Exploratory Research for Fetal Programming Effects by Using Omics Analysis including Epigenomics

• Project/Area Number: 24390144
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Laboratory medicine
• Research Institution: Hamamatsu University School of Medicine
• Principal Investigator: MAEKAWA Masato 浜松医科大学, 医学部, 教授 (20190291)
• Co-Investigator(Kenkyū-buntansha): WATANABE Hiroshi 浜松医科大学, 医学部, 教授 (50262803)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥18,590,000 (Direct Cost: ¥14,300,000、Indirect Cost: ¥4,290,000); Fiscal Year 2014: ¥4,030,000 (Direct Cost: ¥3,100,000、Indirect Cost: ¥930,000); Fiscal Year 2013: ¥6,630,000 (Direct Cost: ¥5,100,000、Indirect Cost: ¥1,530,000); Fiscal Year 2012: ¥7,930,000 (Direct Cost: ¥6,100,000、Indirect Cost: ¥1,830,000)
• Keywords: グライコミクス / グリコブロッティング / 胎児プログラミング / 低出生体重児 / 不当軽量児 / N-グリカン / 予知医学 / エピゲノミクス / 予防医学 / 極低出生体重児 / グライコブロッティング法 / オミックス

Outline of Final Research Achievements

To investigate which cord blood N-glycans are associated with poor fetal environment indicators including LBW and SGA, or rapid weight gain in early infancy. From a regional prospective birth cohort, 36 LBW neonates and 120 normal-birth-weight neonates were recruited. A comprehensive glycoblotting using MALDI-TOF-MS-based N-glycan analysis in cord blood from these neonates were conducted. A total of 35 N-glycans were detected (m/z 1362.481-3865.407). Of these, G3414 were correlated with both LBW and SGA in logistic regression. G1915, G2744, G3049 and G3719 were associated with LBW. In the prospective follow-up analysis, these 5 N-glycans were all associated with 6 and 18-months rapid weight gain after birth. These identified candidate N-glycans are structurally categorized into two different categories. These N-glycans were suggestive of potential predictors of poor fetal environment or rapid weight gain after birth.

Research Products

• [Journal Article] Pustular psoriasis-like lesions associated with hereditary lactate dehydrogenase M subunit deficiency without interleukin-36 receptor antagonist mutation: long-term follow-up of two cases. (2015)
  • Author(s): Ito T , Aoshima M, Sugiura K, Fujiyama T, Ito N, Sakabe JI, Akiyama M, Maekawa M, Tokura Y
  • Journal Title: Br J Dermatol. Volume: Epub Issue: 6 Pages: 1674-6
  • DOI: 10.1111/bjd.13590
  • Peer Reviewed / Open Access
• [Journal Article] Recommended use of Cut-off Folate Concentration in Serum and Erythrocyte(Red Blood Cell) as Expressed by Folic Acid Equivalent for the Diagnosis of Deficiency in Deliberating the Creation on Dietary Reference Intakes. (2015)
  • Author(s): Ihara H, Hirota K, Watanabe T, Totani M, Hashizume N, Aoki Y, Nagashima Y, Kamioka K, Onda K, Sunahara S, Suzuki T, Itabashi M, Ishibashi M, Ito S, Ohashi K, Ohta Y, Nobori T, Fujishiro K, Maekawa M, Miura M, Miyano H, Ando T, Nishimura K
  • Journal Title: Austin J Nutri Food Sci Volume: 3(1) Pages: 1055-1056
  • Peer Reviewed / Open Access
• [Journal Article] HbA1c NGSP値のサーベイ (2015)
  • Author(s): 村上正巳、柏木厚典、武井 泉、雨宮 伸、石橋みどり、植木浩二郎、佐藤麻子、永峰康孝、前川真人、矢冨 裕
  • Journal Title: 糖尿病 Volume: 58(1) Pages: 45-50
  • NAID: 130004905472
