Comprehensive analysis of the effect of antipsychotics on DNA methylation in brain

• Project/Area Number: 24390279
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Psychiatric science
• Research Institution: The University of Tokyo
• Principal Investigator: IWAMOTO Kazuya 東京大学, 医学部附属病院, 特任准教授 (40342753)
• Co-Investigator(Renkei-kenkyūsha): BUNDO Miki 東京大学, 医学部附属病院, 特任助教 (00597221)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥17,940,000 (Direct Cost: ¥13,800,000、Indirect Cost: ¥4,140,000); Fiscal Year 2014: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000); Fiscal Year 2013: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000); Fiscal Year 2012: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
• Keywords: エピジェネティクス / 抗精神病薬 / DNAメチル化 / ゲノム / エピゲノム

Outline of Final Research Achievements

Epigenetic status such as DNA methylation and histone protein modification is believed to be involved in the pathophyshiology of neuropsychiatric disorders. However, impact of various treatment drugs on epigenetic status remain unclear. In this project, I systematically examined the effect of antipsychotics on DNA methylation using neuroblastoma cell line.

Research Products

• [Journal Article] Effects of quetiapine on DNA methylation in neuroblastoma cells. (2014)
  • Author(s): Sugawara H, Bundo M, Asai T, Sunaga F, Ueda J, Ishigooka J, Kasai K, Kato T, Iwamoto K.
  • Journal Title: Progress in Neuro-Psychopharmacology and Biological Psychiatry Volume: 56C Pages: 117-121
  • DOI: 10.1016/j.pnpbp.2014.08.010
  • Peer Reviewed
• [Journal Article] Comprehensive DNA methylation analysis of human neuroblastoma cells treated with blonanserin. (2014)
  • Author(s): Murata Y, Nishioka M, Bundo M, Sunaga F, Kasai K, Iwamoto K.
  • Journal Title: Neuroscience Letters Volume: 563 Pages: 123-128
  • DOI: 10.1016/j.neulet.2014.01.038
  • Peer Reviewed
• [Journal Article] Comprehensive DNA methylation and hydroxymethylation analysis in the human brain and its implication in mental disorders. (2014)
  • Author(s): Kato T, Iwamoto K
  • Journal Title: Neuropharmacology Volume: 80 Pages: 133-139
  • DOI: 10.1016/j.neuropharm.2013.12.019
  • Peer Reviewed
• [Journal Article] Peripheral Biomarkers Revisited: Integrative Profiling of Peripheral Samples for Psychiatric Research. (2014)
  • Author(s): Hayashi-Takagi A, Vawter MP, Iwamoto K
  • Journal Title: Biological Psychiatry Volume: 印刷中 Issue: 12 Pages: 920-8
  • DOI: 10.1016/j.biopsych.2013.09.035
  • Peer Reviewed
• [Journal Article] Neuronal cell-type specific DNA methylation patterns of the Cacna1c gene. (2013)
  • Author(s): Nishioka Masaki
  • Journal Title: International Journal of Developmental Neuroscience Volume: 31 Pages: 89-95
  • Peer Reviewed
• [Journal Article] Comprehensive DNA methylation analysis of peripheral blood cells derived from patients with first-episode schizophrenia. (2013)
  • Author(s): Nishioka M, Bundo M, Koike S, Takizawa R, Kakiuchi C, Araki T, Kasai K, Iwamoto K
  • Journal Title: J Hum Genet Volume: 58(2) Issue: 2 Pages: 91-7
  • DOI: 10.1038/jhg.2012.140
  • NAID: 10031156430
  • Peer Reviewed
• [Journal Article] A systematic evaluation of whole genome amplification of bisulfite-modified DNA. (2012)
  • Author(s): Bundo Miki, Sunaga Fumiko, Ueda Junko, Kasai Kiyoto, Kato Tadafumi, Iwamoto Kazuya.
  • Journal Title: Clinical Epigenetics Volume: 4 Issue: 1 Pages: 22-22
  • DOI: 10.1186/1868-7083-4-22
  • Peer Reviewed
• [Journal Article] DNA methylation in schizophrenia: progress and challenges of epigenetic studies. (2012)
  • Author(s): Nishioka Masaki, Bundo Miki, Kasai Kiyoto, Iwamoto Kazuya.
  • Journal Title: Genome Medicine Volume: 4 Issue: 12 Pages: 96-96
