The molecular biological analysis of spinal cord related pain and the assessment of spinal cord function using neuroimaging
| Project/Area Number |
24390351
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Orthopaedic surgery
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| Research Institution | University of Fukui |
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Principal Investigator |
UCHIDA Kenzo 福井大学, 医学部, 准教授 (60273009)
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| Co-Investigator(Kenkyū-buntansha) |
BABA Hisatoshi 福井大学, 医学部, 教授 (00165060)
NAKAJIMA Hideaki 福井大学, 医学部, 助教 (10397276)
USHIDA Takahiro 愛知医科大学, 医学部, 教授 (60304680)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥19,240,000 (Direct Cost: ¥14,800,000、Indirect Cost: ¥4,440,000)
Fiscal Year 2014: ¥1,040,000 (Direct Cost: ¥800,000、Indirect Cost: ¥240,000)
Fiscal Year 2013: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2012: ¥15,470,000 (Direct Cost: ¥11,900,000、Indirect Cost: ¥3,570,000)
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| Keywords | 脊髄損傷 / 神経障害性疼痛 / 遺伝子解析 / 画像解析 / 活性型ミクログリア |
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| Outline of Final Research Achievements |
We investigated the effects of bone marrow-derived mesenchymal stem cells (BMSC) transplantation on pain hypersensitivity in GFP-positive bone marrow-chimeric mice subjected to a contusion spinal cord injury (SCI). BMSC transplantation at day 3 post-SCI improved motor function and relieved SCI-induced hypersensitivities. The pain improvements were mediated by suppression of PKC-γ and p-CREB expression in dorsal horn neurons. BMSC transplants significantly reduced levels of mitogen activated protein kinase (MAPK) signaling (p-p38 MAPK and p-ERK1/2) in both hematogenous macrophages and resident microglia. BMSC transplants prevented hematogenous macrophages recruitment by restoration of the blood-spinal cord barrier (BSCB); reduced leakage of albumin and PDGFR-α immunoreactivity at day 7 post-SCI, which was associated with decreased levels of inflammatory cytokines. These changes is likely to play a major role in reducing pain hypersensitivity in the BMSC treated SCI animals.
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Report
(4 results)
Research Products
(27 results)
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[Presentation] 脊髄圧迫・損傷における神経生物学的可塑性と再生医学的応答2014
Author(s)
馬場久敏, 中嶋秀明, 竹浦直人, 吉田藍, 渡邉修司, 犬飼智雄, William E B Johnson, 内田研造
Organizer
第29回日本整形外科学会基礎学術集会
Place of Presentation
鹿児島市、城山観光ホテル
Year and Date
2014-10-09 – 2014-10-10
Related Report
Invited
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