| Project/Area Number |
24390415
|
|---|
| Research Category |
Grant-in-Aid for Scientific Research (B)
|
| Allocation Type | Partial Multi-year Fund |
|---|
| Section | 一般 |
|---|
| Research Field |
Functional basic dentistry
|
|---|
| Research Institution | Okayama University |
|---|
Principal Investigator |
|
|---|
| Co-Investigator(Kenkyū-buntansha) |
KUBOTA Satoshi 岡山大学, 大学院医歯薬学総合研究科, 教授 (90221936)
HATTORI Takako 岡山大学, 大学院医歯薬学総合研究科, 助教 (00228488)
NISHIDA Takashi 岡山大学, 大学院医歯薬学総合研究科, 准教授 (30322233)
AOYAMA Eriko 岡山大学, 大学院医歯薬学総合研究科, 助教 (10432650)
|
|---|
| Project Period (FY) |
2012-04-01 – 2015-03-31
|
| Project Status |
Completed (Fiscal Year 2014)
|
| Budget Amount *help |
¥18,200,000 (Direct Cost: ¥14,000,000、Indirect Cost: ¥4,200,000)
Fiscal Year 2014: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2013: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000)
Fiscal Year 2012: ¥7,280,000 (Direct Cost: ¥5,600,000、Indirect Cost: ¥1,680,000)
|
|---|
| Keywords | CCN2/CTGF / CCN ファミリー / CCN3/Nov / マスターマインド / 軟骨 / 骨 / 再生 / 変形性関節症 / CCNファミリー / 組織再生 / トランスジェニックマウス / CCN2 / CCN3 / FGFR2 / OPG / 半月板 / 骨芽細胞 |
|---|
| Outline of Final Research Achievements |
We elucidated molecular mechanism of actions of CCN family proteins as masterminds by investigating physical interactions between CCN proteins and various molecules such as growth factors and their receptors, and by determining their final biological outcome in various cultured cells. We also generated transgenic mice overexpressing CCN2 in cartilage and found harmonized promotion of endochondral ossification in the TG mice, which would be a proof of function of a mastermind. Moreover, TSP-1 module among 4 independent modules of CCN2 had more potent action than that of full length CCN2 in cartilage regeneration in experimental osteoarthritis animal models, suggesting possible medical application of a CCN2 fragment for regenerative medicine for skeletal tissues. Furthermore, low intensity pulsed ultrasound induced gene expression of CCN2, aggrecan and collagen type II in chondrocytes, suggesting possible non-invasive application of CCN2 for cartilage regeneration in osteoarthritis.
|
