| Budget Amount *help |
¥18,720,000 (Direct Cost: ¥14,400,000、Indirect Cost: ¥4,320,000)
Fiscal Year 2014: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Fiscal Year 2013: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Fiscal Year 2012: ¥7,020,000 (Direct Cost: ¥5,400,000、Indirect Cost: ¥1,620,000)
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| Outline of Final Research Achievements |
In this project, we intended to elucidate the function of secretory proteins localized to sporozoite rhoptries during the host invasion process. We identified that 8 secretory proteins are expressed in sporozoites and localized to rhoptries by immuno-electron microscopic analysis. Since most of rhoptry proteins have been believed to be essential for blood stage parasite proliferation, we generated “sporozoite stage-specific gene silencing parasites” by promoter swapping technique in order to analyze their functions during sporozoite development and/ or invasion of the mosquito salivary glands. As a result, we demonstrated that 5 rhoptry proteins play an important role in the mosquito salivary gland invasion by sporozoites.
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