Elucidation of molecular basis of CCN family action as masterminds and its medical application
Project/Area Number |
24390415
|
Research Category |
Grant-in-Aid for Scientific Research (B)
|
Allocation Type | Partial Multi-year Fund |
Section | 一般 |
Research Field |
Functional basic dentistry
|
Research Institution | Okayama University |
Principal Investigator |
|
Co-Investigator(Kenkyū-buntansha) |
KUBOTA Satoshi 岡山大学, 大学院医歯薬学総合研究科, 教授 (90221936)
HATTORI Takako 岡山大学, 大学院医歯薬学総合研究科, 助教 (00228488)
NISHIDA Takashi 岡山大学, 大学院医歯薬学総合研究科, 准教授 (30322233)
AOYAMA Eriko 岡山大学, 大学院医歯薬学総合研究科, 助教 (10432650)
|
Project Period (FY) |
2012-04-01 – 2015-03-31
|
Project Status |
Completed (Fiscal Year 2014)
|
Budget Amount *help |
¥18,200,000 (Direct Cost: ¥14,000,000、Indirect Cost: ¥4,200,000)
Fiscal Year 2014: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2013: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000)
Fiscal Year 2012: ¥7,280,000 (Direct Cost: ¥5,600,000、Indirect Cost: ¥1,680,000)
|
Keywords | CCN2/CTGF / CCN ファミリー / CCN3/Nov / マスターマインド / 軟骨 / 骨 / 再生 / 変形性関節症 / CCNファミリー / 組織再生 / トランスジェニックマウス / CCN2 / CCN3 / FGFR2 / OPG / 半月板 / 骨芽細胞 |
Outline of Final Research Achievements |
We elucidated molecular mechanism of actions of CCN family proteins as masterminds by investigating physical interactions between CCN proteins and various molecules such as growth factors and their receptors, and by determining their final biological outcome in various cultured cells. We also generated transgenic mice overexpressing CCN2 in cartilage and found harmonized promotion of endochondral ossification in the TG mice, which would be a proof of function of a mastermind. Moreover, TSP-1 module among 4 independent modules of CCN2 had more potent action than that of full length CCN2 in cartilage regeneration in experimental osteoarthritis animal models, suggesting possible medical application of a CCN2 fragment for regenerative medicine for skeletal tissues. Furthermore, low intensity pulsed ultrasound induced gene expression of CCN2, aggrecan and collagen type II in chondrocytes, suggesting possible non-invasive application of CCN2 for cartilage regeneration in osteoarthritis.
|
Report
(4 results)
Research Products
(140 results)
-
-
-
-
-
-
-
[Journal Article] CCN2 as a novel molecule supporting energy metabolism of chondrocytes.2014
Author(s)
Maeda-Uematsu A, Kubota S, Kawaki H, Kawata K, Miyake Y, Hattori T, Nishida T, Moritani N, Lyons KM, Iida S, Takigawa M.
-
Journal Title
J Cell Biochem
Volume: 115
Issue: 5
Pages: 854-865
DOI
NAID
Related Report
Peer Reviewed / Open Access
-
-
-
-
-
-
-
-
-
-
[Journal Article] Regulation of CCN1 via the 3’-untranslated region.2013
Author(s)
Nakagawa, Y., Minato, M., Sumiyoshi, K., Maeda, A., Hara, C., Murase, Y., Nishida, T., Kubota, S. and Takigawa, M.:
-
Journal Title
J. Cell Commun. Signal., 7, 207-217, 2013.
Volume: 7
Issue: 3
Pages: 207-217
DOI
Related Report
Peer Reviewed
-
[Journal Article] CCN family member 2/connective tissue growth factor (CCN2/CTGF) has anti-aging effects that protect articular cartilage from age-related degenerative changes2013
Author(s)
Itoh, S., Hattori, T., Tomita, N., Aoyama, E., Yutani,Y., Yamashiro, T., Takigawa, M.
