|Budget Amount *help
¥14,690,000 (Direct Cost: ¥11,300,000、Indirect Cost: ¥3,390,000)
Fiscal Year 2015: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2014: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2013: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2012: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
|Outline of Final Research Achievements
Gene functions exert by serial actions of transcription and translation, resulting functional proteins. Recently, it is known that non-coding RNAs are very important for these processes. As an example, RNA interference (RNAi) often suppresses gene expression and regulates chromatin structures. In Caenorhabditis elegans, in which RNAi was found for the first time, a phenomenon systemic RNAi has been known: effects of RNAi transmits from one cell to others and the whole body. This phenomenon is also known to exert in mammalian bodies. We used C. elegans to search for genes defective in systemic RNAi and found that rsd-3 is required for the spread of RNAi between cells. rsd-3 was described to be required for systemic RNAi in germ cells but not in somatic cells. We found that rsd-3 is required for both germ cells and somatic cells. rsd-3 was expressed ubiquitously and localized to endosomes and trans Golgi network. We suggest that rsd-3 acts for systemic RNAi via vesicular transport.