Tissue macrophages are responsible for inflammatory liver disease in the hepatitis C virus transgenic mice

• Project/Area Number: 24390117
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Virology
• Research Institution: Tokyo Metropolitan Institute of Medical Science
• Principal Investigator: KOHARA Michinori 公益財団法人東京都医学総合研究所, ゲノム医科学研究分野, プロジェクトリーダー (10250218)
• Co-Investigator(Kenkyū-buntansha): HIRATA Yuichi 公益財団財団法人東京都医学総合研究所, ゲノム医科学研究分野, 研究員 (50439452) ; YASUI Fumihiko 公益財団財団法人東京都医学総合研究所, ゲノム医科学研究分野, 主席研究員 (40399473) ; MUNAKATA Tsubasa 公益財団財団法人東京都医学総合研究所, ゲノム医科学研究分野, 主席研究員 (50420237)
• Project Period (FY): 2012-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥18,720,000 (Direct Cost: ¥14,400,000、Indirect Cost: ¥4,320,000); Fiscal Year 2014: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000); Fiscal Year 2013: ¥6,240,000 (Direct Cost: ¥4,800,000、Indirect Cost: ¥1,440,000); Fiscal Year 2012: ¥7,020,000 (Direct Cost: ¥5,400,000、Indirect Cost: ¥1,620,000)
• Keywords: C型肝炎ウイルス / 持続感染化 / 宿主免疫応答 / ウイルス排除 / 免疫学 / Ｃ型肝炎ウイルス / 脂肪変性

Outline of Final Research Achievements

We have reported that hepatitis C virus transgenic mice (HCV Tg) caused continuous liver injury and developed hepatocellular carcinoma through the Cre/loxP switching system. In addition, we showed recombinant vaccinia viruses expressing HCV nonstructural protein (rVV-N25) could protect against the progression of chronic hepatitis by way of suppression of macrophages activation. We focused on the role of tissue macrophages for liver disease of the HCV Tg mice and examined characteristic features of macrophages following rVV-N25 treatment. rVV-N25 treatment suppressed cell number and cytokine production on macrophages in the liver. M2 macrophages contribute to the induction of chronic liver inflammation in HCV mouse models. In addition, rVV-N25 induced therapeutic effect on liver tissue due to suppressed macrophage recruitment and activation.

Research Products

• [Journal Article] Property of hepatitis B virus replication in Tupaia belangeri hepatocytes (2016)
  • Author(s): Takahiro Sanada, Kyoko Tsukiyama-Kohara, Naoki Yamamoto, Sayeh Ezzikouri, Soumaya Benjelloun, Shuko Murakami, Yasuhito Tanaka, Chise Tateno, and Michinori Kohara
  • Journal Title: Biochem Biophys Res Commun Volume: 469 Issue: 2 Pages: 229-235
  • DOI: 10.1016/j.bbrc.2015.11.121
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] 3beta-hydroxysterol delta24-reductase on the surface of hepatitis C virus-related hepatocellular carcinoma cells can be a target for molecular targeting therapy (2015)
  • Author(s): M. Saito, T. Takano, T. Nishimura, M. Kohara, K. Tsukiyama-Kohara
  • Journal Title: PLoS ONE Volume: 10 Issue: 4 Pages: e0124197-e0124197
  • DOI: 10.1371/journal.pone.0124197
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Serum DHCR24 Auto-antibody as a new Biomarker for Progression of Hepatitis C. (2015)
  • Author(s): Ezzikouri S, Kimura K, Sunagozaka H, Kaneko S, Inoue K, Nishimura T, Hishima T, Kohara M, Tsukiyama-Kohara K.
