Development of cancer stem cell-targeted therapy by study of tumor sphere formation mechanism using comprehensive and genetic approach
Project/Area Number |
24390269
|
Research Category |
Grant-in-Aid for Scientific Research (B)
|
Allocation Type | Partial Multi-year Fund |
Section | 一般 |
Research Field |
Pediatrics
|
Research Institution | Research Institute for Clinical Oncology, Saitama Cancer Center (2014) Chiba Cancer Center (Research Institute) (2012-2013) |
Principal Investigator |
KAMIJO Takehiko 埼玉県立がんセンター(臨床腫瘍研究所), その他部局等, その他 (90262708)
|
Co-Investigator(Kenkyū-buntansha) |
田尻 達郎 京都府立医科大学, 大学院, 教授 (80304806)
吉田 英生 千葉大学医学系研究科, 研究院, 教授 (60210712)
竹信 尚典 千葉県がんセンター, 研究所, 研究員 (60392247)
春田 雅之 埼玉県立がんセンター, 臨床腫瘍研究所, 研究員 (80392190)
|
Project Period (FY) |
2012-04-01 – 2015-03-31
|
Project Status |
Completed (Fiscal Year 2014)
|
Budget Amount *help |
¥17,940,000 (Direct Cost: ¥13,800,000、Indirect Cost: ¥4,140,000)
Fiscal Year 2014: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2013: ¥5,980,000 (Direct Cost: ¥4,600,000、Indirect Cost: ¥1,380,000)
Fiscal Year 2012: ¥7,670,000 (Direct Cost: ¥5,900,000、Indirect Cost: ¥1,770,000)
|
Keywords | 癌幹細胞 / 腫瘍スフェア / 神経芽腫 / 網羅的解析 / 癌 / 遺伝子 / 小児がん / 小児腫瘍学 / がん幹細胞 |
Outline of Final Research Achievements |
We performed screening of molecules which specifically expressed in neuroblastoma sphere and its high expression related to poor prognosis of neuroblastoma patients. Finally, we identified two interesting candidates by the wet/dry experiments; the candidate molecules were co-receptor molecule CXX1 and transcription factor HXX1. Knockdown of co-receptor molecule CXX1 in sphere-forming neuroblastoma cells successfully inhibited sphere formation. Further, using yeast two-hybrid screening, we identified receptor-type protein tyrosine phosphatase K (PTPRK) as a binding partner of CD133, a cancer stemness-related molecule. Silencing of PTPRK elevated the tyrosine phosphorylation of CD133, while forced expression of PTPRK reduced its phosphorylation level markedly and abrogated CD133-mediated AKT phosphorylation. The tyrosine phosphorylation of CD133, which is dephosphorylated by PTPRK, regulates AKT signaling and plays a critical role in tumorigenesis (ONCOGENE, 2015).
|
Report
(4 results)
Research Products
(46 results)
-
-
-
-
-
-
-
[Journal Article] Unique expression features of cancer-type organic anion transporting polypeptide 1B3 mRNA expression in human colon and lung cancers: potential biomarker implications.2014
Author(s)
Sun, Y., Furihata, T., Ishii, S., Nagai, M., Harada, M., Shimozato, O., Kamijo, T., Motohashi, S., Yoshino, I., Kamiichi, A., Kobayashi, K. and Chiba, K.
-
Journal Title
Clin. Transl. Med.
