An evaluation of environmental pollutant exposure using cell survival signaling and development of simple detection system

• Project/Area Number: 24390159
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Hygiene
• Research Institution: University of Occupational and Environmental Health, Japan
• Principal Investigator: YOSHIDA Yasuhiro 産業医科大学, 医学部, 准教授 (10309958)
• Co-Investigator(Kenkyū-buntansha): KAWAMOTO Toshihiro 産業医科大学, 医学部, 教授 (60177748)
• Research Collaborator: MORITA Kentaro ; SONG Yuan ; NAKANISHI Tsukasa ; HE Cuiying
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥13,130,000 (Direct Cost: ¥10,100,000、Indirect Cost: ¥3,030,000); Fiscal Year 2014: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2013: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2012: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
• Keywords: 環境汚染物質 / 粒子サイズ / NF-κB / LPS / 粒子状物質 / NF-kB / アポトーシス / オートファジー

Outline of Final Research Achievements

Exposure to elevated concentrations of particulate matter (PM), air pollution has been implicated as a risk factor for our health. These pollutants include a mineral, a chemical substance and microbes such as bacteria. In this communication, we showed that administration of PM or exposure of chemicals to mice modified immune responses. Furthermore, different size of particles showed different modification in immune responses. It will open the door for new assessment for detection of exposure of air pollutants.

