| Project/Area Number |
24390316
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (B)
|
| Allocation Type | Partial Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Digestive surgery
|
|---|
| Research Institution | University of the Ryukyus (2014)
Clinical research Center, Chiba-East National Hospital, National Hospital Organization (2013)
Okayama University (2012) |
|---|
Principal Investigator |
NOGUCHI Hirofumi 琉球大学, 医学(系)研究科(研究院), 教授 (50378733)
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
SAITOH Issei 新潟大学, 医歯学系, 准教授 (90404540)
藤原 俊義 岡山大学, 医歯(薬)学総合研究科, 教授 (00304303)
|
|---|
| Project Period (FY) |
2012-04-01 – 2015-03-31
|
| Project Status |
Completed (Fiscal Year 2014)
|
| Budget Amount *help |
¥18,330,000 (Direct Cost: ¥14,100,000、Indirect Cost: ¥4,230,000)
Fiscal Year 2014: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2013: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2012: ¥7,800,000 (Direct Cost: ¥6,000,000、Indirect Cost: ¥1,800,000)
|
|---|
| Keywords | 膵島移植 / 膵島分離 / JNK抑制剤 / 新規臓器保存液 / 膵島純化 / 連続比重勾配 / 連続密度勾配 / JNK抑制ペプチド / 新規臓器保存液（HN-1溶液） / 連続比重・密度勾配による純化 / 臓器保存液 |
|---|
| Outline of Final Research Achievements |
We previously established a new islet isolation protocol(3rd generation) based on the Ricordi method. It includes pancreatic ductal protection, a MK preservation solution, the two-layer method of pancreas preservation, a new islet purification solution (Iodixanol-based solution), supplemental purification, and cold preservation of isolated islets. In this study, we established 4th generation of islet isolation protocol, including HN-1 preservation solution, ductal injection with JNK inhibitor, and controll-density and osmolality solution for islet purification. Establishment of this method enables us to make diabetic patients insulin independent using islets not only from 2 or 3 pancreata of NHBDs, but also from a pancreas from a brain-dead donor.
|
