Development of gene therapy for refractory non-Hodgkin lymphoma using T-cells expressing a chimeric antigen receptor

• Project/Area Number: 24390247
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Hematology
• Research Institution: Jichi Medical University
• Principal Investigator: OZAWA keiya 自治医科大学, 医学部, 客員教授 (30137707)
• Co-Investigator(Kenkyū-buntansha): TSUKAHARA Tomonori 自治医科大学, 医学部, 講師 (10362120) ; UCHIBORI Ryousuke 自治医科大学, 医学部, 助教 (20458285) ; OHMINE Ken 自治医科大学, 医学部, 講師 (90316521)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥17,940,000 (Direct Cost: ¥13,800,000、Indirect Cost: ¥4,140,000); Fiscal Year 2014: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2013: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000); Fiscal Year 2012: ¥6,760,000 (Direct Cost: ¥5,200,000、Indirect Cost: ¥1,560,000)
• Keywords: 遺伝子治療 / 免疫療法 / 細胞療法 / キメラ抗原受容体 / Tリンパ球 / 造血器腫瘍 / 血液腫瘍学

Outline of Final Research Achievements

Gene therapy for refractory B-cell non-Hodgkin lymphoma using T-cells expressing a chimeric antigen receptor (CAR) was studied as follows: (1) To improve the anti-tumor activity, T-cells were transduced with CD19-targeted CAR and IL-21 genes. However, the therapeutic effect was not enhanced by IL-21 in tumor-bearing mice. (2) For the safety, we developed a switch promoter, which stimulates transgene expression in CAR T-cells upon interaction with target cells. We demonstrated that this system works through CAR-dependent signaling in CAR T-cells in vitro and in tumor-bearing mice. (3) We manufactured CD19-CAR T-cells from patient-derived PBMCs by a closed cell-processing system, and confirmed that these T-cells can kill B lymphoma cells in vitro. (4) A clinical protocol (CD19-CAR T-cell therapy for B-cell lymphoma) was approved by IRB and the Ministry of Health, Labour and Welfare. Enrollment started in December 2014.

Research Products

• [Journal Article] Corticospinal Tract Development and Spinal Cord Innervation Differ between Cervical and Lumbar Targets. (2015)
  • Author(s): Kamiyama T, Kameda H, Murabe N, Fukuda S, Yoshioka N, Mizukami H, Ozawa K, Sakurai M.
  • Journal Title: J Neurosci. Volume: 35 Issue: 3 Pages: 1181-91
  • DOI: 10.1523/jneurosci.2842-13.2015
  • Peer Reviewed
• [Journal Article] Comparative analyses of adeno-associated viral vector serotypes 1, 2, 5, 8 and 9 in marmoset, mouse and macaque cerebral cortex. (2015)
  • Author(s): Watakabe A, Ohtsuka M, Kinoshita M, Takaji M, Isa K, Mizukami H, Ozawa K, Isa T, and Yamamori T.
  • Journal Title: Neurosci. Res. Volume: 93 Pages: 144-157
  • DOI: 10.1016/j.neures.2014.09.002
  • Peer Reviewed / Open Access
• [Journal Article] Distribution of serum erythropoietin levels in Japanese patients with myelodysplastic syndromes. (2015)
  • Author(s): Suzuki T, Oh I, Ohmine K, Meguro A, Mori M, Fujiwara S, Yamamoto C, Nagai T, Ozawa K.
  • Journal Title: Int J Hematol. Volume: 101 Issue: 1 Pages: 32-36
  • DOI: 10.1007/s12185-014-1699-3
  • Peer Reviewed / Open Access
• [Journal Article] Central retinal vein occlusion caused by hyperviscosity syndrome in a young patient with Sjögren's syndrome and MALT lymphoma. (2015)
  • Author(s): Yoshida A, Watanabe M, Ohmine K, Kawashima H.
  • Journal Title: Int Ophthalmol. Volume: 35 Issue: 3 Pages: 429-32
  • DOI: 10.1007/s10792-015-0056-8
  • Peer Reviewed
• [Journal Article] The Tol2 transposon system mediates the genetic engineering of T-cells with CD19-specific chimeric antigen receptors for B-cell malignancies (2015)
  • Author(s): Tsukahara, T., Iwase, N., Kawakami, K., Iwasaki, M., Yamamoto, C., Ohmine, K., Uchibori, R., Teruya, T., Ido, H., Saga, Y., Urabe, M., Mizukami, H., Kume, A., Nakamura, M., Brentjens, R., and Ozawa, K.
