| Budget Amount *help |
¥17,810,000 (Direct Cost: ¥13,700,000、Indirect Cost: ¥4,110,000)
Fiscal Year 2015: ¥2,990,000 (Direct Cost: ¥2,300,000、Indirect Cost: ¥690,000)
Fiscal Year 2014: ¥4,550,000 (Direct Cost: ¥3,500,000、Indirect Cost: ¥1,050,000)
Fiscal Year 2013: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2012: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
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| Outline of Final Research Achievements |
Among angiogenic facotrs produced by tumor cells, vascular endothelial growth factor (VEGF) seems to be the most potent and pathophysiologically important, and its synthesis has been shown to be modulated through the activation of some transcription factors including HIF-1 function under hypoxic condition. We transferred HIF-1 decoy ODNs, composed of synthetic double-stranded DNA including consensus sequence of binding site of the HIF-1, into cultured cancer cells (SAS cells) by the HVJ-liposome method. The overexpression of VEGF by cancer cells stimulated by hypoxia could be apparently suppressed by the transfection of HIF-1 decoy ODNs, but not by mt-HIF-1 decoy ODNs. These results suggested that the HIF-1 decoy strategy would be effective for regulating tumor growth by reducing angiogenic acitivity of cancer cells through HIF-1-mediated gene transactivations.
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