| Budget Amount *help |
¥18,590,000 (Direct Cost: ¥14,300,000、Indirect Cost: ¥4,290,000)
Fiscal Year 2014: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2013: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2012: ¥8,580,000 (Direct Cost: ¥6,600,000、Indirect Cost: ¥1,980,000)
|
|---|
| Outline of Final Research Achievements |
Inguinal fat pads of GFP mice and wild-type mice were cross-transplanted beneath the scalp. At 1, 2, 4, and 12 weeks post-transplantation, grafted fat was evaluated. Bone marrow of GFP mice was transplanted to wild-type mice(after irradiation) and were analyzed as well. The majority of host-derived cells detected during remodeling of grafted fat were macrophages. At 12 weeks, mature adipocytes largely were derived from adipose stem/stromal cells (ASCs) of grafts. Although vascular wall graft, vascular endothelial cells originated equally from graft and host bone marrow. ASCs of regenerated fat were an admixture of grafted, host non-bone marrow, and host bone marrow cells. Above findings underscore the importance of ASCs in the grafted fat for adipocyte regeneration. Host bone marrow and local tissues contributed substantially to capillary networks and provided new ASCs.
|