  • Journal Title: EBioMedicine Volume: Apr 13;2(6) Issue: 6 Pages: 604-12
  • DOI: 10.1016/j.ebiom.2015.04.007
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] M2 macrophages play critical roles in progression of inflammatory liver disease in hepatitis C virus transgenic mice. (2015)
  • Author(s): 1.Ohtsuki T, Kimura K, Tokunaga Y, Tsukiyama-Kohara K, Tateno C, Hayashi Y, Hishima T, Kohara M
  • Journal Title: J Virol Volume: Oct 14;90(1) Issue: 1 Pages: 300-7
  • DOI: 10.1128/jvi.02293-15
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Inhibitory Effects of Pycnogenol on Hepatitis C Virus Replication. (2015)
  • Author(s): Ezzikouri S, Nishimura T, Kohara M, Benjelloun S, Kino Y, Inoue K, Matsumori A, Tsukiyama-Kohara K
  • Journal Title: Antiviral Research Volume: 113C Pages: 93-102
  • DOI: 10.1016/j.antiviral.2014.10.017
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] The RNA Sensor RIG-I Dually Functions as an Innate Sensor and Direct Antiviral Factor for Hepatitis B Virus (2015)
  • Author(s): Sato S, Li K, Kameyama T, Hayashi T, Ishida Y, Murakami S, Watanabe T, Iijima S, Sakurai Y, Watashi K, Tsutsumi S, Sato Y, Akita H, Wakita T, Rice CM, Harashima H, Kohara M, Tanaka Y, Takaoka A
  • Journal Title: Immunity Volume: 42 Issue: 1 Pages: 123-132
  • DOI: 10.1016/j.immuni.2014.12.016
  • Peer Reviewed / Open Access
• [Journal Article] Recent insights into hepatitis B virus-host interactions. (2014)
  • Author(s): Ezzikouri S, Ozawa M, Kohara M, Elmdaghri N, Benjelloun S, Tsukivama-Kohara K.
  • Journal Title: Journal of Medical Virology Volume: 86 Issue: 6 Pages: 925-32
  • DOI: 10.1002/jmv.23916
  • Peer Reviewed / Open Access
• [Journal Article] B-cell-intrinsic hepatitis C virus expression leads to B-cell-lymphomagenesis and induction of NF-κB signalling (2014)
  • Author(s): Kasama Y, Mizukami T, Kusunoki H, Peveling-Oberhag J, Nishito Y, Ozawa M, Kohara M, Mizuochi T, Tsukiyama-Kohara K
  • Journal Title: PLoS One Volume: 9(3) Issue: 3 Pages: 91373-91373
  • DOI: 10.1371/journal.pone.0091373
  • Peer Reviewed / Open Access
• [Journal Article] In vivo therapeutic potential of Dicer-hunting siRNAs targeting infectious hepatitis C virus (2014)
  • Author(s): Watanabe T, Hatakeyama H, Matsuda-Yasui C, Sato Y, Sudoh M, Takagi A, Hirata Y, Ohtsuki T, Arai M, Inoue K, Harashima H, Kohara M
  • Journal Title: Scientific Reports Volume: in press Issue: 1 Pages: 4750-4750
  • DOI: 10.1038/srep04750
  • Peer Reviewed / Open Access
• [Journal Article] Resistance to cyclosporin A derives from mutations in hepatitis C virus nonstructural proteins. (2014)
  • Author(s): Arai M, Tsukiyama-Kohara K, Takagi A, Tobita Y, Inoue K, Kohara M
  • Journal Title: Biochem Biophys Res Commun. Volume: 23 Issue: 1 Pages: 56-62
  • DOI: 10.1016/j.bbrc.2014.04.053
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Suppression of hepatitis C virus replication by cyclin-dependent kinase inhibitors (2014)
  • Author(s): Tsubasa Munakata, Makoto Inada, Yuko Tokunaga, Takaji Wakita, Michinori Kohara, Akio Nomoto
  • Journal Title: Antiviral Research Volume: 108 Pages: 79-87
  • DOI: 10.1016/j.antiviral.2014.05.011
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Nonstructural Protein 5A Is Incorporated into Hepatitis C Virus Low-Density Particle through Interaction with Core Protein and Microtubules during Intracellular Transport (2014)
