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Biological characterization of pediatric cancer stem cell, CTC, and DTC

Research Project

Project/Area Number 24390397
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypePartial Multi-year Fund
Section一般
Research Field Pediatric surgery
Research InstitutionKeio University

Principal Investigator

Kuroda Tatsuo  慶應義塾大学, 医学部, 教授 (60170130)

Co-Investigator(Kenkyū-buntansha) FUCHIMOTO Yasushi  慶應義塾大学, 医学部, 講師(非常勤) (40219077)
TAGUCHI Tomoaki  九州大学, 医学(系)研究科(研究院), 教授 (20197247)
KANO Motohiro  慶應義塾大学, 医学部, 助教 (00573403)
Co-Investigator(Renkei-kenkyūsha) SAYA Hideyuki  慶應義塾大学, 医学部, 教授 (80264282)
Research Collaborator YOSHIDA Tsuyoshi  慶應義塾大学, 医学部, 大学院生
TAKAHASHI Nobuhiro  慶應義塾大学, 医学部, 大学院生
Project Period (FY) 2012-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥18,200,000 (Direct Cost: ¥14,000,000、Indirect Cost: ¥4,200,000)
Fiscal Year 2015: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2014: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2013: ¥4,420,000 (Direct Cost: ¥3,400,000、Indirect Cost: ¥1,020,000)
Fiscal Year 2012: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000)
Keywords小児外科学 / 小児腫瘍学 / 小児がん / 神経芽細胞腫 / 腫瘍幹細胞 / 循環腫瘍細胞 / 微小転移細胞 / 横紋筋肉腫 / 肝芽腫
Outline of Final Research Achievements

Similarity between cancer stem cell and circulating tumor cell (CTC)/ disseminated tumor cell (DTC) was noticed in pediatric cancers. CD44, a stem cell marker candidate, was expressed on all cell lines and clinical tissue specimens including hepatoblastoma, neuroblastoma, rhabdomyosarcoma, and rhabdoid tumor, whereas CD44v expression was quite low. CD13 positive hepatoblastoma cell line failed to form tumors after administrated in SCID-NOD mice. Tranist downregulated the sphere formation in hepatoblastoma cell lines, and also downregulated the stem cell proliferation when administrated with CDDP, whereas did not affect the proliferation of other cell lines. Expression of stem cell candidates is modified in a different manner in each molecule even in a identical cell line. Proliferation of cancer stem cell seems to be controlled in a different mechanism in each cancer.

Report

(5 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Annual Research Report
  • 2013 Annual Research Report
  • 2012 Annual Research Report
  • Research Products

    (24 results)

All 2015 2014 2013 2012 Other

All Journal Article (20 results) (of which Peer Reviewed: 19 results) Presentation (4 results)

  • [Journal Article] Secondary cancers after a childhood cancer diagnosis: a nationwide hospital-based retrospective cohort study in Japan2015

    • Author(s)
      Ishida Y, Qiu D, Maeda M, Fujimoto J, Kigasawa H, Kobayashi R, Sato M, Okamura J, Yoshinaga S, Rikiishi T, Shichino H, Kiyotani C, Kudo K, Asami K, Hori H, Kawaguchi H, Inada H, Adachi S, Manabe A, Kuroda T
    • Journal Title

      Int J Clin Oncol

      Volume: - Issue: 3 Pages: 506-516

    • DOI

      10.1007/s10147-015-0927-z

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Application of nuclear medicine to achieve less invasive surgery for malignant solid tumors in children2014

    • Author(s)
      Hotta R, Fujimura T, Shimojima N, Nakahara T, Fuchimoto Y, Hoshino K, Morikawa Y, Matsufuji H, Kuroda T.
    • Journal Title

      Pediatr Int.

      Volume: 56 Issue: 6 Pages: 896-901

    • DOI

      10.1111/ped.12368

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 小児がん診断後の二次がん発症に関する疫学研究 15病院における後ろ向きコホート(Secondary cancers after cancer diagnosis in childhood: A hospital-based retrospective cohort study in Japan)2014

    • Author(s)
      石田 也寸志(愛媛県立中央病院 小児医療センター), 邱 冬梅, 前田 美穂, 藤本 純一郎, 気賀沢 寿人, 小林 良二, 佐藤 真穂, 岡村 純, 吉永 信治, 力石 健, 七野 浩之, 清谷 知賀子, 工藤 寿子, 浅見 恵子, 堀 浩樹, 川口 浩史, 稲田 浩子, 足立 壮一, 真部 淳, 黒田 達夫
    • Journal Title