  • Peer Reviewed / Open Access
• [Journal Article] Final height and cardiometabolic outcomes in young adults with very low birth weight (< 1500 g). (2014)
  • Author(s): Sato R, Maekawa M, Genma R, Shirai K, Ohki S, Morita H, Suda T, Watanabe H
  • Journal Title: PLOS ONE Volume: 9 (11) Issue: 11 Pages: 1-9
  • DOI: 10.1371/journal.pone.0112286
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] National Institute of Standards and Technorogy SRM 972 as a reference material for serum total 25-hydroxyvitamin D measurements. (2014)
  • Author(s): Ihara H, Hirota K, Miura M, Kitajima I, Yamashita M, Nomura F, Nishimura M, Totani M, Hashizume N,Aoki Y, Nagashima Y, Kamioka K, Onda K, Sunahara S, Suzuki T, Itabashi M, Ishibashi M, Ito S, Ohashi K, Ohta Y, Nobori T, Fujishiro K, Maekawa M, Miyano H, Ando T, Nishimura K, Tsugawa N, Okano T :
  • Journal Title: Int Anal Bio-Sci Volume: 2 (1) Pages: 1-6
  • Peer Reviewed / Open Access
• [Journal Article] Plasma connective tissue growth factor levels as potential biomarkers of airway obstruction in patients with asthma. Ann Allergy Asthma Immunol. (2014)
  • Author(s): Kato M, Fujisawa T, Hashimoto D, Kono M, Enomoto N, Nakamura Y, Inui N, Hamada E, Miyazaki O, Kurashita S, Maekawa M, Suda T
  • Journal Title: Ann Allergy Asthma Immunol. Volume: 113 (3) Issue: 3 Pages: 295-300
  • DOI: 10.1016/j.anai.2014.05.026
  • Peer Reviewed / Open Access
• [Journal Article] Usual interstitial pneumonia preceding collagen vascular disease: a retrospective case control study of patients initially diagnosed with idiopathic pulmonary fibrosis. (2014)
  • Author(s): Kono M, Nakamura Y, Enomoto N, Hashimoto D, Fujisawa T, Inui N, Maekawa M, Suda T, Colby TV, Chida K
  • Journal Title: PLOS ONE Volume: 9 Issue: 4 Pages: e94775-e94775
  • DOI: 10.1371/journal.pone.0094775
  • Peer Reviewed / Open Access
• [Journal Article] IgG4-related skin disease. (2014)
  • Author(s): Tokura Y, Yagi H, Yanaguchi H, Majima Y, Kasuya A, Ito T, Maekawa M, Hashizume H
  • Journal Title: Br J Dermatol. Volume: 171 Issue: 5 Pages: 959-967
  • DOI: 10.1111/bjd.13296
  • Peer Reviewed / Open Access
• [Journal Article] Cell line differences in replication timing of human glutamate receptor genes and other large genes associated with neuraldisease. (2014)
  • Author(s): Watanabe Y, Shibata K, Maekawa M
  • Journal Title: Epigenetics. Volume: 9 (10) Pages: 1350-1359
  • Peer Reviewed / Open Access
• [Journal Article] 臨床検査医学 アルブミン測定、BCP改良法とBCG法による測定値 (2014)
  • Author(s): 前川真人
  • Journal Title: 医学のあゆみ Volume: 251(2) Pages: 182-183
  • Open Access
• [Journal Article] 骨型ALP測定法 (2014)
  • Author(s): 下田 綾、前川真人
  • Journal Title: 検査と技術 Volume: 42(13) Pages: 1382-1388
  • Open Access
• [Journal Article] 血清酵素検査で見出された異常データの電気泳動解析 (2014)
  • Author(s): 前川真人
  • Journal Title: 臨床病理 Volume: 62(11) Pages: 1088-1093
  • Peer Reviewed / Open Access
• [Journal Article] TLR-Mediated Airway IL-17C Enhances Epithelial Host Defense in an Autocrine/Paracrine Manner. (2014)
  • Author(s): Kusagaya H, Fujisawa T*, Yamanaka K, Mori K, Hashimoto D, Enomoto N, Inui N, Nakamura Y, Wu R, Maekawa M, Suda T, Chida K.
