Epithelial regeneration by transplantation of cultured intestinal stem cells
Project/Area Number |
24390186
|
Research Category |
Grant-in-Aid for Scientific Research (B)
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Allocation Type | Partial Multi-year Fund |
Section | 一般 |
Research Field |
Gastroenterology
|
Research Institution | Tokyo Medical and Dental University |
Principal Investigator |
NAKAMURA Tetsuya 東京医科歯科大学, 医歯(薬)学総合研究科, 寄附講座教授 (70265809)
|
Project Period (FY) |
2012-04-01 – 2015-03-31
|
Project Status |
Completed (Fiscal Year 2014)
|
Budget Amount *help |
¥18,200,000 (Direct Cost: ¥14,000,000、Indirect Cost: ¥4,200,000)
Fiscal Year 2014: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2013: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2012: ¥9,620,000 (Direct Cost: ¥7,400,000、Indirect Cost: ¥2,220,000)
|
Keywords | 消化管再生医療 / 腸管上皮幹細胞 / 幹細胞移植 / 消化管上皮 / 組織幹細胞 / 細胞移植 / 大腸炎治療 / 消化管上 |
Outline of Final Research Achievements |
To develop stem cell therapy for human intestinal diseases, it is essential to assess whether intestinal stem cells in culture retain their tissue regeneration capabilities. During the term of this grant support, we developed a new mouse recipient model of colonic mucosal injury, which allows us to assess the time-course changes and behaviors of transplanted stem cells. By combining the cell transplantation technique developed in our laboratory, we investigated whether cultured intestinal stem cells are capable of regenerating epithelia in vivo, and how they behave when transplanted onto the colon. We found that stem cells of the adult colon and small intestine and also epithelial progenitors of the fetal small intestine are all capable of reconstituting self-renewing epithelia when transplanted. This study provides evidence that cultured intestinal stem cells could be a source for cell therapy of intestinal diseases.
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Report
(4 results)
Research Products
(29 results)
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[Journal Article] Small intestinal stem cell identity is maintained with functional Paneth cells in heterotopically grafted epithelium onto the colon2014
Author(s)
Fukuda M, Mizutani T, Mochizuki W, Matsumoto T, Nozaki K, Sakamaki Y, Ichinose S, Okada Y, Tanaka T, Watanabe M, Nakamura T
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Journal Title
Genes & Development
Volume: 28(16)
Issue: 16
Pages: 1752-1757
DOI
Related Report
Peer Reviewed / Open Access / Acknowledgement Compliant
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[Journal Article] Functional engraftment of colon epithelium expanded in vitro from a single adult Lgr5+stem cell2012
Author(s)
Yui S, Nakamura T, Sato T, Nemoto Y, Mizutani T, Zheng X, Ichinose S, Nagaishi T, Okamoto R, Tsuchiya K, Clevers H, and Watanabe M
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Journal Title
Nature Medicine
Volume: 18
Issue: 4
Pages: 618-623
DOI
Related Report
Peer Reviewed
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