| Project/Area Number |
24390375
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | Tohoku University |
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Principal Investigator |
YAEGASHI Nobuo 東北大学, 医学(系)研究科(研究院), 教授 (00241597)
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| Co-Investigator(Kenkyū-buntansha) |
TOYOSHIMA Masafumi 東北大学, 大学院医学系研究科, 非常勤講師 (70451581)
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| Co-Investigator(Renkei-kenkyūsha) |
NAGASE Satoru 山形大学, 医学部, 教授 (00292326)
SUZUKI Fumihiko 東北大学, 大学院医学系研究科, 助教 (20400343)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥18,460,000 (Direct Cost: ¥14,200,000、Indirect Cost: ¥4,260,000)
Fiscal Year 2014: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Fiscal Year 2013: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000)
Fiscal Year 2012: ¥7,930,000 (Direct Cost: ¥6,100,000、Indirect Cost: ¥1,830,000)
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| Keywords | スクリーニング / 薬剤耐性 / 婦人科がん / 化学療法 / シスプラチン |
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| Outline of Final Research Achievements |
The purpose of this study is to search the molecules that can cancel the cisplatin resistance of cancer cells. We explore the molecules that show a synthetic lethal effect with a small amount of CDDP administration in a high-throughput screening using siRNA library consisting of 6650 genes. As a result, a strong expression of TIE-1 was found to be decrease the sensitivity toward cisplatin in several ovarian cancer cell lines. The relationship among TIE-1, ovarian cancer and/or drug resistance has not been reported. In order to examine the significance of TIE-1 expression in ovarian cancer patients, in silico analysis was carried out. We confirmed that TIE-1 high expression group has a significantly poor prognosis by examination of the overall survival in 1582 ovarian cancer patients. Furthermore, we confirmed that apoptosis and γH2AX expressions, a marker for DNA-double strand breaks, were significantly decreased during CDDP administration in TIE-1 over-expressed cells.
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