  • Author(s): Chao-Kuen Lai, Vikas Saxena, Chung-Hsin Tseng, King-Song Jeng, Michinori Kohara, Michael M. C. Lai
  • Journal Title: PLoS ONE Volume: 9 Issue: 6 Pages: e99022-e99022
  • DOI: 10.1371/journal.pone.0099022
  • Peer Reviewed / Open Access
• [Journal Article] Tupaia belangeri as an experimental animal model for viral infection (2014)
  • Author(s): Kyoko Tsukiyama-Kohara, Michinori Kohara
  • Journal Title: Experimental Animals Volume: 63 Pages: 367-374
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Targeted induction of interferon-λ in humanized chimeric mouse liver abrogates hepatotropic virus infection (2013)
  • Author(s): Nakagawa S, Hirata Y, Kameyama T, Tokunaga Y, Nishito Y, Hirabayashi K, Yano J, Ochiya T, Tateno C, Tanaka Y, Mizokami M, Tsukiyama-Kohara K, Inoue K, Yoshiba M, Takaoka A, Kohara M
  • Journal Title: PLoS One Volume: 8 Issue: 3 Pages: e59611-e59611
  • DOI: 10.1371/journal.pone.0059611
  • Peer Reviewed
• [Journal Article] A serine palmitoyltransferase inhibitor blocks hepatitis C virus replication in human hepatocytes. (2013)
  • Author(s): Katsume A, Tokunaga Y, Hirata Y, Munakata T, Saito M, Hayashi H, Okamoto K, Ohmori Y, Kusanagi I, Fujiwara S, Tsukuda T, Aoki Y, Klumpp K, Tsukivama-Kohara K, El-Gohary A, Sudoh M, Kohara M.
  • Journal Title: Gastroenterology Volume: 145(4) Issue: 4 Pages: 865-79
  • DOI: 10.1053/j.gastro.2013.06.012
  • Peer Reviewed
• [Journal Article] DNA vaccine expressing the non-structural proteins of hepatitis C virus diminishes the expression of HCV proteins in a mouse model (2013)
  • Author(s): Takeshi Wada, Michinori Kohara and Yasuhiro Yasutomi
  • Journal Title: Vaccine Volume: 31 Issue: 50 Pages: 5968-5974
  • DOI: 10.1016/j.vaccine.2013.10.037
  • Peer Reviewed
• [Journal Article] Isolation and characterization of highly replicable hepatitis C virus genotype 1a strain HCV-RMT (2013)
  • Author(s): Masaaki Arai, Yuko Tokunaga, Asako Takagia, Yoshimi Tobita, Yuichi Hirata, Yuji Ishida, Chise Tateno, Michinori Kohara
  • Journal Title: PLoSONE Volume: 8 Issue: 12 Pages: e82527-e82527
  • DOI: 10.1371/journal.pone.0082527
  • Peer Reviewed
• [Journal Article] Synthetic lipophilic antioxidant BO-653 suppresses HCV replication. (2012)
  • Author(s): Yasui F.
  • Journal Title: J. Medical Virology Volume: 85 Issue: 2 Pages: 241-249
  • DOI: 10.1002/jmv.23466
  • Peer Reviewed
• [Journal Article] Kinetics of peripheral hepatitis B virus-specific CD8(+) T cells in patients with onset of viral reactivation. (2012)
  • Author(s): Aoki J, Kowazaki Y, Ohtsuki T, Okamoto R, Ohashi K, Hayashi S, Sakamaki H, Kohara M, Kimura K
  • Journal Title: J Gastroenterol Volume: 47 Issue: 6 Pages: 22-22
  • DOI: 10.1007/s00535-012-0676-y
  • NAID: 10031184063
  • Peer Reviewed
• [Journal Article] Hepatitis C virus kinetics by administration of pegylated interferon-lin human and chimeric mice carrying human hepatocytes with variants of the IL28B gene (2012)
  • Author(s): Watanabe T, Sugauchi F, Tanaka Y, Matsuura K, Yatsuhashi H, Murakami S, Iijima S, Iio E, Sugiyama M, Shimada T, Kakuni M, Kohara M, Mizokami M
  • Journal Title: Gut Volume: Nov 7 Issue: 9 Pages: 1340-6
  • DOI: 10.1136/gutjnl-2012-302553
  • Peer Reviewed
• [Journal Article] An orally available, small-molecule interferon inhibits viral replication (2012)
  • Author(s): Konishi H, et.al.
