Rhoptry proteins are involved in sporozoite invasion of the mosquito salivary gland
| Project/Area Number |
24390101
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Parasitology (including Sanitary zoology)
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| Research Institution | Ehime University |
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Principal Investigator |
TORII MOTOMI 愛媛大学, プロテオサイエンスセンター, 教授 (20164072)
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| Co-Investigator(Kenkyū-buntansha) |
ISHINO Tomoko 愛媛大学, プロテオサイエンスセンター, 准教授 (40402680)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥18,720,000 (Direct Cost: ¥14,400,000、Indirect Cost: ¥4,320,000)
Fiscal Year 2014: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Fiscal Year 2013: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Fiscal Year 2012: ¥7,020,000 (Direct Cost: ¥5,400,000、Indirect Cost: ¥1,620,000)
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| Keywords | 寄生虫 / マラリア / 感染 / 侵入 / スポロゾイト / 媒介蚊 / 細胞侵入 / 感染動態 / 寄生虫学 / 唾液腺 / ロプトリー / マラリア原虫 / 唾液腺侵入 |
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| Outline of Final Research Achievements |
In this project, we intended to elucidate the function of secretory proteins localized to sporozoite rhoptries during the host invasion process. We identified that 8 secretory proteins are expressed in sporozoites and localized to rhoptries by immuno-electron microscopic analysis. Since most of rhoptry proteins have been believed to be essential for blood stage parasite proliferation, we generated “sporozoite stage-specific gene silencing parasites” by promoter swapping technique in order to analyze their functions during sporozoite development and/ or invasion of the mosquito salivary glands. As a result, we demonstrated that 5 rhoptry proteins play an important role in the mosquito salivary gland invasion by sporozoites.
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Report
(4 results)
Research Products
(11 results)
