Functional roles and potential clinical application of bone marrow-derived stem cells in tissue repair

• Project/Area Number: 24390398
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Plastic surgery
• Research Institution: The University of Tokyo
• Principal Investigator: YOSHIMURA KOTARO 東京大学, 医学部附属病院, 講師 (60210762)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥18,590,000 (Direct Cost: ¥14,300,000、Indirect Cost: ¥4,290,000); Fiscal Year 2014: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000); Fiscal Year 2013: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000); Fiscal Year 2012: ¥8,580,000 (Direct Cost: ¥6,600,000、Indirect Cost: ¥1,980,000)
• Keywords: 骨髄由来幹細胞 / 脂肪幹細胞 / 血管内皮前駆細胞 / ファイブロサイト / 創傷治癒 / モビライゼーション / 間葉系幹細胞 / マクロファージ / 骨髄移植 / 組織移植 / 幹細胞 / 血管内皮細胞 / 脂肪由来幹細胞 / 交換移植 / 組織リモデリング

Outline of Final Research Achievements

Inguinal fat pads of GFP mice and wild-type mice were cross-transplanted beneath the scalp. At 1, 2, 4, and 12 weeks post-transplantation, grafted fat was evaluated. Bone marrow of GFP mice was transplanted to wild-type mice(after irradiation) and were analyzed as well. The majority of host-derived cells detected during remodeling of grafted fat were macrophages. At 12 weeks, mature adipocytes largely were derived from adipose stem/stromal cells (ASCs) of grafts. Although vascular wall graft, vascular endothelial cells originated equally from graft and host bone marrow. ASCs of regenerated fat were an admixture of grafted, host non-bone marrow, and host bone marrow cells. Above findings underscore the importance of ASCs in the grafted fat for adipocyte regeneration. Host bone marrow and local tissues contributed substantially to capillary networks and provided new ASCs.

Research Products

• [Journal Article] Therapeutic Potential of Adipose-Derived SSEA-3-Positive Muse Cells for Treating Diabetic Skin Ulcers. (2015)
  • Author(s): Kinoshita K, Kuno S, Ishimine H, Aoi N, Mineda K, Kato H, Doi K, Kanayama K, Feng J, Mashiko T, Kurisaki A, Yoshimura K.
  • Journal Title: Stem Cells Transl Med Volume: 4 Issue: 2 Pages: 146-155
  • DOI: 10.5966/sctm.2014-0181
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Differential contributions of graft- and host-derived cells in tissue regeneration/remodeling after fat grafting. (2015)
  • Author(s): Doi K, Ogata F, Eto H, Kato H, Kuno S, Kinoshita K, Kanayama K, Feng J, Manabe I, Yoshimura K.
  • Journal Title: Plast Reconstr Surg Volume: 未定
  • Peer Reviewed