  • Journal Title: Gene Therapy Volume: 22 Issue: 2 Pages: 209-215
  • DOI: 10.1038/gt.2014.104
  • Peer Reviewed / Open Access
• [Journal Article] Long-term outcome of patients with acquired chronic pure red cell aplasia (PRCA) following immunosuppressive therapy: a final report of the nationwide cohort study in 2004/2006 by the Japan PRCA collaborative study group. (2015)
  • Author(s): Hirokawa M, Sawada K, Fujishima N, Teramura M, Bessho M, Dan K, Tsurumi H, Nakao S, Urabe A, Fujisawa S, Yonemura Y, Kawano F, Oshimi K, Sugimoto K, Matsuda A, Karasawa M, Arai A, Komatsu N, Harigae H, Omine M, Ozawa K, Kurokawa M; PRCA Collaborative Study Group.
  • Journal Title: Br J Haematol. Volume: in press Issue: 6 Pages: 879-886
  • DOI: 10.1111/bjh.13376
  • Peer Reviewed / Open Access
• [Journal Article] Glial dysfunction in the mouse habenula causes depressive-like behaviors and sleep disturbance. (2014)
  • Author(s): Cui W, Mizukami H, Yanagisawa M, Aida T, Nomura M, Isomura Y, Takayanagi R, Ozawa K, Tanaka K, and Aizawa H.
  • Journal Title: J. Neurosci. Volume: 34 Issue: 49 Pages: 16273-16285
  • DOI: 10.1523/jneurosci.1465-14.2014
  • Peer Reviewed
• [Journal Article] Manufacturing and characterization of a recombinant adeno-associated virus type 8 reference standard material. (2014)
  • Author(s): Ayuso E, et al
  • Journal Title: Hum. Gene Ther. Volume: 25 Issue: 11 Pages: 977-987
  • DOI: 10.1089/hum.2014.057
  • Peer Reviewed
• [Journal Article] Leukemia cells directly phagocytose blood cells in AML-associated hemophagocytic lymphohistiocytosis: A case report and review of the literature. (2014)
  • Author(s): Hatano K, Nagai T, Matsuyama T, Sakaguchi , Fujiwara S.I, Oh I, Muroi K, and Ozawa K.
  • Journal Title: Acta Haematol. Volume: 133 Issue: 1 Pages: 98-100
  • DOI: 10.1159/000360840
  • Peer Reviewed / Open Access
• [Journal Article] The bone marrow hematopoietic microenvironment is impaired in iron-overloaded mice. (2014)
  • Author(s): Okabe H, Suzuki T, Uehara E, Ueda M, Nagai T, Ozawa K.
  • Journal Title: Eur J Haematol. Volume: in Press Issue: 2 Pages: 118-128
  • DOI: 10.1111/ejh.12309
  • Peer Reviewed
• [Journal Article] Production of functional coagulation factor VIII from iPSCs using a lentiviral vector (2014)
  • Author(s): Kashiwakura Y, Ohmori T, Mimuro J, Madoiwa S, Inoue M, Hasegawa M, Ozawa K, and Sakata Y.
  • Journal Title: Haemophilia Volume: 20 Issue: 1
  • DOI: 10.1111/hae.12311
  • Peer Reviewed
• [Journal Article] Novel anti-tumor mechanism of galanin receptor type 2 in head and neck squamous cell carcinoma cells (2014)
  • Author(s): Uehara T, Kanazawa T, Mizukami H, Uchibori R, Tsukahara T, Urabe M, Kume A, Misawa K, Carey T, Suzuki M, Ichimura K, Ozawa K
  • Journal Title: Cancer Science Volume: 105 Issue: 1 Pages: 72-80
  • DOI: 10.1111/cas.12315
  • Peer Reviewed / Open Access
• [Journal Article] The prevalence of neutralizing antibodies against adeno-associated virus capsids is reduced in young Japanese individuals. (2014)
  • Author(s): Mimuro J, Mizukami H, Shima M, Matsushita T, Taki M, Muto S, Higasa S, Sakai M, Ohmori T, Madoiwa S, Ozawa K, and Sakata Y.
  • Journal Title: J. Med. Virol. Volume: 86 Issue: 11 Pages: 1990-1997
  • DOI: 10.1002/jmv.23818
  • Peer Reviewed / Open Access
• [Journal Article] Mechanisms of resistance to azacitidine in human leukemia cell lines. (2014)
  • Author(s): Sripayap P, Nagai T, Uesawa M, Kobayashi H, Tsukahara T, Ohmine K, Muroi K, Ozawa K.