  • Journal Title: Scientific Reports Volume: 2 Issue: 1 Pages: 259-259
  • DOI: 10.1038/srep00259
  • Peer Reviewed
• [Journal Article] Hepatitis C virus promotes expression of the 3β-hydroxysterol δ24-reductase through Sp1. (2012)
  • Author(s): Saito M, Kohara, M, Tsukiyama-Kohara K.
  • Journal Title: J Med Virol Volume: 84 Issue: 5 Pages: 733-746
  • DOI: 10.1002/jmv.23250
  • Peer Reviewed
• [Journal Article] HSC90 is required for nascent hepatitis C virus core protein stability in yeast cells. (2012)
  • Author(s): Kubota N, Inayoshi Y, Satoh N. Fukuda T, Iwai K, Tomoda H, Kohara M, Kataoka K, Shimamoto A, Furuichi Y, Nomoto A, Naganuma A, Kuge S
  • Journal Title: FEBS Letters Volume: (in press) Issue: 16 Pages: 2318-2325
  • DOI: 10.1016/j.febslet.2012.05.023
  • Peer Reviewed
• [Journal Article] PA from an H5N1 highly pathogenic avian influenza virus activates viral transcription and replication, and induces apoptosis and interferon expression. (2012)
  • Author(s): Wang Q.
  • Journal Title: Virology Journal Volume: 9 Issue: 1 Pages: 106-118
  • DOI: 10.1186/1743-422x-9-106
  • Peer Reviewed
• [Journal Article] Self-enhancement of hepatitis C virus replication by promotion of specific sphingolipid biosynthesis (2012)
  • Author(s): Hirata Y, Ikeda K, shimotohno K, et al.,
  • Journal Title: PLoS Pathog Volume: PLoS Pathog Issue: 8 Pages: e1002860-e1002860
  • DOI: 10.1371/journal.ppat.1002860
  • Peer Reviewed
• [Journal Article] Impairment of interferon regulatoroy factor-3 activation by hepatitis C virus core protein basic amino acid region 1. (2012)
  • Author(s): Inoue K, Tsukiyama-Kohara K, Matsuda C, Yoneyama M, Fujita T, Kuge S, Yoshida M, Kohara M
  • Journal Title: Bichem Biophys Res Commun Volume: 428 Issue: 4 Pages: 494-499
  • DOI: 10.1016/j.bbrc.2012.10.079
  • Peer Reviewed
• [Journal Article] Immunization with a recombinant vaccinia virus that encodes nonstructural proteins of the hepatitis C virus suppresses viral protein levels in mouse liver. (2012)
  • Author(s): Sekiguchi, S, Kimura K, Chiyo T, Ohtsuki T, Tobita Y, Tokunaga Y, Yasui F, Tsukiyama-Kohara K, Wakita T, Tanaka T, Miyasaka M, Mizuno K, Hayashi Y, Hishima T, Matsushima K and Kohara M.
  • Journal Title: PLoS One Volume: 7 Issue: 12 Pages: e51656-e51656
  • DOI: 10.1371/journal.pone.0051656
  • Peer Reviewed
• [Journal Article] Hepatitis C virus kinetics by administration of pegylated interferon-α in human and chimeric mice carrying human hepatocytes with variants of the IL28B gene (2012)
  • Author(s): Watanabe T.
  • Journal Title: Gut Volume: (In press)
  • Peer Reviewed
• [Journal Article] Different mechanisms of hepatitis C virus RNA polymerase activation by cyclophilin A and B in vitro (2011)
  • Author(s): Weng L, Tian X, shimotohno K, et al.
