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Development of experimental pathologic refractory periapical periodontitis model and investigation of its chemical supplemental methods

Research Project

Project/Area Number 24390424
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypePartial Multi-year Fund
Section一般
Research Field Conservative dentistry
Research InstitutionOsaka University

Principal Investigator

NOIRI Yuichiro  大阪大学, 歯学研究科(研究院), 准教授 (50218286)

Co-Investigator(Kenkyū-buntansha) EBISU Shigeyuki  大阪大学, 大学院歯学研究科, 理事副学長 (50116000)
AZAKAMI Hiroyuki  山口大学, 農学部, 准教授 (40263850)
MAEZONO Hazuki  大阪大学, 大学院歯学研究科, 医員 (00613390)
ASAHI Youko  大阪大学, 学院歯学研究科, 助教 (50456943)
Co-Investigator(Renkei-kenkyūsha) NAKANO Takayoshi  大阪大学, 大学院工学系研究科, 教授 (30243182)
Project Period (FY) 2012-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥18,200,000 (Direct Cost: ¥14,000,000、Indirect Cost: ¥4,200,000)
Fiscal Year 2014: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2013: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2012: ¥7,930,000 (Direct Cost: ¥6,100,000、Indirect Cost: ¥1,830,000)
Keywordsバイオフィルム / 難治性根尖性歯周炎 / 根尖孔外 / マイクロCT / 抗バイオフィルム薬 / ラット / 高病原化細菌 / Eikenella corrodens / マイクロCT / マイクロCT / EIkenella corrodens / 高病原化細 / マイクロCT
Outline of Final Research Achievements

The purpose of this research challenge is to search on the model to develop a relationship of pathology and extraradicular biofilm of refractory periapical periodontitis and to evaluate the usefulness of chemical control method against this intractable diseases. We revealed for the first time that the presence of extraradicular biofilm is involved in the increase of apical lesions by using the developed model in vivo. We began the development of the root canal treatment (RCT) model instead of the development of the single bacterial species extraradicular biofilm model, because of the difficulty of infection by single bacterial species, and the RCT model almost succeeded. Thus, we will publush the results in the future. On the other hand, in search of highly pathogenic bacterium associated with biofilms, we reported that the pathogenicity of Eikenella corrodens become highly universally and LuxS affects biofilm maturation and detachment.

Report

(4 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Annual Research Report
  • 2012 Annual Research Report
  • Research Products

    (38 results)

All 2015 2014 2013 2012

All Journal Article (13 results) (of which Peer Reviewed: 10 results,  Open Access: 5 results,  Acknowledgement Compliant: 3 results) Presentation (23 results) (of which Invited: 2 results) Book (2 results)

  • [Journal Article] Inhibition of polysaccharide synthesis by the sinR orthologue PGN_0088 is indirectly associated with the penetration of Porphyromonas gingivalis biofilms by macroride antibiotics2015

    • Author(s)
      Yamamoto R. Noiri Y. Yamaguchi M. Asahi Y. Maezono H. Ebisu S. Hayashi M.
    • Journal Title

      Microbiology

      Volume: 161 Pages: 422-429

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Simple observation of Streptococcus mutans biofilm by scanning electron microscopy using ionic liquids2015

    • Author(s)
      Asahi Y. Miura J. Tsyda T. Kawabata S. Tsunashima K. Noiri Y. Sakata T. Ebisu S. Hayashi M.
    • Journal Title

      AMB express

      Volume: 5 Issue: 1 Pages: 6-14

    • DOI

      10.1186/s13568-015-0097-4

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Promotion of endodontic lesions in rats by a novel extraradicular model using obturation materials2014

    • Author(s)
      Kuremoto K. Noiri Y. Ishimoto T. Yoneda N. Yamamoto R. Maezono H. Nakano T. Hayashi M. Ebisu S
    • Journal Title

      Applied and Environmental Microbiology

      Volume: 80 Issue: 13 Pages: 3804-3810

    • DOI

      10.1128/aem.00421-14

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] 新時代の歯内療法:エビデンスとアートの融合 2.難治症例の実態と対応 バイオフィルム感染症にフォーカスして2014

    • Author(s)
      朝日陽子、野杁由一郎
    • Journal Title

      歯科医療

      Volume: 夏号 Pages: 12-19

    • Related Report
      2014 Annual Research Report
  • [Journal Article] 巻頭SCIENCE 1枚の写真から 第7回歯内療法の夜明け2014

    • Author(s)
      野杁由一郎
    • Journal Title

      ザ・クインテッセンス

      Volume: 33 Pages: 3-5

    • Related Report
      2014 Annual Research Report
  • [Journal Article] 歯根の肥大および湾曲を伴う上顎大臼歯の自家移植症例2014