  • Journal Title: Exp Hematol. Volume: 42 Issue: 4 Pages: 294-306
  • DOI: 10.1016/j.exphem.2013.12.004
  • Peer Reviewed
• [Journal Article] Cancer gene therapy using mesenchymal stem cells. (2014)
  • Author(s): Uchibori R, Tsukahara T, Ohmine K, Ozawa K.
  • Journal Title: Int J Hematol. Volume: 99 Issue: 4 Pages: 377-82
  • DOI: 10.1007/s12185-014-1537-7
  • Peer Reviewed
• [Journal Article] Intrathecal administration of high-titer cytomegalovirus immunoglobulin for cytomegalovirus meningitis. (2014)
  • Author(s): Fujiwara S, Muroi K, Tatara R, Ohmine K, Matsuyama T, Mori M, Nagai T, Ozawa K.
  • Journal Title: Case Rep Hematol. Volume: 2014 Pages: 1-3
  • DOI: 10.1155/2014/272458
  • Peer Reviewed
• [Journal Article] Effect of Intra-Medullar and Intra-Venous Infusions of Mesenchymal Stem Cells on Cell Engraftment by In-Vivo Cell Tracking and Osteoinductivity in Rabbit Long Bones: A Pilot Study. (2014)
  • Author(s): Ishihara A, Ohmine K, Weisbrode SE, Bertone AL.
  • Journal Title: Orthop Muscular Syst. Volume: 3 Issue: 03 Pages: 1-5
  • DOI: 10.4172/2161-0533.1000172
  • Peer Reviewed / Open Access
• [Journal Article] IL-21 is not necessary for anti-lymphoma effects of T cells expressing second-generation CD19-specific chimeric antigen receptors in a xenograft mouse model. (2014)
  • Author(s): Yamamoto C, Tsukahara T, Omine K, Teruya T, Ido H, Uchibori R, Urabe M, Mizukami H, Kume A, Nakamura M, Ozawa K.
  • Journal Title: Jichi Medical University Journal Volume: 36 Pages: 23-31
  • NAID: 120006408261
  • Peer Reviewed
• [Journal Article] Suppression of lymph node and lung metastases of endometrial cancer by muscle-mediated expression of soluble VEGF receptor-3 (2013)
  • Author(s): Takahashi K, Mizukami H, Saga Y, Takei Y, Urabe M, Kume A, Machida S, Fujiwara H, Suzuki M, Ozawa K
  • Journal Title: Cancer Science Volume: 104 Issue: 8 Pages: 1107-1111
  • DOI: 10.1111/cas.12184
  • Peer Reviewed
• [Journal Article] CD19 target-engineered T-cells accumulate at tumor lesions in human B-cell lymphoma xenograft mouse models (2013)
  • Author(s): Tsukahara T, Ohmine K, Yamamoto C, Uchibori R, Ido H, Teruya T, Urabe M, Mizukami H, Kume A, Nakamura M, Mineno J, Takesako K, Riviere I, Sadelain M, Brentjens R, Ozawa K
  • Journal Title: Biochemical and Biophysical Research Communications Volume: 438 Issue: 1 Pages: 84-89
  • DOI: 10.1016/j.bbrc.2013.07.030
  • Peer Reviewed
• [Journal Article] Unrelated allogeneic bone marrow-derived mesenchymal stem cells for steroid-refractory acute graft-versus-host disease: a phase I/II study. (2013)
  • Author(s): Muroi K, Miyamura K, Ohashi K, Murata M, Eto T, Kobayashi N, Taniguchi S, Imamura M, Ando K, Kato S, Mori T, Teshima T, Mori M, Ozawa K.
  • Journal Title: Int J Hematol. Volume: 98 Issue: 2 Pages: 206-213
  • DOI: 10.1007/s12185-013-1399-4
  • NAID: 10031194736
  • Peer Reviewed
• [Journal Article] Overexpression of factor VII ameliorates bleeding diathesis of factor VIII-deficient mice with inhibitors. (2013)
  • Author(s): Yasumoto A, Madoiwa S, Kashiwakura Y, Ishiwata A, Ohmori T, Mizukami H. Ozawa K, Sakata Y, and Mimuro J.
