| Project/Area Number |
24390146
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Laboratory medicine
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| Research Institution | Kyoto Prefectural University of Medicine |
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Principal Investigator |
NAKAYA TAKAAKI 京都府立医科大学, 医学(系)研究科(研究院), 教授 (80271633)
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| Co-Investigator(Kenkyū-buntansha) |
KAIHATSU Kunihiro 大阪大学, 産業科学研究所, 特任准教授 (70419464)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥18,720,000 (Direct Cost: ¥14,400,000、Indirect Cost: ¥4,320,000)
Fiscal Year 2014: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2013: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2012: ¥7,930,000 (Direct Cost: ¥6,100,000、Indirect Cost: ¥1,830,000)
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| Keywords | 迅速診断 / デバイス / インフルエンザウイルス / ペプチド核酸 / ウイルス検出 / マイクロ流体システム / ウイルス / クロマトデバイス / オンチップ / 感染症 |
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| Outline of Final Research Achievements |
In this study, we developed a novel chromatography kit for the subtype-specific detection of influenza A viruses. Azobenzene-tethered hairpin-type peptide nucleic acid (Tail-clamp PNA-AZO) possessing a subtype-specific complementary sequence against human influenza A virus, H1-, H3-, and avian H7-subtype, was synthesized and attached to a chromatography strip. The sensitivity of the chromatography was approximately 1.0×104 ffu/kit for H1N1 and H3N2, and 5.0×105 ffu/kit for H7N7, respectively. The PNA-chromatography specifically detected each influenza A virus tested, although it slightly reacted with other subtypes.
Even though further improvement is necessary to use it clinically as a rapid diagnostic kit, this system could be applied for the sequence-specific detection of influenza viruses, including drug-resistant viruses as well as highly-pathogenic avian influenza viruses.
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