  • Journal Title: Biochim Biophys Acta Volume: 1820 Issue: 12 Pages: 1886-1892
  • DOI: 10.1016/j.bbagen.2012.08.017
  • Peer Reviewed
• [Presentation] M2 macrophages play critical roles in progression of inflammatory liver disease in hepatitis C virus (2015)
  • Author(s): Takahiro Ohtsuki, Kiminori Kimura, Yuko Tokunaga, Kyoko Tsukiyama-Kohara, Michinori Kohara
  • Organizer: 22nd International Symposium on Hepatitis C virus and Related viruses
  • Place of Presentation: Strasbourg (France)
  • Year and Date: 2015-10-09
  • Int'l Joint Research
• [Presentation] DHCR24 on the surface of HCV-related hepatocellular carcinoma cells can be a target for molecular targeting therapy (2015)
  • Author(s): Makoto Saito, Takashi Takano, Tomohiro Nishimura, Michinori Kohara, Kyoko Tsukiyama-Kohara
  • Organizer: 第74回日本癌学会学術総会
  • Place of Presentation: 名古屋国際会議場（愛知県・名古屋市）
  • Year and Date: 2015-10-08
• [Presentation] Exosomes contain HBV DNA and HBs Ag (2015)
  • Author(s): Takahiro Sanada, Yuichi Hirata, Yutaka Naito, Naoki Yamamoto, Yuji Ishida, Chihiro Yamasaki, Chise Tateno, Takahiro Ochiya, michinori Kohara
  • Organizer: 2015International Meeting on Molecular Biology of Hepatitis B Viruses
  • Place of Presentation: Dolce Bad Nauheim (Germany)
  • Year and Date: 2015-10-04
  • Int'l Joint Research
• [Presentation] Selective inhibitor of Wnt/β-catenin/CBP signaling ameliotates hepatitis C virus-induced liver fibrosis in mice (2015)
  • Author(s): Kiminori Kimura, Yuko Tokunaga, Takahiro Ohtuki, Yukiko Hayashi, Keisuke Munekata, Tsunekazu Hishima, Michinori Kohara
  • Organizer: 50th The International Liver Congress (EASL)
  • Place of Presentation: Vienna (Austria)
  • Year and Date: 2015-04-22
  • Int'l Joint Research
• [Presentation] Immunization with a recombinant vaccinia virus encoding a nonstructural protein of the hepatitis C virus suppresses viral protein level in mouse liver (2015)
  • Author(s): Kiminori Kimura, Takahiro Otsuki, Yuko Tokunaga, Kyoko Tsukiyama-Kohara, Michinori Kohara
  • Organizer: 第5回発がんスパイラル国際シンポジウム
  • Place of Presentation: 神戸ポートピアホテル〔兵庫）
  • Year and Date: 2015-02-26 – 2015-02-27
• [Presentation] A Serine palmitoyltransferase inhibitor inhibits hepatitis C virus replication in human hepatocytes (2014)
  • Author(s): Michinori Kohara, Yuko Tokunaga, Tsubasa Munakata, Kyoko Tsukiyama-Kohara, Masayuki Sudoh
  • Organizer: 第37回日本分子生物学会
  • Place of Presentation: パシフィコ横浜（神奈川）
  • Year and Date: 2014-11-25 – 2014-11-27
• [Presentation] C型肝炎ウイルス感染時にTLR3はIFN非依存的に発現して抗ウイルス作用を示す (2014)
  • Author(s): 棟方 翼、徳永優子、真田崇弘、脇田隆字、野本明男、小原道法
  • Organizer: 第62回日本ウイルス学会学術集会
  • Place of Presentation: パシフィコ横浜〔神奈川）
  • Year and Date: 2014-11-10 – 2014-11-12
• [Presentation] Inhibitory effects of pycnogenol on Hepatitis C Virus replication (2014)
  • Author(s): Sayeh Ezzikouri, Michinori Kohara, Kyoko Tsukiyama-Kohara
  • Organizer: 第73回日本癌学会学術総会
  • Place of Presentation: パシフィコ横浜 (神奈川）
  • Year and Date: 2014-09-25 – 2014-09-27
• [Presentation] Resistance to cyclosporine S derives from mutations in Hepatitis C virus nonstructural proteins (2014)