Research Products

• [Journal Article] Lung inflammation by fungus, Bjerkandera adusta isolated from Asian sand dust (ASD) aerosol and enhancement of ovalbumin -induced lung eosinophilia by ASD and the fungus in mice. (2014)
  • Author(s): Liu B, Ichinose T, He M, Kobayashi F, Maki T, Yoshida S, Yoshida Y, Arashidani K, Takano H, Nishikawa M, Sun G, Shibamoto T.
  • Journal Title: Allergy Asthma Clin Immunol. Volume: 10 Issue: 1 Pages: 30-30
  • DOI: 10.1186/1710-1492-10-10
  • Peer Reviewed / Open Access
• [Journal Article] Enhancement of OVA-induced murine lung eosinophilia by co-exposure to contamination levels of LPS in Asian sand dust and heated dust. (2014)
  • Author(s): Ren Y, Ichinose T, He M, Song Y, Yoshida Y, Yoshida S, Nishikawa M, Takano H, Sun G, Shibamoto T.
  • Journal Title: Allergy Asthma Clin Immunol Volume: 10 Issue: 1 Pages: 30-30
  • DOI: 10.1186/1710-1492-10-30
  • Peer Reviewed / Open Access
• [Journal Article] Silica-carrying particulate matter enhances Bjerkandera adusta-induced murine lung eosinophilia. (2014)
  • Author(s): He M, Ichinose T, Liu B, Song Y, Yoshida Y, Kobayashi F, Maki T, Yoshida S, Nishikawa M, Takano H, Sun G.
  • Journal Title: Environ Toxicol Volume: - Issue: 1 Pages: 1-10
  • DOI: 10.1002/tox.22025
  • Peer Reviewed / Open Access
• [Journal Article] Aggravation of ovalbumin-induced murine asthma by co-exposure to desert-dust and organic chemicals: an animal model study. (2014)
  • Author(s): Ren Y, Ichinose T, He M, Arashidani K, Yoshida Y, Yoshida S, Nishikawa M, Takano H, Sun G, Shibamoto T
  • Journal Title: Environ Health. Volume: 13 Pages: 83-83
  • Peer Reviewed / Open Access
• [Journal Article] A rapid method for estimating the levels of urinary thiobarbituric Acid reactive substances for environmental epidemiologic survey (2014)
  • Author(s): Kil HN, Eom SY, Park JD, Kawamoto T, Kim YD, Kim H
  • Journal Title: Toxicol Res Volume: 1 Issue: 1 Pages: 7-11
  • DOI: 10.5487/tr.2014.30.1.007
  • Peer Reviewed
• [Journal Article] Asian sand dust causes subacute peripheral immune modification with NF-κB activation. (2014)
  • Author(s): Song Y, Ichinose T, Morita K, Nakanishi T, Kanazawa T, Yoshida Y
  • Journal Title: Environ Toxicol. Volume: in press Issue: 5 Pages: 549-558
  • DOI: 10.1002/tox.21931
  • Peer Reviewed
• [Journal Article] Effects of two Asian sand dusts transported from the dust source regions of Inner Mongolia and northeast China on murine lung eosinophilia. (2013)
  • Author(s): He M, Ichinose T, Song Y, Yoshida Y, Arashidani K, Yoshida S, Liu B, Nishikawa M, Takano H, Sun G.:
  • Journal Title: Toxicol Appl Pharmacol. Volume: 272 Issue: 3 Pages: 647-655
  • DOI: 10.1016/j.taap.2013.07.010
  • Peer Reviewed
• [Journal Article] The effect of ethanol on the formation of N^<2-> ethylidene-dG adducts in mice: implications for alcohol-related carcinogenicity of the oral cavity and esophagus (2012)
  • Author(s): Yu H-S, Oyama T, Matsuda T, Isse T, Yamaguchi T, Tanaka M, Tsuji M, Kawamoto T
  • Journal Title: Biomarkers Volume: 17 Issue: 3 Pages: 269-274
  • DOI: 10.3109/1354750x.2012.666675
  • Peer Reviewed
• [Presentation] 黄砂とPM2.5免疫応答の差異と微生物成分の関与に関する研究 (2015)
  • Author(s): 吉田 安宏, 宋 媛, 何 翠頴, 嵐谷 奎一, 市瀬 孝道
  • Organizer: 第９回大気バイオエアロゾルシンポジウム
  • Place of Presentation: 別府ビーコンプラザ（大分県・別府）
  • Year and Date: 2015-01-28 – 2015-01-29
• [Presentation] Particulate matter influenced immune responses through TLR4 in vivo (2014)
  • Author(s): Song Yuan, Ichinose Takamichi, Morita Kentaro, Nakanishi Tsukasa, He Cuiying, Kanazawa Tamotsu, Yoshida Yasuhiro
  • Organizer: 第43回日本免疫学会総会
  • Place of Presentation: 京都国際会議場（京都府・京都）
  • Year and Date: 2014-12-10 – 2014-12-12
• [Presentation] Asian sand dust causes acute lung inflammation and subacute peripheral events. (2014)
  • Author(s): Yasuhiro Yoshida, Yuan Song, Takamichi Ichinose
  • Organizer: World asthma
  • Place of Presentation: New York（USA）
  • Year and Date: 2014-04-26 – 2014-04-29
• [Presentation] Z36 induces cell death on human T-cell leukemia cell lines (2012)
  • Author(s): 中西 司, 吉田 安宏, ら
  • Organizer: 第35回日本分子生物学会
  • Place of Presentation: 福岡国際会議場(福岡)
  • Year and Date: 2012-12-13
• [Presentation] 化学物質曝露による特異的抗体の検出に関する研究 (2012)
  • Author(s): 余旭 勝, 吉田 安宏, 川本 俊弘, ら
  • Organizer: 第30回産業医科大学学大会
  • Place of Presentation: ラマツィーニホール(北九州)
  • Year and Date: 2012-10-20
• [Presentation] TLR4 is involved in Asian sand dust-induced peripheral inflammation.
  • Author(s): Song, Y., Ichinose, T., Kanazawa, T. and Yoshida, Y.
  • Organizer: 第36回日本免疫学会総会
  • Place of Presentation: 千葉 (幕張メッセ）
• [Presentation] Asian sand dust induces autophagic markers and activates NF-κB in immune cells
  • Author(s): Song, Y., Ichinose, T., Kanazawa, T. and Yoshida, Y.
  • Organizer: 100th American association of Immunology annual meeting
  • Place of Presentation: USA, Honolulu (Hawaii Convension center)
• [Presentation] Intratracheally administration of Asian sand dust activates NF-κB in immune cells
  • Author(s): Song, Y., Ichinose, T., Kanazawa, T. and Yoshida, Y
  • Organizer: 15th International congress of Immunology
  • Place of Presentation: Itary, Miran (Mico-Milano Congressi)