| Budget Amount *help |
¥18,200,000 (Direct Cost: ¥14,000,000、Indirect Cost: ¥4,200,000)
Fiscal Year 2015: ¥3,640,000 (Direct Cost: ¥2,800,000、Indirect Cost: ¥840,000)
Fiscal Year 2014: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2013: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2012: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
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| Outline of Final Research Achievements |
The purpose of this study is to elucidate the molecular mechanism of direct effect by VEGF to breast cancer cells. In this study, VEGF signal into MB-231 cells was blocked by VEGF-knockout and soluble NRP1 expression. Experiments using these cells showed that MB-231 cells are directly stimulated by VEGF via NRP1 not VEGFR, which contributes to spindle-like morphology formation and higher cell migration activity of breast cancer cells. According to microarray-based gene expression analysis of these cells, VEGF/NRP1 signaling modulates the expression of factor X that is negative regulator of cdc42. In summary, VEGF changes morphology of MB-231 cells and stimulates motility of MB-231 cells by VEGF/NRP1/factor X/cdc42 signaling pathway.
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