Elucidation of the pathogenesis of kidney disease via epigenetic responses against hypoxia
| Project/Area Number |
24390213
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Kidney internal medicine
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| Research Institution | The University of Tokyo |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
INAGI Reiko 東京大学, 医学部附属病院, 特任准教授 (50232509)
WADA Takehiko 東京大学, 医学部附属病院, 講師 (90447409)
TANAKA Tetsuhiro 東京大学, 医学部附属病院, 病院講師 (90508079)
OHSE Takamoto 東京大学, 医学部附属病院, その他 (10568447)
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| Co-Investigator(Renkei-kenkyūsha) |
KODAMA Tatsuhiko 東京大学, 先端科学技術センター, 教授 (90170266)
ABURATANI Hiroyuki 東京大学, 先端科学技術センター, 教授 (10202657)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥18,070,000 (Direct Cost: ¥13,900,000、Indirect Cost: ¥4,170,000)
Fiscal Year 2014: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2013: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
Fiscal Year 2012: ¥8,580,000 (Direct Cost: ¥6,600,000、Indirect Cost: ¥1,980,000)
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| Keywords | 低酸素 / HIF / エピジェネティックス / エピゲノム / ヒストン修飾 / 慢性腎臓病 / 腎細胞癌 / エピジェネティクス |
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| Outline of Final Research Achievements |
To elucidate a pathogenic role of epigenetic changes in the kidney in vivo, we tried to optimize experimental conditions to perform ChIP-seq of organs. We attempted to perform PAT-ChIP utilizing laser capture microdissection. However, we could not obtain RNA with the quality high enough for ChIP analysis. We finally decided to perform ChIP of the whole kidney with expectation that the results should reflect change of the tubules, which comprise most of the kidney. We employed a kidney fibrosis model that develops long after transient acute kidney injury by ischemia/reperfusion. We found that fibrotic conditions alter by treatment of compounds that regulate histone modifications.
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Report
(4 results)
Research Products
(57 results)
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[Journal Article] SIRT1 maintains actin cytoskeleton by deacetylation of cortactin in injured podocytes.2015
Author(s)
Motonishi S, Nangaku M, Wada T, Ishimoto Y, Ohse T, Matsusaka T, Kubota N, Shimizu A, Kadowaki T, Tobe K, Inagi R.
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Journal Title
J Am Soc Nephrol
Volume: x
Related Report
Peer Reviewed / Acknowledgement Compliant
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[Journal Article] Glyoxalase I reduces glycative and oxidative stress and prevents age-related endothelial dysfunction through modulation of endothelial nitric oxide synthase phosphorylation.2014
Author(s)
Jo-Watanabe A, Ohse T, Nishimatsu H, Takahashi M, Ikeda Y, Wada T, Shirakawa J, Nagai R, Miyata T, Nagano T, Hirata Y, Inagi R, Nangaku M.
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Journal Title
Aging Cell
Volume: Epub ahead of print
Issue: 3
Pages: 519-28
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Cross-enhancement of ANGPTL4 transcription by HIF1 alpha and PPAR beta/delta is the result of the conformational proximity of two response elements.2014
Author(s)
Inoue T, Kohro T, Tanaka T, Kanki Y, Li G, Poh HM, Mimura I, Kobayashi M, Taguchi A, Maejima T, Suehiro JI, Sugiyama A, Kaneki K, Aruga H, Dong S, Stevens JF, Yamamoto S, Tsutsumi S, Fujita T, Ruan X, Aburatani H, Nangaku M, Ruan Y, Kodama T, Wada Y.
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Journal Title
Genome Biology
Volume: 15
Issue: 4
Pages: R63-R63
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Sperm-associated antigen 4, a novel hypoxia-inducible factor 1 target, regulates eytokinesis, and its expression correlates with the prognosis of renal cell earcinoma.2013
Author(s)
Shoji K, Mimura I, Murayama T, Wada T, Kume H, Goto A, Ohse T, Tanaka T, Inagi R, van der Hoorn FA, Manabe I, Homma Y, Fukayama M, Sakurai T, Hasegawa T, Aburatani H, Kodama T, Nangaku M.
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Journal Title
American Journal of Pathology
Volume: 182
Issue: 6
Pages: 2191-2203
DOI
Related Report
Peer Reviewed
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[Presentation] Glyoxalase I Prevents Age-Related Endothelial Dysfunction through Modulation of eNOS Phosphorylation.
Author(s)
Airi Jo, Takamoto Ohse, Hiroaki Nishimatsu, Masao Takahashi, Satoshi Unuma, Takehiko Wada, Yoichiro Ikeda, Toshio Miyata, Yasunobu Hirata, Reiko Inagi, Masaomi Nangaku.
Organizer
The 46th Annual Meeting of the American Society of Nephrology
Place of Presentation
Atlanta, USA
Related Report
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[Presentation] The Mechanism of ANGPTL4 Induction, a Mediator of Nephrotic Syndrome, through Its Chromatin Conformational Change.
Author(s)
Tsuyoshi Inoue, Imari Mimura, Takahide Kohro, Toshiya Tanaka, Hiroyuki Aburatani, Tatsuhiko Kodama, Youichiro Wada, Masaomi Nangaku
Organizer
The 46th Annual Meeting of the American Society of Nephrology
Place of Presentation
Atlanta, USA
Related Report
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