| Budget Amount *help |
¥18,720,000 (Direct Cost: ¥14,400,000、Indirect Cost: ¥4,320,000)
Fiscal Year 2014: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2013: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2012: ¥7,930,000 (Direct Cost: ¥6,100,000、Indirect Cost: ¥1,830,000)
|
|---|
| Outline of Final Research Achievements |
Germ cells are specialized cell types that produce next generations. Germ cells can be reprogrammed into the ES cell-like states, indicating that the cells retain pluripotency. In this study, we developed the in vitro germ cell differentiation system and explored the mechanisms how germ cells are reprogrammed into pluripotency. First, given the fact that germ cells differentiate with mesodermal cells in vivo, we succeeded to generate the fetal germ cell-like cells in vitro by using mesodermal differentiation culture system. Second, we analyzed the functions of signaling pathways and the effects of small molecule compounds in reprogramming of germ cells into ES cell-like cells and in reprogramming of somatic cells into iPS cells. Our results show that germ and somatic cells share the common molecular mechanisms in reprogramming toward pluripotency.
|