  • DOI: 10.1186/gm397
  • Peer Reviewed
• [Journal Article] Epigenetic regulation of serotonin transporter in psychiatric disorders.
  • Author(s): Sugawara Hiroko
  • Journal Title: Journal of Genetics and Genomics Volume: (印刷中)
  • Peer Reviewed
• [Presentation] Effect of mood stabilizers and quetiapine on DNA methylation in human neuroblastoma cells (2014)
  • Author(s): Sugawara Hiroko, Bundo Miki, Asai Tatsuro, Sunaga Fumiko, Ueda Junko, Ishigooka Jun, Kasai Kiyoto, Kato Tadafumi, Iwamoto Kazuya.
  • Organizer: 22nd World Congress of Psychiatric Genetics
  • Place of Presentation: Copenhagen, Denmark
  • Year and Date: 2014-10-12 – 2014-10-16
• [Presentation] 精神疾患のエピジェネティクス (2014)
  • Author(s): 岩本和也
  • Organizer: 第36 回日本生物学的精神医学会・第57 回日本神経化学会
  • Place of Presentation: 奈良文化会館(奈良県)
  • Year and Date: 2014-09-29 – 2014-10-01
  • Invited
• [Presentation] 双極性障害のエピジェネティクス～セロトニントランスポーターにおける候補遺伝子解析 (2014)
  • Author(s): 岩本和也、文東美紀、池亀天平、菅原裕子、石郷岡純、笠井清登、加藤忠史
  • Organizer: 第36 回日本生物学的精神医学会・第57 回日本神経化学会
  • Place of Presentation: 奈良文化会館(奈良県)
  • Year and Date: 2014-09-29 – 2014-10-01
  • Invited
• [Presentation] ヒト神経系細胞株におけるquetiapineのDNAメチル化への影響 (2014)
  • Author(s): 菅原裕子、文東美紀、浅井竜朗、須永史子、上田順子、石郷岡純、笠井清登、加藤忠史、岩本和也
  • Organizer: 第11回日本うつ病学会総会
  • Place of Presentation: 広島国際会議場(広島県)
  • Year and Date: 2014-07-18 – 2014-07-19
• [Presentation] Comprehensive DNA methylation analysis of human neuroblastoma cells treated with blonanserin (2014)
  • Author(s): Murata Yui, Nishioka Masaki, Bundo Miki, Sunaga Fumiko, Kasai Kiyoto, Iwamoto Kazuya
  • Organizer: 3rd HumanMethylation450 array analysis workshops
  • Place of Presentation: London, UK
  • Year and Date: 2014-05-08 – 2014-05-09
• [Presentation] 死後脳神経細胞におけるゲノム・エピゲノム解析による精神疾患の病因探索 (2013)
  • Author(s): 岩本和也
  • Organizer: 第23回日本臨床精神神経薬理学会・第43回日本神経精神薬理学会
  • Place of Presentation: 沖縄コンベンションセンター
  • Invited
• [Presentation] 精神疾患患者末梢血試料を用いたエピゲノム解析 (2013)
  • Author(s): 岩本和也
  • Organizer: 第23回日本臨床精神神経薬理学会・第43回日本神経精神薬理学会
  • Place of Presentation: 沖縄コンベンションセンター
  • Invited
• [Presentation] Epigenetic analysis of the peripheral cells in psychiatric disorders. (2013)
  • Author(s): Iwamoto Kazuya
  • Organizer: 11th World Congress of Biological Psychiatry
  • Place of Presentation: 京都
  • Invited
• [Presentation] ヒト神経系細胞株におけるquetiapineのDNAメチル化への影響 (2013)
  • Author(s): 菅原裕子
  • Organizer: 第23回日本臨床精神神経薬理学会・第43回日本神経精神薬理学会
  • Place of Presentation: 沖縄コンベンションセンター
• [Presentation] 精神疾患末梢試料を用いたエヒゲノム解析の可能性 (2012)
  • Author(s): 岩本和也
  • Organizer: 第34回日本生物学的精神医学会
  • Place of Presentation: 神戸ポートアイランド(京都)(招待講演)
  • Year and Date: 2012-09-28
• [Presentation] Neuronal epigenomics in schizophrenia (2012)
  • Author(s): Iwamoto Kazuya
  • Organizer: 第35回日本神経科学会
  • Place of Presentation: 名古屋国際会議場(愛知)(招待講演)
  • Year and Date: 2012-09-18
• [Presentation] Epigenome analysis of brains of patients with bipolar disorder. (2012)
  • Author(s): Iwamoto Kazuya
  • Organizer: 2nd Meeting of East Asian Bipolar Forum
  • Place of Presentation: 九州大学(福岡)(招待講演)
  • Year and Date: 2012-09-07
• [Presentation] 双極性障害および統合失調症患者神経細胞核におけるエヒゲノム解析 (2012)
  • Author(s): 岩本和也
  • Organizer: 第6回エヒジェネティクス研究会年会
  • Place of Presentation: 一ツ橋学術総合センター(東京)
  • Year and Date: 2012-05-15
• [Presentation] Epigenomics of brains of patients with bipolar disorder and schizophrenia. (2012)
  • Author(s): Iwamoto Kazuya
  • Organizer: 3rd Shanghai International Conference of Epigenetics and Diseases
  • Place of Presentation: 上海(中国)(招待講演)
  • Year and Date: 2012-04-19
• [Remarks] 研究室web site
  • URL: http://www.molpsy.com/
• [Remarks] 分子精神医学講座Web site
  • URL: http://www.molpsy.com/
• [Remarks]
  • URL: http://www.molpsy.com/