  • Journal Title: Am J Respir Cell Mol Biol. Volume: 50 Issue: 1 Pages: 30-39
  • DOI: 10.1165/rcmb.2013-0130oc
  • Peer Reviewed
• [Journal Article] Evaluation of various storage conditions of laboratory testing samples. (2013)
  • Author(s): Furuta K, Hashiguchi T, Hidaka Y, Kang D, Ikeda K, Maekawa M, Matsumoto H, Matsushita K, Okubo S, Tsuchiya T
  • Journal Title: Cryobiology Volume: 67 Issue: 3 Pages: 398-442
  • DOI: 10.1016/j.cryobiol.2013.09.152
  • Peer Reviewed
• [Journal Article] A sudden onset and the spontaneous remission of severe hypo-high-density lipoprotein cholesterolemia without serious underlying disease: a case report. (2013)
  • Author(s): Ohba K, Oki Y, Fujita K, Kameko F, Moriyama T, Horiike Y, Morita H, Matsushita A, Iino K, Sasaki S, Nakamura H, Maekawa M.
  • Journal Title: Clin Chim Acta. Volume: 15 Pages: 91-94
  • DOI: 10.1016/j.cca.2013.09.003
  • Peer Reviewed
• [Journal Article] Plasma exposure of free linezolid and its ratio to minimum inhibitory concentration varies in critically ill patients. (2013)
  • Author(s): Yagi T, Naito T, Doi M, Nagura O, Yamada T, Maekawa M, Sato S, Kawakami J
  • Journal Title: Int J Antimicrob Agents Volume: 42(4) Issue: 4 Pages: 329-334
  • DOI: 10.1016/j.ijantimicag.2013.06.015
  • Peer Reviewed
• [Journal Article] Alveolar nitric oxide concentration reflects peripheral airway obstruction in stable asthma. (2013)
  • Author(s): Fujisawa T, Yasui H, Akamatsu T, Hashimoto D, Enomoto N, Inui N, Nakamura Y, Maekawa M, Suda T, Chida K.
  • Journal Title: Respirology Volume: 18 Issue: 3 Pages: 522-7
  • DOI: 10.1111/resp.12031
  • Peer Reviewed
• [Journal Article] Laminin, gamma 2 (LAMC2): a promising new putative pancreatic cancer biomarker identified by proteomic analysis of pancreatic adenocarcinoma tissues. (2013)
  • Author(s): Kosanam H, Prassas I, Chrystoja CC, Soleas I, Chan A, Dimitromanolakis A, Blasutig IM, Rückert F, Gruetzmann R, Pilarsky C, Maekawa M, Brand R, Diamandis EP
  • Journal Title: Mol Cell Proteomics Volume: 12(10) Issue: 10 Pages: 2820-2832
  • DOI: 10.1074/mcp.m112.023507
  • Peer Reviewed
• [Journal Article] Serum levels of soluble platelet endothelial cell adhesion molecule-1 and vascular cell adhesion molecule-1 are decreased in subjects with autism spectrum disorder. (2013)
  • Author(s): Kameno Y, Iwata K, Matsuzaki H, Miyachi T, Tsuchiya KJ, Matsumoto K, Iwata Y, Suzuki K, Nakamura K, Maekawa M, Tsujii M, Sugiyama T, Mori N.
