Analysis of placental barrier functions for intra uterine viral infections
| Project/Area Number |
24390382
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Obstetrics and gynecology
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| Research Institution | Nihon University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
USHIJIMA Hiroshi 日本大学, 医学部, 客員教授 (10091068)
HONDA Mitsuo 日本大学, 医学部, 客員教授 (20117378)
AIZAWA Shihoko 日本大学, 医学部, 准教授 (30513858)
泉 泰之 日本大学, 医学部, 研究員 (50459872)
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| Project Period (FY) |
2012-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥18,720,000 (Direct Cost: ¥14,400,000、Indirect Cost: ¥4,320,000)
Fiscal Year 2015: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000)
Fiscal Year 2014: ¥3,250,000 (Direct Cost: ¥2,500,000、Indirect Cost: ¥750,000)
Fiscal Year 2013: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Fiscal Year 2012: ¥11,050,000 (Direct Cost: ¥8,500,000、Indirect Cost: ¥2,550,000)
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| Keywords | 母子感染 / 胎盤関門 / 風疹 / インフルエンザ / HIV / 上皮間葉移行 / 風疹ウイルス / VPｘ / マイクロビオーム / 垂直感染 / 歯周病 / 経胎盤感染 / 妊娠高血圧症候群 / マクロライド系抗菌薬 |
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| Outline of Final Research Achievements |
In order to analyze the mechanisms of intrauterine viral transmission, viral susceptibility of trophoblastic cells, yolk sac cells, immortalized trophoblasts and endothelial cells in various differentiation stages were examined. Trophoblasts and endothelial cells were highly resistant to rubella viruses cloned from both clinical isolates and vaccine strains while endothelial cells were easily and persistently infected. Influenza A viruses replicates in immortalized human early trophoblasts and induce apoptosis but their release was highly restricted. HIV replicated in syncytial trophoblasts but do not replicates in cellular and invasive trophoblasts.
Interestingly, TLR signals induces endothelial mesenchymal transition (EMT)of trophoblastic layers and enable viral entry into villous mesenchyma and subsequently fetal circulations.
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Report
(5 results)
Research Products
(40 results)
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[Journal Article] Cosavirus (family Picornaviridae) in pigs in Thailand and Japan2016
Author(s)
Okitsu S, Khamrin P, Hanaoka N, Thongprachum A, Takanashi S, Fujimoto T, Mizuguchi M, Shimizu H, Hayakawa S, Maneekarn N, Ushijima H
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Journal Title
Arch Virol
Volume: 161
Issue: 1
Pages: 159-163
DOI
Related Report
Peer Reviewed / Int'l Joint Research / Acknowledgement Compliant
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[Journal Article] Evaluation of immunochromatography tests for detection of novel GII.17 norovirus in stool samples. Khamrin P, Thongprachum A, Takanashi S, Okitsu S, Maneekarn N, Hayakawa S, Ushijima H. Euro Surveill. 2015 Jul 16;20(28).2015
Author(s)
Khamrin P, Thongprachum A, Takanashi S, Okitsu SManeekarn N, Hayakawa S, Ushijima H
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Journal Title
Euro Surveill.
Volume: 16
Pages: 20-28
Related Report
Peer Reviewed / Int'l Joint Research
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[Journal Article] Sensitive and rapid detection of campylobacter species from stools of children with diarrhea in Japan by the loop-mediated isothermal amplification method2014
Author(s)
Ushijima H, Nishimura S, Thongprachum A, Shimizu-Onda Y, Tran DN, Pham NT, Takanashi S, Dey SK, Okitsu S, Yamazaki W, Mizuguchi M, Hayakawa S.
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Journal Title
Jpn J Infect Dis.
Volume: 67
Pages: 374-378
NAID
Related Report
Peer Reviewed
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[Journal Article] Seroprevalence of chlamydophila pneumoniae in HIV-infected children in Vietnam2013
Author(s)
Trinh QD, Pham NT, Le Nguyen NT, Lam BQ, Le Phan KT, Troonq KH, Le TQ, Nguyen HT, Tang TC, Izumi Y, Komine-Aizawa S, Ushijima H, Hayakawa S
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Journal Title
J Infect Chemother
Volume: 19(3)
Issue: 3
Pages: 538-541
DOI
Related Report
Peer Reviewed
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[Journal Article] Short communication : Drug resistance mutations in the HIV type 1 protease and reverse transcriptase genes in antiretroviral-naive Vietnamese children.2013
Author(s)
Trinh QD, Pham NT, Le Nguyen NT, Lam BQ, Le Phan KT, Truong KH, Izumi Y, Komine-Aizawa S, Mizuguchi M, Ushijima H, Hayakawa S.
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Journal Title
AIDS Res Hum Retroviruses.
Volume: 28
Pages: 1305-7
Related Report
Peer Reviewed
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[Journal Article] Reemergence of new variant G3 rotavirus in Japanese pediatric patients, 2009-20112013
Author(s)
Thongprachum A, Chan-It W, Khamrin P Okitsu S, Nishimura S, Kikuta H, Yamamoto A, Sugita K, Baba T, Mizuguchi M, Maneekarn N, Hayakawa S, Ushijima H.
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Journal Title
Infect Genet Evol.
Volume: 13
Pages: 168-74
DOI
Related Report
Peer Reviewed
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