Elucidation of Bone Epigenetics with NFATc1 Complex Analysis using Cross-linking Method.
| Project/Area Number |
24390239
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Endocrinology
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| Research Institution | Toho University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
TANAKA Tomoaki 千葉大学, 大学院医学研究院, 准教授 (50447299)
YOSHIDA Tomohiko 千葉大学, 総合安全衛生管理機構, 助教 (20422222)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥17,940,000 (Direct Cost: ¥13,800,000、Indirect Cost: ¥4,140,000)
Fiscal Year 2014: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2013: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2012: ¥8,190,000 (Direct Cost: ¥6,300,000、Indirect Cost: ¥1,890,000)
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| Keywords | 遺伝子 / ゲノム / 発現制御 / エピジェネティクス / 発生・分化 / 骨リモデリング / 骨粗鬆症 / エピジェネクス |
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| Outline of Final Research Achievements |
NFATc1 is a key transcription factor that regulates osteoclast differentiation through transactivation of a wide variety of target genes in response to RANKL stimulation. However, a little is known about how NFATc1 regulates and selects functionally distinct target genes differentially during differentiation stage. We performed RNA-sequence analysis and LC-MS/MS analysis and identified the novel NFATc1 binding proteins including WHSC1 and its post-translational modification. RNA-seq revealed that genes related with signal transductions such as TRAF1 and non-coding RNA were induced at early-time point, whereas the induction of NFATc1 and genes associated with osteoclastic functions was observed at maturation stage. Thus, our research suggest that WHSC1 associates with NFATc1 and then regulates RANKL-dependent signaling pathway through modulating certain target genes of NFATc1, possibly linking to the coordination of differentiation and functional regulation of osteoclasts.
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Report
(4 results)
Research Products
(69 results)
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[Journal Article] Methylation of Gata3 at Arg261 regulates transactivation of the Il5 gene in T helper 2 cells2015
Author(s)
Hosokawa, H., Kato, M., Tohyama, H., Tamaki, Y., Endo, Y., Kimura, M. Y., Tumes, D. J., Motohashi, S., Matsumoto, M., Nakayama, K. I., Tanaka, T., and Nakayama, T.
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Journal Title
J Biol Chem
Volume: in press
Issue: 21
Pages: 13095-13103
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Gata3/Ruvbl2 complex regulates T helper 2 cell proliferation via repression of Cdkn2c expression.2013
Author(s)
Hosokawa H, Tanaka T, Kato M, Tohyama H, Hanazawa A, Tamaki Y, Hirahara K, Sakikawa I, Morita A, Nagira M, Suzuki Y, Nakayama T.
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Journal Title
Proc Natl Acad Sci U S A.
Volume: 110(46)
Issue: 46
Pages: 18626-18631
DOI
Related Report
Peer Reviewed
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[Journal Article] Detection of adrenal veins on selective retrograde CT adrenal venography in comparison with digital subtraction angiography in subjects with established diagnosis of one-sided adrenal aldosterone- producing tumor confirmed by adrenal vein sampling, histopathology and clinical course.2013
Author(s)
Higashide T, Funabashi N, Yanaka T, Inoue K, Kazama T, Motoori K, Nagano H, Takaoka H, Uehara M, Nishiyama A, Kato H, Yokote K, Kobayashi Y, Uno T.
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Journal Title
Int J Cardiol
Volume: 168(4)
Issue: 4
Pages: 3254-3258
DOI
Related Report
Peer Reviewed
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[Journal Article] Utility of 320 slice mapping CT for adrenal vein sampling in subjects suspected of having primary-aldosteronism compared with digital -subtraction-angiography and selective retrograde CT adrenal venography.2013
Author(s)
Higashide T, Funabashi N, Tanaka T, Inoue K, Kazama T, Motoori K, Nagano H, Takaoka H, Uehara M, Nishiyama A, Kato H, Yokote K, Kobayashi Y, Uno T.
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Journal Title
Int J Cardiol
Volume: 168(3)
Issue: 3
Pages: 3033-3034
DOI
Related Report
Peer Reviewed
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[Presentation] WHSC1 associates with NFATc1 and regulates RANKL-dependent osteoclast differentiation2015
Author(s)
Nakayama, A., Yoshida, T., Nagano, H., Suzuki, S., Koide, H., Suzuki, Y., Sugano, S., Yokote, K., Tatsuno, I., Tanaka, T
Organizer
The Endocrine society’s 97th annual meeting & expo
Place of Presentation
San Diego U.S.A
Year and Date
2015-03-05
Related Report
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