  • Journal Title: Mol Autism Volume: 4 Issue: 1 Pages: 19-19
  • DOI: 10.1186/2040-2392-4-19
  • Peer Reviewed
• [Journal Article] 腫瘍マーカー検査の最新情報 (2013)
  • Author(s): 前川真人
  • Journal Title: 日本内科学会雑誌 Volume: 102(12) Pages: 3133-3139
  • Peer Reviewed
• [Journal Article] 臨床検査における電気泳動技術の意義 (2013)
  • Author(s): 前川真人
  • Journal Title: 医療と検査機器・試薬 Volume: 36(3) Pages: 259-264
  • Peer Reviewed
• [Journal Article] アルカリ性ホスファターゼの測定 (2013)
  • Author(s): 前川真人
  • Journal Title: 検査と技術 Volume: 41(7) Pages: 596-597
  • Peer Reviewed
• [Journal Article] 固形腫瘍関連検査 (2013)
  • Author(s): 前川真人
  • Journal Title: 臨床検査 Volume: 57(7) Pages: 720-725
  • Peer Reviewed
• [Journal Article] HPV DNAの検出による感染の診断 (2013)
  • Author(s): 前川真人
  • Journal Title: 産科と婦人科 Volume: 80(6) Pages: 721-726
  • Peer Reviewed
• [Journal Article] R/G-band boundaries: Genomic instability and human disease (2013)
  • Author(s): Watanabe Y, Maekawa M
  • Journal Title: Clinica Chimica Acta Volume: 419 Pages: 108-112
  • DOI: 10.1016/j.cca.2013.02.011
  • Peer Reviewed
• [Journal Article] Methods and Strategies to Determine Epigenetic Variati on in Human Disease (2012)
  • Author(s): Watanabe Y, Maekawa M
  • Journal Title: Epigenetics in Human Disease Volume: 2 Pages: 2-27
  • DOI: 10.1016/b978-0-12-388415-2.00002-0
  • ISBN: 9780123884152
  • Peer Reviewed
• [Journal Article] Investigation of unexpected serum CA19-9 elevation in Lewis-negative cancer patients. (2012)
  • Author(s): Hamada E, Taniguchi T, Baba S, Maekawa M
  • Journal Title: Annals of Clinical Biochemistry Volume: 49(3) Issue: 3 Pages: 266-272
  • DOI: 10.1258/acb.2011.011213
  • Peer Reviewed
• [Journal Article] Hepatocellular adenoma associated with familial adenomatous polyposis coli. (2012)
  • Author(s): Inaba K, Sakaguchi T, Kurachi K, Mori H, Tao, H, Nakamura T, Takehara Y, Baba S, Maekawa M, Sugimura H, Konno H
  • Journal Title: World Journal of Hepatology Volume: 4(11) Issue: 11 Pages: 322-326
  • DOI: 10.4254/wjh.v4.i11.322
  • Peer Reviewed
• [Journal Article] エピジェネテイクスの検査 (2012)
  • Author(s): 前川真人
  • Journal Title: Medical Technology Volume: 40(13) Pages: 1630-1631
  • Peer Reviewed
• [Presentation] Analytical evaluation of third-generation allergen-specific IgE assay “3gAllergyTM” Measurement by Automated Immunoassey System “IMMULITE 2000XPi”. (2014)
  • Author(s): Hamada E, Maekawa M
  • Organizer: AACC 2014 Annual Meeting& Clinical Lab Expo
  • Place of Presentation: McCormic Place Convention Center, Chicago USA
  • Year and Date: 2014-07-27 – 2014-07-31
• [Presentation] A case of prostate cancer producing granulocyte colony- stimulating factor. (2014)
  • Author(s): Hamada E, Takayama T, Ozono S, , Maekawa M
  • Organizer: IFCC Worldlab Istanbul 2014
  • Place of Presentation: Istanbul Congress Center, Istanbul Turkey
