| Budget Amount *help |
¥17,940,000 (Direct Cost: ¥13,800,000、Indirect Cost: ¥4,140,000)
Fiscal Year 2014: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2013: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2012: ¥8,190,000 (Direct Cost: ¥6,300,000、Indirect Cost: ¥1,890,000)
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| Outline of Final Research Achievements |
NFATc1 is a key transcription factor that regulates osteoclast differentiation through transactivation of a wide variety of target genes in response to RANKL stimulation. However, a little is known about how NFATc1 regulates and selects functionally distinct target genes differentially during differentiation stage. We performed RNA-sequence analysis and LC-MS/MS analysis and identified the novel NFATc1 binding proteins including WHSC1 and its post-translational modification. RNA-seq revealed that genes related with signal transductions such as TRAF1 and non-coding RNA were induced at early-time point, whereas the induction of NFATc1 and genes associated with osteoclastic functions was observed at maturation stage. Thus, our research suggest that WHSC1 associates with NFATc1 and then regulates RANKL-dependent signaling pathway through modulating certain target genes of NFATc1, possibly linking to the coordination of differentiation and functional regulation of osteoclasts.
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