Analysis of human innate immunity and acquired immune mechanism during fetal period using human ES cells
Project/Area Number |
24390272
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Research Category |
Grant-in-Aid for Scientific Research (B)
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Allocation Type | Partial Multi-year Fund |
Section | 一般 |
Research Field |
Embryonic/Neonatal medicine
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Research Institution | The University of Tokyo |
Principal Investigator |
|
Co-Investigator(Kenkyū-buntansha) |
EBIHARA Yasuhiro 東京大学, 医科学研究所, 特任准教授 (40302608)
TSUJI Kohichiro 東京大学, 医科学研究所, 准教授 (50179991)
|
Project Period (FY) |
2012-04-01 – 2015-03-31
|
Project Status |
Completed (Fiscal Year 2014)
|
Budget Amount *help |
¥17,940,000 (Direct Cost: ¥13,800,000、Indirect Cost: ¥4,140,000)
Fiscal Year 2014: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2013: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2012: ¥7,670,000 (Direct Cost: ¥5,900,000、Indirect Cost: ¥1,770,000)
|
Keywords | 胎児医学 / ES細胞 / 自然免疫 |
Outline of Final Research Achievements |
We succeeded the production of human mast cells and eosinophils from hematopoietic cells derived human ES cells and established the system that reflect the generation and development of these immune cells in human embryo. It is indicated that they first develop connective tissue-type mast cells that both express chymase and tryptase at primary time of induction and then from multipotential hematopoietic progenitor cells at a comparatively later stage they develop mucosal mast cells. It is also suggested that eosinophils and basophils might be originated from common progenitor cells. We succeeded the production of human neutrophils from hematopoietic cells derived human iPS cells as well. In the analyses, they showed normal and healthy functions, compared with the one derived from iPS cells from patients of congenital neutrophilic disorders. it is expected to be a useful tool for investigations into the mechanism of the generation of innate immune system and many diseases.
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Report
(4 results)
Research Products
(39 results)
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[Journal Article] Screening of Drugs to Treat 8p11 Myeloproliferative Syndrome Using Patient-Derived Induced Pluripotent Stem Cells with Fusion Gene CEP110-FGFR12015
Author(s)
Yamamoto S, Otsu M, Matsuzaka E, Konishi C, Takagi H, Hanada S, Mochizuki S, Nakauchi H, Imai K, Tsuji K, Ebihara Y
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Journal Title
PloS one
Volume: 10
Issue: 3
Pages: e0120841-e0120841
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] In vitro expansion of CD34(+)CD38(-) cells under stimulation with hematopoietic growth factors on AGM-S3 cells in juvenile myelomonocytic leukemia.2015
Author(s)
Sakashita K, Kato I, Daifu T, Saida S, Hiramatsu H, Nishinaka Y, Ebihara Y, Ma F, Matsuda K, Saito S, Hirabayashi K, Kurata T, Uyen Lt, Nakazawa Y, Tsuji K, Heike T, Nakahata T, Koike K.
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Journal Title
Leukemia.
Volume: 29(3)
Issue: 3
Pages: 606-14
DOI
NAID
Related Report
Peer Reviewed / Open Access
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[Journal Article] Impaired hematopoietic differentiation of RUNX1-mutated induced pluripotent stem cells derived from FPD/AML patients2014
Author(s)
Sakurai M, Kunimoto H, Watanabe N, Fukuchi Y, Yuasa S, Yamazaki S, Nishimura T, Sadahira K, Fukuda K, Okano H, Nakauchi H, Morita Y, Matsumura I, Kudo K, Ito E, Ebihara Y, Tsuji K, Harada Y, Harada H, Okamoto S, Nakajima H
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Journal Title
Leukemia
Volume: 28
Issue: 12
Pages: 2344-54
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Acquired deficiency of A20 results in rapid apoptosis, systemic inflammation, and abnormal hematopoietic stem cell function.2014
Author(s)
Nagamachi A, Nakata Y, Ueda T, Yamasaki N, Ebihara Y, Tsuji K, Honda Zi, Takubo K, Suda T, Oda H, Inaba T, Honda H
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Journal Title
PLos One
Volume: 9
Issue: 1
Pages: 1-10
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Unusual extramedullary relapse after haploidentical bone marrow transplantation in a patient with acute lymphoblastic leukemia.2013
Author(s)
Ebihara Y, Yamamoto S, Mochizuki S, Tsukada M, Taya Y, Sato A, Kawakita T, Kato S, Ooi J, Takahashi S, Tojo A, Tsuji K.
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Journal Title
J Blood Disorders Transf
Volume: 4
Issue: 05
Pages: 155-155
DOI
Related Report
Peer Reviewed
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