  • Journal Title: Thromb. Res. Volume: 131 Issue: 5 Pages: 444-449
  • DOI: 10.1016/j.thromres.2013.03.007
  • Peer Reviewed
• [Journal Article] Minimizing the inhibitory effect of neutralizing antibody for efficient gene expression in the liver with adeno-associated virus 8 vectors. (2013)
  • Author(s): Mimuro J, Mizukami H, Hishikawa S, Ikemoto T, Ishiwata A, Sakata A, Ohmori T, Madoiwa S, Ono F, Ozawa K, and Sakata Y
  • Journal Title: Mol Ther Volume: 21 Issue: 2 Pages: 318-323
  • DOI: 10.1038/mt.2012.258
  • Peer Reviewed
• [Journal Article] NF-κB activity regulates mesenchymal stem cell accumulation at tumor sites. (2013)
  • Author(s): Uchibori R, Tsukahara T, Mizuguchi H, Saga Y, Urabe M, Mizukami H, Kume A, Ozawa K
  • Journal Title: Cancer Research Volume: 73 Issue: 1 Pages: 364-372
  • DOI: 10.1158/0008-5472.can-12-0088
  • Peer Reviewed
• [Journal Article] Downregulation of indoleamine-2,3-dioxygenase in cervical cancer cells suppresses tumor growth by promoting natural killer cell accumulation. (2012)
  • Author(s): Sato, N.
  • Journal Title: Oncol Rep. Volume: 28 Issue: 5 Pages: 1574-1578
  • DOI: 10.3892/or.2012.1984
  • Peer Reviewed
• [Journal Article] Intra-articular injection of mesenchymal stem cells expressing coagulation factorameliorates hemophilic arthropathy in factor VIII-deficient mice. (2012)
  • Author(s): Kashiwakura, Y
  • Journal Title: J Thromb Haemost. Volume: 10(9) Issue: 9 Pages: 1802-1813
  • DOI: 10.1111/j.1538-7836.2012.04851.x
  • Peer Reviewed
• [Journal Article] Cetuximab inhibits the growth of mucinous ovarian carcinoma tumor cells lacking KRAS gene mutations. (2012)
  • Author(s): Sato, N.
  • Journal Title: Oncol Rep. Volume: 27 Pages: 1336-1340
  • DOI: 10.3892/or.2012.1626
  • Peer Reviewed
• [Journal Article] Interleukin-10 expression induced by adeno-associated virus vector suppresses proteinuria in Zucker obese rats (2012)
  • Author(s): Ogura M, Urabe M, Akimoto T, Onishi A, Ito C, Ito T, Tsukahara T, Mizukami H, Kume A, Muto S, Kusano E, and Ozawa K.
  • Journal Title: Gene Ther Volume: 19巻 Issue: 5 Pages: 476-82
  • DOI: 10.1038/gt.2011.183
  • Peer Reviewed
• [Journal Article] Recovery of neurogenic amines in phenylketonuria mice after liver-targeted gene therapy (2012)
  • Author(s): Yagi, H., Sanechika, S., Ichinose, H., Sumi-Ichinose, C., Mizukami, H., Urabe, M., Ozawa, K., and Kume, A.
  • Journal Title: Neuroreport Volume: 23 Issue: 1 Pages: 30-34
  • DOI: 10.1097/wnr.0b013e32834e3a87
  • Peer Reviewed
• [Presentation] CD19特異的キメラ抗原受容体発現Tリンパ球を用いた難治性B細胞性悪性リンパ腫に対する遺伝子治療 (2014)
  • Author(s): 大嶺 謙
  • Organizer: IMSUT-CGCTキックオフ・シンポジウム2014
  • Place of Presentation: 東京
  • Year and Date: 2014-11-21
  • Invited
• [Presentation] Analysis of function and response specificity of a switch promoter driven by activation signals from a CD19-targeted chimeric antigen receptor. (2014)
  • Author(s): Uchibori R, Takeshi T, Tsukahara T, Ohmine K, Riviere I, Brentjens RJ, Sadelain M, Mineno J, Takesako K, Ozawa K.
  • Organizer: The 5th Meeting of Asian Cellular Therapy Organization.
  • Place of Presentation: 大阪
  • Year and Date: 2014-11-11
• [Presentation] Tol2 transposon-based gene transfer of CD19-specific chimeric antigen receptors into human T-cells. (2014)
  • Author(s): Tsukahara T, Iwase N, Yamamoto C, Ohmine K, Uchibori R, Teruya T, Ido H, Urabe M, Mizukami H, Kume A, Nakamura M, Brentjens R, Kawakami K and Ozawa K.