  • Author(s): Masaaki Arai, Kyoko Tsukiyama-Kohara, Asako Takagi, Yoshimi Tobita, Kazuaki Inoue, Michinori Kohara
  • Organizer: 21th International Symposium on Hepatitis C and Related Virus
  • Place of Presentation: Banff（Canada）
  • Year and Date: 2014-09-07 – 2014-09-11
• [Presentation] Selective inhibitor of Wnt/β-catenin/CBP signaling ameliorates hepatitis C virus-induced liver fibrosis (2014)
  • Author(s): Yuko Tokunaga, Kiminori Kimura, Takahiro Ohtsuki, Yukiko Hayashi, Mitsuko Hara, Keisuke Munekata, Tsunekazu Hishima, Soichi Kojima, Michinori Kohara
  • Organizer: 21th International Symposium on Hepatitis C and Related Virus
  • Place of Presentation: Banff（Canada）
  • Year and Date: 2014-09-07 – 2014-09-11
• [Presentation] Suppression of hepatitis C virus replication by cyclin-dependent kinase inhibitors (2014)
  • Author(s): Tubasa Munakata, Makoto Inada, Yuko Tokunaga, Takaji Wakita, Michinori Kohara, Akio Nomoto
  • Organizer: 21th International Symposium on Hepatitis C and Related Virus
  • Place of Presentation: Banff（Canada）
  • Year and Date: 2014-09-07 – 2014-09-11
• [Presentation] HBV Pathogenesis and host response in Tupaia belangeri (2014)
  • Author(s): Takahiro Sanada, Kyoko Tsukiyama-Kohara, S Ezzikouri, Naoki Yamamaoto, Michinori Kohara
  • Organizer: 2014 Internationl Meeting on Molecular Biology of Hepatitis B Viruses
  • Place of Presentation: Los Angeles (USA)
  • Year and Date: 2014-09-03 – 2014-09-06
• [Presentation] Development of Tupaia Belangeri for small animal infection model of hepatitis B virus, according to the genomic research (2014)
  • Author(s): Takahiro Sanada, Kyoko Tsukiyama-Kohara, S Ezzikouri, Naoki Yamamaoto, Michinori Kohara
  • Organizer: XVIth International Congress of Virology
  • Place of Presentation: Montreal (Canada)
  • Year and Date: 2014-07-27 – 2014-08-01
• [Presentation] Comprehensive analysis of B-lymphoma cells spontaneously developed in transgenic mice that express the full hepatitis C virus genome in B cells (2014)
  • Author(s): Kyoko Tsukiyama-Kohara, Michinori Kohara, Yuri Kasama
  • Organizer: XVIth International Congress of Virology
  • Place of Presentation: Montreal (Canada)
  • Year and Date: 2014-07-27 – 2014-08-01
• [Presentation] 免疫の賦活化によるウイルス性肝炎治療及び肝線維症治療に向けて (2014)
  • Author(s): 小原道法
  • Organizer: 第9回高輪ウイルス肝炎フォーラム
  • Place of Presentation: JCHO東京高輪病院 (東京)
  • Year and Date: 2014-06-14
  • Invited
• [Presentation] Non-DAAセリンパルミトイル基転移酵素阻害剤とDAA併用による薬剤耐性株の出現を押さえた強力なHCV複製阻害 (2014)
  • Author(s): 小原道法、徳永優子、須藤正幸
  • Organizer: 第50回日本肝臓学会総会
  • Place of Presentation: ホテルニューオータニ（東京）
  • Year and Date: 2014-05-29
• [Presentation] C型肝炎・肝硬変発症機序及びその制御を目指して (2014)
  • Author(s): 小原 道法
  • Organizer: 第22回桜山感染症研究会
  • Place of Presentation: 名古屋
  • Invited
• [Presentation] ワクシニアLC16m8株（HCV,SARS,Influenza）ベクター (2013)
  • Author(s): 小原 道法
  • Organizer: 第17回日本ワクチン学会学集会
  • Place of Presentation: 三重
  • Invited
• [Presentation] Immunization with a recombinant vaccinia virus encoding a nonstructural protein of the hepatitis C virus ameliorates chronic hepatitis in the liver of transgenic mice (2012)
  • Author(s): Kimura K.
