| Budget Amount *help |
¥16,770,000 (Direct Cost: ¥12,900,000、Indirect Cost: ¥3,870,000)
Fiscal Year 2014: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2013: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2012: ¥8,190,000 (Direct Cost: ¥6,300,000、Indirect Cost: ¥1,890,000)
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| Outline of Final Research Achievements |
Various genetic alterations have been reported in lymphoid malignancies. Frequencies of such alterations in each disease entity are low except for translocation-juncture genes. It is not an easy task to provide evidences that such genes are directly involved in lymphomagenesis. Transgenic and know-out mouse technology is limited in analyzing several genes within a relatively short period of time. In this research project, we have established the cell culture system, using feeder cells and cytokines, which can analyze several genes by retroviral vector transduction. Indeed, we could transform normal mouse B-cells to lethally malignant lymphoma by three translocation juncture genes, MYC, BCL2 and CCND1. This system could be extended to examine oncogenic ability for several other genes reported by genomic or exome sequencing. This system will further provide a good system to screen molecular targets.
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