| Budget Amount *help |
¥18,330,000 (Direct Cost: ¥14,100,000、Indirect Cost: ¥4,230,000)
Fiscal Year 2014: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2013: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2012: ¥7,280,000 (Direct Cost: ¥5,600,000、Indirect Cost: ¥1,680,000)
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| Outline of Final Research Achievements |
The purpose of this study is to develop YB-1 silencing miRNA/ decoy gene therapy against intractable solid tumors to regulate tumor angiogenesis. Block/homo-polyplex micelles, which encapsulate YB-1 miRNA/decoy expression plasmid with and without exportin-5 (Xpo-5) and argonaoute-2 (Ago-2) plasmids, were intraperitoneally administered in nude mice harboring disseminated pancreatic cancer. The disseminated tumors were inhibited in the YB-1 miRNA/decoy alone and the combination treatment groups compared with the controls, but there was no significant prolongation of survival between the treatment and control groups. The microvascular density was decreased in tumor tissues treated with miRNA/decoy compared with the controls. However, the localization of micelles was limitedlly detected in the peripheral area of tumor tissues. These results suggest that YB-1 silencing is promising strategy for antiangiogenesis treatment and more efficient transduction devices may resolve the issues.
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