-
Journal Title
PLoS One
Volume: 8
Issue: 8
Pages: e71156-e71156
DOI
NAID
Related Report
Peer Reviewed
-
-
[Journal Article] miRNA-720 controls stem cell phenotype, proliferation and differentiation of human dental pulp cells2013
Author(s)
Hara ES, Ono M, Eguchi T, Kubota S, Pham HT, Sonoyama W, Tajima S, Takigawa M, Calderwood SK, Kuboki T.
-
Journal Title
PLoS One
Volume: 8(12)
Issue: 12
Pages: 1-11
DOI
NAID
Related Report
Peer Reviewed
-
-
[Journal Article] Novel chondrogenic and chondroprotective effects of the natural compound harmine.2013
Author(s)
Hara, E. S., Ono, M., Kubota, S., Sonoyama, W., Oida, Y., Hattori, T., Nishida, T., Furumatsu, T., Ozaki, T., Takigawa, M. and Kuboki, T
-
Journal Title
Biochimie
Volume: 95
Issue: 2
Pages: 347-381
DOI
Related Report
Peer Reviewed
-
[Journal Article] Anti-fibrotic effect of CCN3 accompanied by altered gene expression profile of the CCN family.2013
Author(s)
Abd El Kader, T., Kubota, S., Janune, D., Nishida, T., Hattori, T., Aoyama, E., Perbal, B., Kuboki, T. and Takigawa, M.
-
Journal Title
J. Cell Commun. Signal.
Volume: 7
Issue: 1
Pages: 11-18
DOI
Related Report
Peer Reviewed
-
-
[Journal Article] Cartilage–specific overexpression of CCN family member 2/connective tissue growth factor (CCN2/CTGF) stimulates insulin-like growth factor expression and bone growth2013
Author(s)
Tomita, N., Hattori, T., Ito, S., Aoyama, E., Yao, M., Yamashiro, T., Takigawa M.
-
Journal Title
PLoS One
Volume: 8
Issue: 3
Pages: e59226-e59226
DOI
NAID
Related Report
Peer Reviewed
-
-
-
[Journal Article] Role of low-density lipoprotein receptor related protein 1 (LRP1) in CCN2/connective tissue growth factor (CTGF) protein transport in chondrocytes.2012
Author(s)
Kawata., K., Kubota, S., Eguchi, T., Aoyama, E., Moritani, N., Kondo, S., Nishida, T., Takigawa, M.
-
Journal Title
J Cell Sci
Volume: 15
Pages: 2965-2972
DOI
Related Report
Peer Reviewed
-
-
-
-
-
-
-
-
[Presentation] Assessment of the regenerative effects of CCN2 independent modules on chondrocytes in vitro and osteoarturiritis models in vivo2014
Author(s)
Abd El Kdaer, T., Kubota, S., Nishida, T., Hattori, T., Aoyama, E., Janune, D., Hara, E. S., Ono, M., Tabata, Y., Kuboki, T., Takigawa, M.
Organizer
第37回日本分子生物学会
Place of Presentation
横浜
Year and Date
2014-11-25 – 2014-11-27
Related Report
-
-
-
-
-
-
-
-
-
-
-
-
-
[Presentation] The regenerative effect of CCN2 independent modules on chondrocytes in vitro and osteoarturiritis models in vivo2014
Author(s)
Abd El Kdaer, T., Kubota, S., Nishida, T., Hattori, T., Aoyama, E., Janune, D., Hara, E. S., Ono, M., Tabata, Y., Kuboki, T., Takigawa, M.
Organizer
第6回日本CCNファミリー研究会
Place of Presentation
岡山
Year and Date
2014-08-30
Related Report
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
[Presentation] Roles of CCN2 and CCN3 in skeletogenesis2013
Author(s)
Takigawa, M., Kubota, S., Hattori, T., Nishida, T., Apyama, E., Tomita, N., Itoh, S., Janune, D., Hoshijima, M.