  • Organizer: 第76回日本血液学会学術集会
  • Place of Presentation: 大阪
  • Year and Date: 2014-11-02
• [Presentation] Activation signals from CAR permit NFAT-controlled inducible expression of transgenes in PBMCs. (2014)
  • Author(s): Uchibori R, Ninomiya S, Tsukahara T, Ido H, Teruya T, Ohmine K, Urabe M, Mizukami H, Kume A, Riviere I, Sadelain M, Brentjens RJ, Mineno J, Ozawa K.
  • Organizer: 第76回日本血液学会学術集会
  • Place of Presentation: 大阪
  • Year and Date: 2014-11-02
• [Presentation] フィラデルフィア染色体陽性急性白血病に対するPSL併用Dasatinib療法の効果と安全性に関する報告 (2014)
  • Author(s): 翁 家国, 多々良 礼音, 藤原 慎一郎, 大嶺 謙, 鈴木 隆浩, 永井 正, 室井 一男, 小澤 敬也
  • Organizer: 第76回日本血液学会学術集会
  • Place of Presentation: 大阪
  • Year and Date: 2014-10-31
• [Presentation] Activation signals from chimeric antigen receptors permit NFAT-controlled inducible expression of transgene in PBMCs. (2014)
  • Author(s): Uchibori R, Ninomiya S, Tsukahara T, Ido H, Teruya T, Ohmine K, Urabe M, Mizukami H, Kume A, Mineno J, Ozawa K.
  • Organizer: 第73回日本癌学会学術総会
  • Place of Presentation: 横浜
  • Year and Date: 2014-09-26
• [Presentation] Generation of T-cells expressing a CD19-specific CAR by the Tol2 transposon system. (2014)
  • Author(s): Tsukahara T, Ohmine K, Uchibori R, Urabe M, Mizukami H, Kume A, Nakamura M, Ozawa K.
  • Organizer: 第73回日本癌学会学術総会
  • Place of Presentation: 横浜
  • Year and Date: 2014-09-25
• [Presentation] キメラ抗原受容体からの抗原刺激特異的な遺伝子発現誘導系の開発. (2014)
  • Author(s): 内堀 亮介, 二宮 空暢, 照屋 武志, 塚原 智典, 大嶺 謙, 峰野 純一, 竹迫 一任, 小澤 敬也.
  • Organizer: 第6回血液疾患免疫療法研究会
  • Place of Presentation: 京都
  • Year and Date: 2014-09-06
• [Presentation] Tol2 transposon-mediated gene transfer of CD19-CAR to primary human T-cells for the treatment of B-cell malignancies. (2014)
  • Author(s): Tsukahara T, Iwase N, Yamamoto C, Ohmine K, Uchibori R, Takeshi T, Urabe M, Mizukami H, Kume A, Nakamura M, Brentjens R J, Kawakami K, Ozawa K.
  • Organizer: 第20回日本遺伝子治療学会
  • Place of Presentation: 東京
  • Year and Date: 2014-08-08
• [Presentation] The NFAT response elements in the IL-2 gene promoter permit visualization and quantification of activation status in CAR-expressing PBMCs. (2014)
  • Author(s): Uchibori R, Ninomiya S, Tsukahara T, Ido H, Teruya T, Ohmine K, Urabe M, Mizukami H, Kume A, Riviere I, Sadelain M, Brentjens RJ, Mineno J, Ozawa K.
  • Organizer: 第20回日本遺伝子治療学会
  • Place of Presentation: 東京
  • Year and Date: 2014-08-08
• [Presentation] B細胞リンパ腫に合併した腫瘍随伴性天疱瘡と考えた1例 (2014)
  • Author(s): 坂口 優, 宮本 有希子, 佐藤 篤子, 藤田 有理香, 小宮根 真弓, 村田 哲, 大槻 マミ太郎, 佐藤 一也, 大嶺 謙, 小澤 敬也
  • Organizer: 第113回 日本皮膚科学会総会
  • Place of Presentation: 京都
  • Year and Date: 2014-05-30
• [Presentation] Development of switch promoters that is activated to induce exogenous gene expression upon the recognition of CD19 by chimeric antigen receptors. (2014)
  • Author(s): Uchibori R, Ninomiya S, Tsukahara T, Ido H, Teruya T, Ohmine K, Urabe M, Mizukami H, Kume A, Riviere I, Sadelain M, Brentjens RJ, Mineno J, Ozawa K.