  • Organizer: Keystone Symposia on Molecular and Cellular Biology
  • Place of Presentation: Ottawa(Canada)
  • Year and Date: 2012-12-15
• [Presentation] Self-enhancement of hepatitis C Virus replication by promotion of specific sphingolipid biosynthesis (2012)
  • Author(s): Hirata Y.
  • Organizer: 第35回日本分子生物学会年会
  • Place of Presentation: 福岡国際会議場
  • Year and Date: 2012-12-12
• [Presentation] Single immunization with H5N1 influenza vaccine based on recombinant vaccinia virus protects mice and macaques from challenge with H5N1 in fluenza virus (2012)
  • Author(s): Yasui F.
  • Organizer: 第41回日本免疫学会学術集会
  • Place of Presentation: 福岡国際会議場
  • Year and Date: 2012-12-07
• [Presentation] C型肝炎モデルマウスを用いた治療用DNAワクチンの検討 (2012)
  • Author(s): 和田 剛
  • Organizer: 第16回日本ワクチン学会学術集会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2012-11-18
• [Presentation] HCV感染による慢性肝炎の病態形成と治療ワクチン (2012)
  • Author(s): 小原 道法
  • Organizer: 第16回日本ワクチン学会学術集会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2012-11-18
• [Presentation] カニクイザルモデルでのH5N1高病原性トリインフルエンザ組換えワクシニアワクチン単回接種による発症防御効果の検討 (2012)
  • Author(s): 安井 文彦
  • Organizer: 第16回日本ワクチン学会学術集会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2012-11-17
• [Presentation] C型肝炎ウイルス感染時にTool-Like Receptor 3 の発現はRb/E2F経路で制御される (2012)
  • Author(s): 棟方 翼
  • Organizer: 第60回日本ウイルス学会学術集会
  • Place of Presentation: グランキューブ大阪
  • Year and Date: 2012-11-15
• [Presentation] H5N1高病原性トリインフルエンザ組換えワクシニアワクチン単回接種による発症防御効果の検討 (2012)
  • Author(s): 宗片 圭祐
  • Organizer: 第60回日本ウイルス学会学術集会
  • Place of Presentation: グランキューブ大阪
  • Year and Date: 2012-11-13
• [Presentation] C型肝炎ウイルスによるスフィンゴ脂質の合成促進とウイルス複製環境の構築 (2012)
  • Author(s): 平田 雄一
  • Organizer: 第60回日本ウイルス学会学術集会
  • Place of Presentation: グランキューブ大阪
  • Year and Date: 2012-11-13
• [Presentation] 新世代抗体医薬としての特殊環状ペプチドによるインフルエンザウイルス増殖阻害効果 (2012)
  • Author(s): 斉藤 誠
  • Organizer: 第60回日本ウイルス学会学術集会
  • Place of Presentation: グランキューブ大阪
  • Year and Date: 2012-11-13
• [Presentation] Development of recombinant H5N1 influenza vaccine based on vaccinia virus vector (2012)
  • Author(s): Yasui F.
  • Organizer: 6^<th> Vaccine & ISV Congress
  • Place of Presentation: Shanghai(China)
  • Year and Date: 2012-10-15
• [Presentation] Self-enhancement of Hepatitis C Virus replication by promotion of specific sphingolipid biosynthesis (2012)
  • Author(s): Hirata Y.
  • Organizer: 19^<th> International Symposium on Hepatitis C Virus and Related Viruses
  • Place of Presentation: Venice(Italy)
  • Year and Date: 2012-10-08
• [Presentation] Hepatic toll-like receptor 3 induced by E2F1 controls hepatitisC viral replication (2012)
  • Author(s): Munakata T.
