| Budget Amount *help |
¥17,940,000 (Direct Cost: ¥13,800,000、Indirect Cost: ¥4,140,000)
Fiscal Year 2014: ¥7,540,000 (Direct Cost: ¥5,800,000、Indirect Cost: ¥1,740,000)
Fiscal Year 2013: ¥7,410,000 (Direct Cost: ¥5,700,000、Indirect Cost: ¥1,710,000)
Fiscal Year 2012: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
|
|---|
| Outline of Final Research Achievements |
Using an organ culture method, we succeeded in producing sperm from spermatogonial stem cells in the mouse testis tissue ex vivo for the first time in the world. Based on this achievement, we tried to induce human spermatogenesis in the same manner. We found, however, that simple application of the method to human samples did not result in spermatogenic progression. Thus, we started to examine the culture conditions in detail for its improvement. For one thing, we tried to formulate chemically-defined medium which is sufficient for mouse spermatogenesis, which now become partially possible. In addition, we thought it is also important to mimic the in vivo condition by introducing microcirculatory system. Thus, we introduced microfluidic system into our culture method, which is also effective to improve the overall culture condition. These modifications are promising for future establishment of in vitro human spermatogenesis.
|
