Analysis of propagation mechanisms of hepatitis C virus and establishment of novel permissive cell lines
| Project/Area Number |
24390113
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Virology
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| Research Institution | Osaka University |
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Principal Investigator |
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥18,720,000 (Direct Cost: ¥14,400,000、Indirect Cost: ¥4,320,000)
Fiscal Year 2014: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
Fiscal Year 2013: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000)
Fiscal Year 2012: ¥6,890,000 (Direct Cost: ¥5,300,000、Indirect Cost: ¥1,590,000)
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| Keywords | HCV / replication / permissive cell line / αヘリックス / 宿主因子 / 複製 / 粒子産生 / 宿主側因子 |
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| Outline of Final Research Achievements |
Previous studies have shown that liver-specific host factors are required for efficient replication of HCV RNA and formation of infectious particles. In this study, we screened human cancer cell lines for expression of the liver-specific α-fetoprotein by using a cDNA array database, and identified novel permissive cell lines for a complete propagation of HCVcc without any artificial manipulation. In particular, gastric cancer-derived FU97 cells exhibited a much higher susceptibility to HCVcc/JFH-2 infection than was observed in Huh7 cells, suggesting that FU97 cells would be useful for further investigation of the HCV lifecycle, as well as the development of therapeutic agents for chronic hepatitis C.
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Report
(4 results)
Research Products
(39 results)
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[Journal Article] Amphipathic α-Helices in Apolipoproteins Are Crucial to the Formation of Infectious Hepatitis C Virus Particles2014
Author(s)
Fukuhara T, Wada M, Nakamura S, Ono C, Shiokawa M, Yamamoto S, Motomura T, Okamoto T, Okuzaki D, Yamamoto M, Saito I, Wakita T, Koike K, Matsuura Y
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Journal Title
PLoS Pathog
Volume: 10(12)
Issue: 12
Pages: e1004534-e1004534
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Innate immune response induced by baculovirus attenuates transgene expression in mammalian cells.2014
Author(s)
Ono C., Ninomiya A., Yamamoto S., Abe T., Wen X., Fukuhara T., Sasai M., Yamamoto M., Saitoh T., Satoh T., Kawai T., Ishii J. K., Akira S., Okamoto T., and Matsuura Y.
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Journal Title
Journal of Virology
Volume: 88
Issue: 4
Pages: 2157-2167
DOI
Related Report
Peer Reviewed
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