  • Organizer: 19^<th> International Symposium on Hepatitis C Virus and Related Viruses
  • Place of Presentation: Venice(Italy)
  • Year and Date: 2012-10-08
• [Presentation] Antibody against 3 β-Hydroxysterol-δ 24-reductase suppresses Hepatitis C virus infection through Betaine/GABA transporter-1 (2012)
  • Author(s): Satoh M.
  • Organizer: 19^<th> International Symposium on Hepatitis C Virus and Related Viruses 2012.10.4-11
  • Place of Presentation: Venice(Italy)
  • Year and Date: 2012-10-08
• [Presentation] Immunization with a recombinant vaccinia virus encoding a nonstructural protein of the Hepatitis C Virus suppresses viral protein level in mouse liver (2012)
  • Author(s): Kimura K.
  • Organizer: 19^<th> International Symposium on Hepatitis C Virus and Related Viruses 2012.10.4-11
  • Place of Presentation: Venice(Italy)
  • Year and Date: 2012-10-07
• [Presentation] C型肝炎ウイルスの複製に関与する新規宿主因子BGT-1 (2012)
  • Author(s): 小原 恭子
  • Organizer: 第71回日本癌学会学術総会
  • Place of Presentation: ロイトン札幌
  • Year and Date: 2012-09-19
• [Presentation] Persistent expression of the full genome of hepatitis C virus in C cells induces spontaneous development of B-cell lymphomas in vivo (2012)
  • Author(s): Tsukiyama-Kohara K.
  • Organizer: 第11回あわじしま感染症・免疫フォーラム
  • Place of Presentation: 淡路夢舞台国際会議場(兵庫)
  • Year and Date: 2012-09-12
• [Presentation] Development of Pandemic Influenza Vaccines based on Recombinant Vaccinia Virus (2012)
  • Author(s): Yasui F.
  • Organizer: 2012 TEPIK International Influenza Symposium
  • Place of Presentation: Seoul(KOREA)(招待講演)
  • Year and Date: 2012-06-29
• [Presentation] cDNA-uPA/SCIDマウスをホストとした長期試験に適するヒト肝細胞キメラマウスの開発 (2012)
  • Author(s): 大下浩樹
  • Organizer: 第59回日本実験動物学会総会
  • Place of Presentation: 別府国際コンベンションセンター(大分)
  • Year and Date: 2012-05-24
• [Presentation] ウイルス性肝炎の免疫学的制御に向けて (2012)
  • Author(s): 小原 道法
  • Organizer: 第5回高輪ウイルス肝炎フォーラム
  • Place of Presentation: せんぽ東京高輪病院(招待講演)
  • Year and Date: 2012-04-28
• [Presentation] Augmentation of DHCR24 expression by hepatitis C virus infection facilitates viral replication in hepatocytes (2012)
  • Author(s): Takano T.
  • Organizer: The International Liver Congress
  • Place of Presentation: Barcelona(SPAIN)
  • Year and Date: 2012-04-20
• [Book] ヒト肝細胞における強力なIFN-λ誘導による抗ウイルス効果 (2012)
  • Author(s): 平田 雄一
  • Publisher: 消化器内科
• [Book] C型肝炎ウイルス感染モデル (2012)
  • Author(s): 小原 道法
  • Publisher: 感染症
• [Book] HCV治療ワクチンの開発について (2012)
  • Author(s): 木村 公則
  • Publisher: 癌と科学療法社
• [Remarks] 公益財団法人東京都医学総合研究所
  • URL: http://www.igakuken.or.jp
• [Remarks] 公益財団法人東京都医学総合研究所プロジェクト研究
  • URL: http://www.igakuken.or.jp/project/detail/infectious.html
• [Remarks] 公益財団法人東京都医学総合研究所
  • URL: http://www.igakuken.or.jp/
• [Remarks]
  • URL: http://www.igakuken.or.jp/