Volume: 3
Issue: 1
Pages: 37-37
DOI
Related Report
Peer Reviewed / Open Access
-
-
[Journal Article] RASSF1A methylation indicates a poor prognosis in hepatoblastoma patients2013
Author(s)
Honda S, Miyagi H, Suzuki H, Minato M, Haruta M, Kaneko Y, Hatanaka KC, Hiyama E, Kamijo T, Okada T, Taketomi A
-
Journal Title
Pediatric Surgery International
Volume: 29
Issue: 11
Pages: 1147-1152
DOI
Related Report
Peer Reviewed / Open Access
-
-
-
-
-
[Presentation] 神経芽腫の基礎生物学2014
Author(s)
上條岳彦
Organizer
第56回日本小児血液・がん学会学術集会
Place of Presentation
岡山コンベンションセンター(岡山市北区)
Year and Date
2014-11-28 – 2014-11-30
Related Report
Invited
-
-
-
-
-
-
-
[Presentation] 神経芽腫スフェア特異的に発現する転写因子CDX1は細胞の幹細胞性を制御する2014
Author(s)
竹信 尚典, 下里 修, 藤村 雄一, 秋田 直洋, 山口 陽子, 力石 浩志, 池田 英里子, 田川 雅敏, 中川原 章, 古関 明彦, 上條 岳彦
Organizer
第73回日本癌学会学術総会
Place of Presentation
パシフィコ横浜(神奈川県横浜市)
Year and Date
2014-09-25 – 2014-09-27
Related Report
-
-
-
-
-
-
-
-
[Presentation] Tumor Sphere Specific Transcription Factor CDX1 Regulates Stem Cell-Related Gene Expression and Aggressiveness in Neuroblastoma2014
Author(s)
Hisanori Takenobu, Osamu Shimozato, Yu-ichi Fujimura, Nobuhiro Akita, Yohko Yamaguchi, Haruka Uehara, Miyuki Waraya, Eriko Ikeda, Akira Nakagawara, Haruhiko Koseki, Takehiko Kamijo
Organizer
Advanced Neuroblastoma Research
Place of Presentation
ドイツ, ケルン市
Year and Date
2014-05-14 – 2014-05-16
Related Report
-
[Presentation] Genome-Based Sub-Classification of Neuroblastoma: A Retrospective Study by Using 573 Neuroblastoma Samples Obtained in Japan2014
Author(s)
Miki Ohira, Takehiko Kamijo, Yohko Nakamura, Tetsuya Takimoto, Atsuko Nakazawa, Junko Takita, Tomoko Iehara, Hideto Takahashi, Tatsuro Tajiri, Akira Nakagawara
Organizer
Advanced Neuroblastoma Research
Place of Presentation
ドイツ, ケルン市
Year and Date
2014-05-14 – 2014-05-16
Related Report
-
[Presentation] Subtype-Specific Whole Exome Sequencing of 101 Neuroblastomas Identi?ed Genetic Mutations Involved in Cancer-Related Pathways Particularly in Aggressive Subgroups2014
Author(s)
Yuanyuan Li, Miki Ohira, Yong Zhou, Zhibo Gao, Kenji Tatsuno, Shuichi Tsutsumi, Shogo Yamamoto, Yohko Nakamura, Takehiko Kamijo, Hiroyuki Aburatani, Akira Nakagawara
Organizer
Advanced Neuroblastoma Research
Place of Presentation
ドイツ, ケルン市
Year and Date
2014-05-14 – 2014-05-16
Related Report
-
-
-
[Presentation] RISK CLASSIFICATION OF NEUROBLASTOMA BASED ON GENOMIC PROFILES: FOR FUTURE TAILOR-MADE THERAPEUTIC STRATEGIES IN JAPAN2013
Author(s)
M. Ohira, T. Kamijo, Y. Nakamura, K. Matsumoto, M. Kumagai, A. Nakazawa, T. Takimoto, T. Fukushima, T. Tajiri, H. Ikeda, A. Nakagawara
Organizer
SIOP 2013
Place of Presentation
Hong Kong
Related Report
-
[Presentation] REVIEW OF SURGICAL TREATMENT IN PATIENTS ENROLLED IN THE PHASE II STUDY NB-HR07 FOR ADVANCED NEUROBLASTOMA - A REPORT FROM JAPAN2013
Author(s)
T. Hishiki, T. Kuroda, T. Tajiri, A. Yoneda, K. Tokiwa, T. Muraji, K. Sugito, K. Ise, Y. Kinoshita, S. Uehara, K. Matsumoto, M. Kumagai, T. Soejima, T. Takimoto, H. Takahashi, T. Kamijo, A. Makimoto, J. Hara, H. Ikeda, A. Nakagawara
Organizer
SIOP 2013
Place of Presentation
Hong Kong
Related Report
-
-
-
-
-
[Book] 最新肝癌学2014
Author(s)
上條岳彦、檜山英三
Total Pages
815
Publisher
日本臨牀社
Related Report
-
-
-
-
-
-