  • Organizer: 17th Annual Meeting of the American Society of Gene & Cell Therapy.
  • Place of Presentation: Washington, D.C.
  • Year and Date: 2014-05-22
• [Presentation] 細胞療法の現状と将来 キメラ抗原受容体発現Tリンパ球を用いたB細胞性腫瘍に対する遺伝子治療 (2014)
  • Author(s): 大嶺 謙
  • Organizer: 第62回日本輸血・細胞治療学会総会
  • Place of Presentation: 奈良
  • Year and Date: 2014-05-17
  • Invited
• [Presentation] Validation of anti-tumor activity mediated by T-lymphocytes with chimeric antigen receptor targeting CD19 for B cellymphoma (2012)
  • Author(s): 塚原智典
  • Organizer: 第74回日本血液学会学術集会
  • Place of Presentation: 国立京都国際会館(京都)
  • Year and Date: 2012-10-20
• [Presentation] Bリンパ腫に対する抗CD19キメラ抗原受容体発現T細胞の抗腫瘍効果 (2012)
  • Author(s): 塚原智典
  • Organizer: 第71回日本癌学会学術総会
  • Place of Presentation: ロイトン札幌(札幌)
  • Year and Date: 2012-09-20
• [Presentation] Evaluation of anti-tumor effects mediated by engineered Tlymphocytes expressing a CD19 specific CAR for B cell lymphoma (2012)
  • Author(s): Tsukahara, T.
  • Organizer: The 15th Annual Meeeting of The American Society of Gene and Cell Therapy
  • Place of Presentation: Pennsylvania Convention Center (USA)
  • Year and Date: 2012-05-19
• [Presentation] Tumor targeting and anti-tumor activity mediated by T-lymphocytes with an anti-CD19 CAR in human B-cell lymphoma models.
  • Author(s): Tsukahara T, Ohmine K, Uchibori R, Ido H, Urabe M, Mizukami H, Kume A, Nakamura M, Riviere I, Sadelain M, Brentjens R, Ozawa K.
  • Organizer: 第18回日本遺伝子治療学会
  • Place of Presentation: 岡山市
• [Presentation] Anti-tumor activity of CD19-specific chimeric antigen receptor-modified T-cells for B cell lymphoma.
  • Author(s): Tsukahara T, Ohmine K, Uchibori R, Ido H, Urabe M, Mizukami H, Kume A, Nakamura M, Ozawa K.
  • Organizer: 第72回日本癌学会学術総会
  • Place of Presentation: 横浜市
• [Presentation] Tumor targeting using T-lymphocytes with an anti-CD19 chimeric antigen receptor for B cell lymphoma.
  • Author(s): Tsukahara T, Ohmine K, Uchibori R, Ido H, Urabe M, Mizukami H, Kume A, Nakamura M, Riviere I, Sadelain M, Brentjens R, Ozawa K.
  • Organizer: 第75回日本血液学会学術集会
  • Place of Presentation: 札幌市
• [Presentation] DEVELOPMENT OF SIN RETROVIRAL VECTORS EQUIPPED WITH SWITCH PROMOTERS DRIVEN BY ACTIVATION SIGNALS FROM THE CHIMERIC ANTIGEN RECEPTORS.
  • Author(s): Uchibori R, Ninomiya S, Tsukahara T, Ido H, Teruya T, Ohmine K, Urabe M, Mizukami H, Kume A, Riviere I, Sadelain M, Brentjens RJ, Mineno J, Ozawa K.
  • Organizer: 第18回日本遺伝子治療学会
  • Place of Presentation: 岡山市
• [Presentation] Development of switch promoters that would respond to activation signals from the chimeric antigen receptors.
  • Author(s): Uchibori R, Ninomiya S, Tsukahara T, Ido H, Teruya T, Ohmine K, Urabe M, Mizukami H, Kume A, Riviere I, Sadelain M, Brentjens RJ, Mineno J, Ozawa K.
  • Organizer: 第72回日本癌学会学術総会
  • Place of Presentation: 横浜市
• [Presentation] Development of switch promoters driven by activation signals from the chimeric antigen receptors.
  • Author(s): Uchibori R, Ninomiya S, Tsukahara T, Ohmine K, Urabe M, Mizukami H, Kume A, Mineno J, Ozawa K.
  • Organizer: 第75回日本血液学会学術集会
  • Place of Presentation: 札幌市