| Project/Area Number |
24390376
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | St. Marianna University School of Medicine |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
TAKAE Seido 聖マリアンナ医科大学, 医学部, 助教 (00621301)
YOSHIOKA Nobuhito 聖マリアンナ医科大学, 医学部, 助教 (10468928)
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| Project Period (FY) |
2012-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥18,070,000 (Direct Cost: ¥13,900,000、Indirect Cost: ¥4,170,000)
Fiscal Year 2014: ¥6,760,000 (Direct Cost: ¥5,200,000、Indirect Cost: ¥1,560,000)
Fiscal Year 2013: ¥6,630,000 (Direct Cost: ¥5,100,000、Indirect Cost: ¥1,530,000)
Fiscal Year 2012: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
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| Keywords | 原始卵胞 / 生殖補助医療 |
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| Outline of Final Research Achievements |
In patients with primary ovarian insufficiency and submenopausal women, it is difficult to conceive using their own eggs due to lack of follicle growth. Recently, we succeeded to develop an approach to activate dormant primordial follicles by a combination of PTEN inhibitor and PI3K activator. Currently, donor egg program is only effective method to treat these patients. Thus, we aimed to apply this method to regenerate production of mature eggs in these patients. Our goal is to establish a new infertility treatment to allow these patients to conceive using their own eggs. In this study, we optimized the culture method for activation of human primordial follicles, identification of optimal site of ovary transplantation and factors that facilitate survival of grafted ovaries, identification of promoting factors of early stage of human follicles and elucidation of regulation if early follicle growth, and discovered a protocol to improve implantation rate of transferred embryos.
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