      日本小児血液・がん学会雑誌

      Volume: 51 Pages: 261-261

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 新生児の難治性良性腫瘍の現状と展望 難治性肝血管腫2014

    • Author(s)
      黒田 達夫(慶応義塾大学 医学部小児外科)
    • Journal Title

      周産期学シンポジウム

      Volume: 32 Pages: 39-42

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 横紋筋肉腫治療後26年で発症した放射線誘発性軟骨肉腫の1例2014

    • Author(s)
      宇高 徹(慶応義塾大学 医学部整形外科), 須佐 美知郎, 中山 ロバート, 渡部 逸央, 堀内 圭輔, 星野 健, 黒田 達夫, 佐々木 文, 向井 万起男, 戸山 芳昭, 森岡 秀夫
    • Journal Title

      臨床整形外科

      Volume: 49 Pages: 723-728

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 当院での進行・再発神経芽腫に対する外科的治療の検討2014

    • Author(s)
      高橋 信博(慶応義塾大学 医学部小児外科), 富田 紘史, 石濱 秀雄, 藤村 匠, 加藤 源俊, 藤野 明浩, 星野 健, 嶋田 博之, 高橋 孝雄, 黒田 達夫
    • Journal Title

      日本小児外科学会雑誌

      Volume: 50 Pages: 645-645

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 小児悪性固形腫瘍の臨床研究グループの現状と今後 日本横紋筋肉腫研究グループ(JRSG)の展開と臨床試験の今後の課題2014

    • Author(s)
      黒田 達夫(慶応義塾大学 小児外科), 森川 康英, 池田 均, 大植 孝治, 木下 義晶, 仁尾 正記, 檜山 英三
    • Journal Title

      日本小児外科学会雑誌

      Volume: 50 Pages: 396-396

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Cetuximab promotes anticancer drug toxicity in rhabdomyosarcomas with EGFR amplification in vitro2013

    • Author(s)
      Yamamoto Y, Fukuda K, Fuchimoto Y, Matsuzaki Y, Saikawa Y, Kitagawa Y, Morikawa Y, Kuroda T
    • Journal Title

      Oncol Rep

      Volume: 30(3) Issue: 3 Pages: 1081-1086

    • DOI

      10.3892/or.2013.2588

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 若年発症のがん2013

    • Author(s)
      黒田 達夫
    • Journal Title

      臨床外科

      Volume: 68(5) Pages: 564-568

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Hepatoblastoma metastasis confined to the pulmonary artery: report of a case2013

    • Author(s)
      Izumi Y, Hoshino K, Shimojima N, Fuchimoto Y, Hayashi Y, Morikawa Y, Nomori H
    • Journal Title

      Ann Thorac Cardiovasc Surg.

      Volume: 19(3) Pages: 253-256

    • NAID

      130002148312

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 【プロが見せる手術シリーズ(3):難易度の高い肝・胆・膵・脾・横隔膜手術】 限局型先天性高インスリン血症に対する膵切除・膵空腸吻合.2013

    • Author(s)
      渡辺, 清水, 竹添, 右田, 高橋, 大野, 佐藤, 渕本, 堀川, 松岡, 北村, 岩中, 金森.
    • Journal Title

      小児外科. [解説/特集]

      Volume: 45(11) Pages: 1210-1214

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 固形腫瘍の治療の進歩 小児期の固形悪性腫瘍治療における局所治療の役割.2013

    • Author(s)
      黒田, 星野, 下島, 藤野, 金森, 渕本, 森、野坂、正木、中澤
    • Journal Title

      日本小児血液・がん学会雑誌

      Volume: 50(3) Pages: 322-326

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Hepatoblastoma Metastasis Confined to the Pulmonary Artery: Report of a Case2013

    • Author(s)
      Izumi Y, Hoshino K, Shimojima N, Fuchimoto Y, Hayashi Y, Morikawa Y, Nomori H
    • Journal Title

      Annals of Thoracic and Cardiovascular Surgery

      Volume: 19 Issue: 3 Pages: 253-256

    • DOI

      10.5761/atcs.cr.11.01848

    • NAID

      130002148312

    • ISSN
      1341-1098, 2186-1005
    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Li-Fraumeni syndrome with simultaneous osteosarcoma and liver cancer : increased expression of a CD44 variant isoform after chemotherapy2012

