| Budget Amount *help |
¥18,720,000 (Direct Cost: ¥14,400,000、Indirect Cost: ¥4,320,000)
Fiscal Year 2014: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2013: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000)
Fiscal Year 2012: ¥7,020,000 (Direct Cost: ¥5,400,000、Indirect Cost: ¥1,620,000)
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| Outline of Final Research Achievements |
We examined the mechanism of palindrome-mediated t(11;22) translocation using plasmid-based assay. Even in the plasmid lacking the replication origin showed translocation when transfected into the human cell line, suggesting that the t(11;22) translocation mechanism is independent of the DNA replication-based mechanism such as FoSTeS. On the other hand, we also examined breakpoints of three non-palindrome gross chromosomal rearrangement. One interstitial deletion and one duplication showed microhomology at the junction, suggesting the rearrangement took place via FoSTeS. In the third deletion case, we identified 11-13 nucleotide sequence at the upstream breakpoint that was repeated four times at the deletion junction. The backward replication slippage or serial replication slippage is the predicted mechanism. These data suggests that the aberrant DNA replication is involved in the generation of a major subset of deletion/duplication.
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