良型臓器保存液の開発 (2012)
  • Author(s): 異島優
  • Organizer: 第12回日本NO学会学術集会
  • Place of Presentation: 神戸国際会議場(兵庫県)
• [Presentation] ヒト血清アルブミンドメインIIによるビリルビン尿中排泄促進作用 (2012)
  • Author(s): 小田切優樹
  • Organizer: 第28回日本DDS学会学術集会
  • Place of Presentation: 札幌コンベンションセンター(北海道)
• [Presentation] 出血性ショックモデルラットにおける一酸化炭素付加型赤血球の肝地とクロームP450保護効果 (2012)
  • Author(s): 大柿滋
  • Organizer: 第19回日本血液代替物学会年次大会
  • Place of Presentation: 旭川大雪クリスタルホール(北海道)
• [Presentation] ESI-TOF/MSを用いたシステイン付加型ヒト血清アルブミンの検出と機能相関-慢性肝疾患の影響- (2012)
  • Author(s): 南雲恒平
  • Organizer: 第19回日本血液代替物学会年次大会
  • Place of Presentation: 旭川大雪クリスタルホール(北海道)
• [Presentation] 細胞型人工酸素運搬体 ヘモグロビン小胞体のカニクイザルへの大量投与の結果からみた実効性 (2012)
  • Author(s): 田口和明
  • Organizer: 第19回日本血液代替物学会年次大会
  • Place of Presentation: 旭川大雪クリスタルホール(北海道)
• [Presentation] 血小板代替物 H12 (ADP)リボソームの体内動態に及ぼす血小板減少症の影響 (2012)
  • Author(s): 丸山徹
  • Organizer: 第19回日本血液代替物学会年次大会
  • Place of Presentation: 旭川大雪クリスタルホール(北海道)
• [Presentation] ラット妊娠母体におけるヘモグロビン小胞体の胎盤通過性 (2012)
  • Author(s): 太田英伸
  • Organizer: 第19回日本血液代替物学会年次大会
  • Place of Presentation: 旭川大雪クリスタルホール(北海道)
• [Presentation] S・ニトロソ化に伴うα1・酸性糖タンパク質(AGP)の抗菌機能獲得と感染症治療への応用(第34回生体膜と薬物の相互作用シンポジウム (2012)
  • Author(s): 渡辺佳織
  • Organizer: 第34回生体膜と薬物の相互作用シンポジウム
  • Place of Presentation: 京都大学薬学部(京都府)
• [Presentation] 尿毒症物質p・クレジル硫酸のレドックス特性と腎障害、心血管疾患発症機序解門 (2012)
  • Author(s): 宮本洋平
  • Organizer: 第34回生体膜と薬物の相互作用シンポジウム
  • Place of Presentation: 京都大学薬学部(京都府)
• [Presentation] 一酸化炭素付加赤血球によるクッパー細胞の不活化は輸血誘発肝チトクロームP450機能障害を保護する (2012)
  • Author(s): 大柿滋
  • Organizer: 第34回生体膜と薬物の相互作用シンポジウム
  • Place of Presentation: 京都大学薬学部(京都府)
• [Presentation] p-Cresyl sulfate causes renal tubular cell damage by kmiducing oxidative stress through the activation of NADPH oxidase. (2012)
  • Author(s): Watanabe H
  • Organizer: 49th ERA-EDTA Congress, Paris 2012.
  • Place of Presentation: Paris (France)
• [Presentation] A uremic toxin, 3-carboxy-4-methyl-5- propyl-2-furanpropionate accumulates in proximal tubular cells and induces cell damage through increasing oxidative stress. (2012)
  • Author(s): Miyamoto Y
  • Organizer: 49th ERA-EDTA Congress, Paris 2012.
  • Place of Presentation: Paris (France)
• [Presentation] Human serum albumin as a nanomedecine carrir. (2012)
  • Author(s): OtagiriM
  • Organizer: Nanoformulation 2012.
  • Place of Presentation: Fira Palace, Barcelona(招待講演)
• [Presentation] The potential of albumin for use as a nanomedicine carrier. (2012)
  • Author(s): Otagiri M.
  • Organizer: Montreal 2012 international forum on enviroment and medicine
  • Place of Presentation: Montreal, Quebec, Canada(招待講演)
• [Book] 薬剤学実験法必携マニュアル Ⅱ生物薬剤学 4.薬物分布実験 タンパク結合：血漿・組織タンパク結合 (2014)
  • Author(s): 小田切 優樹、 丸山 徹
  • Total Pages: 423
  • Publisher: 南江堂
• [Book] ACUTE PHASE PROTEINS (2013)
  • Author(s): MasakiOtagiri
  • Total Pages: 180
  • Publisher: INTECH
• [Book] HUMAN SERUM ALBUMIN (2013)
  • Author(s): MasakiOtagiri
  • Total Pages: 510
  • Publisher: Sojo University Publishing Center