  • Year and Date: 2014-06-22 – 2014-06-26
• [Presentation] Standardization for Laboratory Medicine and National EQA Survey Program in Japan (2012)
  • Author(s): Maekawa M
  • Organizer: Korean Association of Quality Assurance for Clinical Laboratory's 2012 autumn symposium
  • Place of Presentation: (招待講演)
  • Year and Date: 2012-09-14
• [Presentation] 極低出生体重児(Very low birth weight infants ; VLBW<1500g)の若年成人期における耐糖能 (2012)
  • Author(s): 佐藤亮介, 渡邉裕司, 白井憲司, 大木茂, 源馬理恵子, 森田浩, 井上永介, 竹内正弘, 前川真人, 中村浩淑
  • Organizer: 日本糖尿病学会
  • Place of Presentation: 横浜
  • Year and Date: 2012-05-17
• [Presentation] Laboratory automation system in molecular diagnostic testing (2012)
  • Author(s): Maekawa M
  • Organizer: The 8th International Conference of Clinical Laboratory Automation
  • Place of Presentation: Seoul, Korea(招待講演)
  • Year and Date: 2012-04-12
• [Presentation] Significance of routine matrix metalloproteinase-3 (MMP-3) measurement for follow –up of patients with rheumatoid arthritis, using PANACLEAR MMP-3”Latex” and Hitachi LABOSPECT008
  • Author(s): Hamada E, Inaba C, Uchiyama K, Iwahara K, Maekawa M
  • Organizer: 2013 American Association of Clinical Chemistry Annual Meeting
  • Place of Presentation: Houston USA
• [Presentation] Analytical evaluation of soluble IL2- Receptor, ACTH, GH Measurement by Automated Immunoassay System ”IMMULITE 2000XPi”
  • Author(s): Hamada E, Noji K, Maekawa M
  • Organizer: 2013 American Association of Clinical Chemistry Annual Meeting
  • Place of Presentation: Houston USA
• [Presentation] Evaluation of PANACLEAR MMP-3”Latex”a reagent for Hitachi LABOSPECT008
  • Author(s): Hamada E, Maekawa M
  • Organizer: 20th IFCC-EFLM European Congress of Clinical Chemistry and Laboratory Medicine
  • Place of Presentation: Milan Italy
• [Presentation] エピジェネティクスと臨床検査
  • Author(s): 前川真人
  • Organizer: 第53回日本臨床化学会年次学術集会
  • Place of Presentation: 徳島市
• [Presentation] 電気泳動技術の臨床的意義
  • Author(s): 前川真人
  • Organizer: 第64回日本電気泳動学会総会
  • Place of Presentation: 仙台市
• [Book] 臨床検査データブックLABDATA2015-2016 (2015)
  • Author(s): 青沼和隆、青野敏博、秋山雄次、浅川明弘、阿部好文、天谷雅行、新井祐子、荒木拓也、荒木靖人、安藤雄一、安東由喜雄、飯高 誠、飯塚 誠、伊賀立二、五十嵐 隆、伊苅裕二、池森敦子、石井 彰、石井恵子、前川真人
  • Total Pages: 1059
  • Publisher: 医学書院
• [Book] 臨床検査ガイド2015改訂版 (2015)
  • Author(s): 福井次矢、斧 康雄、三嶋理晃、島田和幸、深澤 浩、奥新和也、小池和彦、廣田衛久、下瀬川 徹、五十嵐雅彦、丹治泰仁、野村 隆、三橋知明、工藤 工、伊藤 充、黒川峰夫、南谷泰仁、鈴木勝也、竹内 勤、前川真人
  • Total Pages: 1154
  • Publisher: 文光堂
• [Book] 引いて調べる先天代謝異常症 (2014)
  • Author(s): 青山友佳、市本景子、伊藤哲哉、伊藤 康、碓井ひろみ、大竹 明、大橋十哉、小川絢子、小国弘量、小沢 浩、折居建治、梶 俊策、加藤淳二、窪田 満、呉 繁夫、顧 艶紅、古賀靖敏、小須賀基道、児玉浩子、前川真人
  • Total Pages: 175
  • Publisher: 診断と治療社
• [Book] パーフェクトガイド検査値事典第2版 (2014)
  • Author(s): 高木 康、〆谷直人、野村文夫、澤部祐司、吉田俊彦、清宮正徳、梅村啓史、戸塚 実、日野田裕治、杉浦哲郎、高橋延行、橋本直明、光井 洋、池田 均、中川勇人、高橋伯夫、渡辺 毅、浅井 淳、飯野和美、前川真人
  • Total Pages: 707
  • Publisher: 総合医学社