Identification of pancreatic cancer stem cell lineage during pancreatic tumorigenesis
Project/Area Number |
24390305
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Research Category |
Grant-in-Aid for Scientific Research (B)
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Allocation Type | Partial Multi-year Fund |
Section | 一般 |
Research Field |
General surgery
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Research Institution | Yokohama City University |
Principal Investigator |
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Co-Investigator(Kenkyū-buntansha) |
TANIGUCHI Hideki 横浜市立大学, 医学研究科, 教授 (70292555)
TAKEBE Takanori 横浜市立大学, 医学部, 准教授 (20612625)
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Project Period (FY) |
2012-04-01 – 2015-03-31
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Project Status |
Completed (Fiscal Year 2014)
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Budget Amount *help |
¥18,590,000 (Direct Cost: ¥14,300,000、Indirect Cost: ¥4,290,000)
Fiscal Year 2014: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2013: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2012: ¥8,580,000 (Direct Cost: ¥6,600,000、Indirect Cost: ¥1,980,000)
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Keywords | 癌 / 細胞・組織 / 膵癌幹細胞 / 膵癌 / 癌幹細胞 / 転移 / がん幹細胞 / 膵臓 |
Outline of Final Research Achievements |
Pancreatic cancer tissues were harvested from different carcinogenesis stages of pancreatic model mice. The cells were separated by cell sorter using a specific antibody that recognizes a number of surface antigens known as a cancer stem cell marker. The separated cells were examined either have a capability as cancer stem cells. Cancer stem cells collected from mice with progressed cancer were found to have metastatic potential in addition to the tumor forming potency. Furthermore, pancreatic cancer stem cells with tumor -forming ability that was separated from the carcinogenic early pancreatic cancer tumor tissue does not have a metastatic potential, but the metastatic potential succeed planting cells of pancreatic cancer xenografts, which is formed from this carcinogenesis early cancer stem cells in a xenograft It revealed to exacerbate into cells having .
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Report
(4 results)
Research Products
(52 results)
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[Journal Article] Polycomb group protein Ezh2 regulates hepatic progenitor cell proliferation and differentiation in murine embryonic liver.2014
Author(s)
Koike H, Ouchi R, Ueno Y, Nakata S, Obana Y, Sekine K, Zheng YW, Takebe T, Isono K, Koseki H, Taniguchi H.
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Journal Title
PLoS One.
Volume: 9
Issue: 8
Pages: 0-2
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Efficient hepatic differentiation of human induced pluripotent stem cells in a three-dimensional microscale culture.2014
Author(s)
Zhang RR, Takebe T, Miyazaki L, Takayama M, Koike H, Kimura M, Enomura M, Zheng YW, Sekine K, Taniguchi H.
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Journal Title
Methods Mol Biol.
Volume: 1210
Pages: 131-141
DOI
ISBN
9781493914340, 9781493914357
Related Report
Peer Reviewed
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[Journal Article] The evidence of sophisticatedly heterogeneous population of human umbilical vein endothelial cells2014
Author(s)
Zheng YW, Nie YZ, Tsuchida T, Zhang RR, Aoki K, Sekine K, Ogawa M, Takebe T, Ueno Y, Sakakibara H, Hirahara F, Taniguchi H
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Journal Title
Transplant Proc
Volume: 46
Issue: 4
Pages: 1251-3
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] The CD133+CD44+ Precancerous Subpopulation of Oval Cells is a Therapeutic Target for Hepatocellular Carcinoma2014
Author(s)
Zheng YW, Tsuchida T, Shimao T, Li B, Takebe T, Zhang RR, Sakurai Y, Ueno Y, Sekine K, Ishibashi N, Imajima M, Tanaka T, and Taniguchi H
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Journal Title
Stem Cells Dev
Volume: (In press)
Issue: 18
Pages: 2237-2249
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] Ring1B Promotes Hepatic Stem/Progenitor Cell Expansion via Simultaneous Suppression of Cdkn1a and Cdkn2a.2014
Author(s)
Koike H, Ueno Y, Naito T, Shiina T, Nakata S, Ouchi R, Obana Y, Sekine K, Zheng YW, Takebe T, Isono K, Koseki H, Taniguchi H
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Journal Title
Hepatology
Volume: 印刷中
Issue: 1
Pages: 323-333
DOI
Related Report
Peer Reviewed / Open Access
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[Journal Article] The CD133+CD44+ Precancerous Subpopulation of Oval Cells is a Therapeutic Target for Hepatocellular Carcinoma2014
Author(s)
Zheng YW, Tsuchida T, Shimao T, Li B, Takebe T, Zhang RR, Sakurai Y, Ueno Y, Sekine K, Ishibashi N, Imajima M, Tanaka T, Taniguchi H.
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Journal Title
Stem Cells Dev.
Volume: in press
Related Report
Peer Reviewed
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[Journal Article] Vascularized and functional human liver from an iPSC-derived organ bud transplant.2013
Author(s)
Takanori Takebe*, Keisuke Sekine, Masahiro Enomura, Hiroyuki Koike, Ran-Ran Zhang, Yasuharu Ueno, Yun-Wen Zheng, Naoto Koike, Shinsuke Aoyama, Yasuhisa Adachi, Hideki Taniguch*(*: Correspondence)
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Journal Title
Nature
Volume: 499
Issue: 7459
Pages: 481-484
DOI
NAID
Related Report
Peer Reviewed
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[Journal Article] Vitamin D receptor in osteoblasts is a negative regulator of bone mass control2013
Author(s)
Yamamoto Y, Yoshizawa T, Fukuda T, Shirode-Fukuda Y, Yu T, Sekine K, Sato T, Kawano H, Aihara K, Naka michi Y, Watanabe T, Shindo M, Inoue K, Inoue E, Tsuji N, Hoshino M, Karsenty G, Metzger D, Chambon P, Kato S, Imai Y.
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Journal Title
Endocrinology.
Volume: 154
Issue: 3
Pages: 1008-20
DOI
Related Report
Peer Reviewed
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[Journal Article] Inhibition of Stabilin-2 elevates circulating hyaluronic acid levels and prevents tumor metastasis2012
Author(s)
Hirose, Y., Saijou, E., Sugan,o Y., Takeshita, F., Nishimura, S., Nonaka, H., Chen, Y.R., Sekine, K., Kido, T., Nakamura, T., Kato, S., Kanke, T., Nakamura, K., Nagai, R., Ochiya, T., Miyajima, A.
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Journal Title
Proc.Natl.Acad.Sci.USA
Volume: 109
Issue: 11
Pages: 4263-4268
DOI
Related Report
Peer Reviewed
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[Presentation] Targeted proteomic absolute quantification on transporters of human pancreatic cancer cells with gemcitabine-resistance.2013
Author(s)
Ueno Y, Hoshino S, Tsuchida N, Nakata S, Uchida Y, Sekine K, Zheng YW, Kurata M, Morinaga S, Miyagi Y, Yokose T, Endo I, Terasaki T, Hirano H, Taniguchi H
Organizer
HUPO 12th annual world congress.
Place of Presentation
パシフィコ横浜(神奈川)
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[Presentation] 多能性幹細胞を用いた機能的なヒト臓器の創出2012
Author(s)
武部貴則, 関根圭輔, 江野村允宏, 小池博之, 張冉冉, 木村昌樹, 魚返拓則, 吉澤絵美, 川又史佳, 上野康晴, 鄭允文, 小池直人, 谷旦英樹
Organizer
第39回日本臓器保存生物医学会学術集会
Place of Presentation
コラッセふくしま(福島県)
Related Report
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