    • Author(s)
      Yoshida G, Fuchimoto Y, Osumi T, Shimada H, Hosaka S, Morioka H, Mukai M, Masugi Y, Sakamoto M, Kuroda T
    • Journal Title

      BMC Cancer

      Volume: 12 Issue: 1 Pages: 444-444

    • DOI

      10.1186/1471-2407-12-444

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Vincristine, actinomycin D, cyclophosphamide chemotherapy resolves Kasabach-Merritt syndrome resistant to conventional therapies2012

    • Author(s)
      Fuchimoto Y., Morikawa N., Kuroda T., Hirobe S., Kamagata S., Kumagai M., Matsuoka K., Morikawa Y.
    • Journal Title

      Pediatrics International

      Volume: 54 Pages: 285-287

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Pharmacokinetics of mizoribine in adult living donor liver transplantation2012

    • Author(s)
      Shinoda M
    • Journal Title

      Transplant Proc

      Volume: 44 Issue: 5 Pages: 1329-1335

    • DOI

      10.1016/j.transproceed.2012.01.139

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The germline TP53 mutation c.722 C>T promotes bone and liver tumorigenesis at a young age2012

    • Author(s)
      Osumi T, Miharu M, Fuchimoto Y, Morioka H, Kosaki K, Shimada H
    • Journal Title

      Pediatr Blood Cancer

      Volume: 59 Issue: 7 Pages: 1332-1333

    • DOI

      10.1002/pbc.24269

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Discontinuation of Living Donor Liver Transplantation due to Donor's Intraoperative Latex-Induced Anaphylactic Shock2012

    • Author(s)
      Shinoda M, Tanabe M, Nagao K, Kitago M, Fujisaki H, Odaira M, Kawachi S, Itano O, Obara H, Matsubara K, Shimojima N, Fuchimoto Y, Hoshino K, Amagai M, Kuroda T, Kitagawa Y
    • Journal Title

      Int Surg.

      Volume: 97 Issue: 4 Pages: 356-359

    • DOI

      10.9738/cc89.1

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Mixed chimerismによる免疫寛容誘導一大動物における前臨床研究と臨床応用2012

    • Author(s)
      渕本康史
    • Journal Title

      総説 今日の移植

      Volume: 25 Pages: 301-308

    • Related Report
      2012 Annual Research Report
  • [Journal Article] Implications of surgical intervention in the treatment of neuroblastomas : 20-year experience of a single institution2012

    • Author(s)
      Tajiri T, Souzaki R, Kinoshita Y, Koga Y, Suminoe A, Hara T, Taguchi T
    • Journal Title

      Surg Today

      Volume: 42 Pages: 220-224

    • NAID

      10030231044

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Presentation] 小児期の固形腫瘍治療における局所治療の役割2012

    • Author(s)
      黒田 達夫
    • Organizer
      第54回日本小児血液・がん学会(シンポジウム)
    • Place of Presentation
      パシフィコ横浜(神奈川県)
    • Year and Date
      2012-11-30
    • Related Report
      2012 Annual Research Report
  • [Presentation] 異なるクローンからなる神経芽腫2例から示唆された直視下生検の重要性2012

    • Author(s)
      Fuchimoto Y, Tanabe M, Hoshino K, Yamada Y, Kawachi S, Itano O, Obara H, Kawachi S, Itano O, Obara H, Shimojima N, Matsubara K, Kitagawa Y, Ohdan O, Kuroda T
    • Organizer
      American Transplant Cogress
    • Place of Presentation
      ボストン(アメリカ)
    • Year and Date
      2012-06-04
    • Related Report
      2012 Annual Research Report
  • [Presentation] 異なるクローンからなる神経芽腫2例から示唆された直視下生検の重要性2012

    • Author(s)
      狩野元宏,測本康史,加藤源俊,藤村匠,有末篤宏,高里文香,富田紘史,下島直樹,星野健,大隅朋生,嶋田博之,中澤温子,黒田達夫
    • Organizer
      第49回日本小児外科学会学術総会
    • Place of Presentation
      パシフィコ横浜(神奈川県)
    • Year and Date
      2012-05-14
    • Related Report
      2012 Annual Research Report
  • [Presentation] AHR agonistが肝芽腫細胞株HuH6に与える影響についての検討=第一報

    • Author(s)
      狩野 元宏
    • Organizer
      第55回日本小児血液がん学会
    • Place of Presentation
      ヒルトン福岡シーホーク(福岡県)
    • Related Report
      2013 Annual Research Report

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Published: 2012-04-24   Modified: 2019-07-29  

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