Organizer
Seventh International Workshop on the CCN Family of Genes
Place of Presentation
Nice, France
Related Report
Invited
-
-
-
[Presentation] CCN4/Wisp-1 enhances cell migration during skin wound healing2013
Author(s)
Ono, M., Masaki, A., Sonoyama, W., Maeda, A., Hara, E. S., Yoshioka, Y., kubota, S., Takigawa, M., Young, M. F., Kuboki, T.
Organizer
Seventh International Workshop on the CCN Family of Genes
Place of Presentation
Nice, France
Related Report
-
[Presentation] Cartilage-specific overexpression of CCN3 modulates endochondral bone formation2013
Author(s)
Hattori, T., Ono, M., Hoshijima, M., Kadoya, K., Kuwahara, M., Miyake, Y., Kuboki, T., Takigawa, M.
Organizer
Seventh International Workshop on the CCN Family of Genes
Place of Presentation
Nice, France
Related Report
-
[Presentation] The novel role of CCN2 as a regeratory factor in RANK/RANKL/OPG system2013
Author(s)
Aoyama, E., Kubota, S., Nishida, T., Kawaki, H., Takigawa, M.
Organizer
Seventh International Workshop on the CCN Family of Genes
Place of Presentation
Nice, France
Related Report
-
-
[Presentation] CCN2 is up-regulated in cultured chondrocytes treated with low-intensity plused ultrasound (LIPUS)2013
Author(s)
Nishida, T., Kubota, S., Aoyama, E., Yamanaka, N., Takigawa, M.
Organizer
2nd joint meeting of IBMS-JSBMR
Place of Presentation
Kobe, Japan
Related Report
-
[Presentation] Crucial role of CCN2 in energy metabolism in chondrocytes2013
Author(s)
Maeda, A., Kubota, S., Miyake, Y., Kawata, K., Hattori, T., Nishida, T., Moritani, N., Kawaki, H., Lyons, K., Iida, S., Takigawa, M.
Organizer
2nd joint meeting of IBMS-JSBMR
Place of Presentation
Kobe, Japan
Related Report
-
[Presentation] Identification and characterization of chondrocytic fibroblast growth factor 1 as a molecular counterpart of CCN family member 22013
Author(s)
Abd El Kader, T., Kubota, S., Nishida, T., Hattori, T., Aoyama, E., Furumatsu, T., Kuboki, T., Takigawa, M.
Organizer
2nd joint meeting of IBMS-JSBMR
Place of Presentation
Kobe, Japan
Related Report
-
[Presentation] Cartilage-specific overexpression of CCN3 negatively regulates endochondral bone formation2013
Author(s)
Hattori, T., Ono, M., Hoshijima, M., Kadoya, K., Kuwahara, M., Oie, H., Miyake, Y., Kuboki, T., Takigawa, M.
Organizer
2nd joint meeting of IBMS-JSBMR
Place of Presentation
Kobe, Japan
Related Report
-
-
[Presentation] Essential role of CCN2 that supports the basal energy metabolism in chondrocytes2013
Author(s)
Maeda, A., Kubota, S., Miyake, Y., Kawata, K., Hattori, T., Nishida, T., Norifumi Moritani, N., Kawaki, H., Lyons, K. M., Iida, S., Takigawa, M.
Organizer
第86回日本生化学会大会
Place of Presentation
横浜
Related Report
-
-
-
-
-
-
-
-
-
[Presentation] Anti-fibrotic role of CCN3 through regulation of CCN family genes2013
Author(s)
Abd El Kdaer, T., Kubota, S., Janune, D., Nishida, T., Hattori, T., Aoyama, E., Perbal, B., Takigawa, M.
Organizer
The third international symposium of medical-dental-pharmaceutical education and research in Okayama
Place of Presentation
岡山
Related Report
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-