| Budget Amount *help |
¥17,940,000 (Direct Cost: ¥13,800,000、Indirect Cost: ¥4,140,000)
Fiscal Year 2014: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Fiscal Year 2013: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000)
Fiscal Year 2012: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
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| Outline of Final Research Achievements |
Type III secretion systems (T3SSs) are highly conserved in Bordetella pertussis and its related strain, B. bronchiseptica. This machinery delivers effectors into host cells leading to the long term colonization on trachea. Using a CyaA-mediated translocation assay, we demonstrated that BopN has an ability to translocate into the host cells. Furthermore, a pull-down assay revealed that BopN can associate with BopC. Interestingly, BopC-induced host-cell death is facilitated by the BopN localization into host cells.
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