| Co-Investigator(Kenkyū-buntansha) |
清水 健 千葉大学, 医学(系)研究科(研究院), 准教授 (70312840)
八尋 錦之助 千葉大学, 医学(系)研究科(研究院), 准教授 (80345024)
津々木 博康 大阪府立大学, 理学(系)研究科(研究院), 助教 (40586608)
小倉 康平 千葉大学, 大学院・医学研究院, 特任研究院 (00586612)
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| Budget Amount *help |
¥18,720,000 (Direct Cost: ¥14,400,000、Indirect Cost: ¥4,320,000)
Fiscal Year 2015: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2014: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2013: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2012: ¥7,020,000 (Direct Cost: ¥5,400,000、Indirect Cost: ¥1,620,000)
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| Outline of Final Research Achievements |
Subtilase cytotoxin (SubAB) is mainly produced by locus of enterocyte effacement (LEE) -negative strains of Shiga-toxigenic Escherichia coli (STEC). SubAB cleaves an endoplasmic reticulum (ER) chaperone, BiP/Grp78, leading to induction of ER stress. This stress causes activation of ER stress sensor proteins and induction of apoptosis. Here we showed that 1) SubAB enters the cells via lipid rafts- and an actin-dependent pathways 2)in mouse macrophages, that SubAB inhibited LPS-stimulated NO production through inhibition of NF-kB nuclear translocation and iNOS expression 3)SubAB-induced SG formation is regulated by the PERK/DAP1 signaling pathway, which may be modulated by PKCdelta/PKD1.
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