    • Author(s)
      呉本勝隆 前薗葉月 北川蘭奈 竹田かほる 新野侑子 松下健太 伊藤祥作 野杁由一郎 林 美加子
    • Journal Title

      日本歯科保存学会誌

      Volume: 57 Pages: 589-596

    • NAID

      130005005960

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Unstable Mutant Lysozymes are Degraded through the Interaction with Calnexin Homologue Cne1p in Saccharomyces cerevisiae2014

    • Author(s)
      Azakami H. Uehara M. Matsuo R. Yamashita Y. Usui M. Kato A.
    • Journal Title

      Biosci. Biotechol. Biochem

      Volume: 78 Issue: 7 Pages: 1263-1269

    • DOI

      10.1080/09168451.2014.918486

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Effects of the tea catechin epigallocatechin gallate on Porphyromonas gingivalis biofilms2014

    • Author(s)
      Asahi Y. et al.
    • Journal Title

      Jouranl of Applied Microbiology

      Volume: 116 Issue: 5 Pages: 1164-1171

    • DOI

      10.1111/jam.12458

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] LuxS Affects Biofilm Maturation and Detachment of the Periodontopathogenic Bacterium Eikenella corrodens2013

    • Author(s)
      MM. Karim, T. Hisamoto, T. Matsunaga, Y. Asahi, Y. Noiri, S. Ebisu, A. Kato, H. Azakami
    • Journal Title

      Journal of Bioscience and Bioengineering

      Volume: 116 Issue: 3 Pages: 313-318

    • DOI

      10.1016/j.jbiosc.2013.03.013

    • NAID

      110009657226

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The Periodontopathogenic Bacterium <i>Eikenella corrodens</i> Produces an Autoinducer-2-Inactivating Enzyme2013

    • Author(s)
      MM. Karim, A. Nagao, FJ. Mansur, T. Matsunaga, Y. Akakabe, Y. Noiri, S. Ebisu, A. Kato, H. Azakami
    • Journal Title

      Bioscience, Biotechnology, and Biochemistry

      Volume: 77 Issue: 5 Pages: 1080-1085

    • DOI

      10.1271/bbb.130047

    • NAID

      10031177600

    • ISSN
      0916-8451, 1347-6947
    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] The sinR ortholog PGN_0088 encodes a transcriptional regulator that inhibits polysaccharide synthesis in Porphyromonas gingivalis ATCC 33277 biofilms2013

    • Author(s)
      Yamamoto R, Noiri Y, Yamaguchi M, Asahi Y, Maezono H, Kuboniwa M, Hayashi M, Ebisu S
    • Journal Title

      PLoS One

      Volume: Vol.8, No.2 Issue: 2 Pages: e56017-e56017

    • DOI

      10.1371/journal.pone.0056017

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] う蝕象牙質除去後の残存細菌にEr : YAGレーザーが与える影響2013

    • Author(s)
      高橋雄介, ら
    • Journal Title

      日歯保存誌

      Volume: 56 Pages: 1-8

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 根尖性歯周炎と細菌-バイオフィルム形成を中心に-2013

    • Author(s)
      山口幹代, 野杁由一郎
    • Journal Title

      歯科医療

      Volume: 27 Pages: 27-34

    • Related Report
      2012 Annual Research Report
  • [Presentation] 実験病理学的根尖孔外バイオフィルムモデルの開発2015

    • Author(s)
      松井沙織 野杁由一郎 呉本勝隆 米田直道 恵比須繁之 林美加子
    • Organizer
      第12回日本顕微鏡歯科学会学術大会
    • Place of Presentation
      東京 一橋講堂
    • Year and Date
      2015-04-18 – 2015-04-19
    • Related Report
      2014 Annual Research Report
  • [Presentation] Autoinducer-2 inactivation by outer membrane porin in periodontopathogenic bacterium Eikenella corrodens2015

    • Author(s)
      Fariha Jasin Mansur 森重なつみ 野杁由一郎 阿座上弘行
    • Organizer
      日本農芸化学会
    • Place of Presentation
      岡山
    • Year and Date
      2015-03-26 – 2015-03-29
    • Related Report
      2014 Annual Research Report
  • [Presentation] 歯周病原性細菌Eikenella corrodensにおけるゲノム再編による溶血活性の発現2015

    • Author(s)
      高原沙里 下川床愛 野杁由一郎 阿座上弘行
    • Organizer
      日本農芸化学会
    • Place of Presentation
      岡山
    • Year and Date
      2015-03-26 – 2015-03-29
    • Related Report
      2014 Annual Research Report
  • [Presentation] Quantitative Analysis of Human Dental Biofilms by New in situ Model2015

    • Author(s)
      Wake N. Asahi Y. Noiri Y. Machi H. Ebisu S. Hayashi M.
    • Organizer
      92nd IADR
    • Place of Presentation
      Boston USA
    • Year and Date
      2015-03-11 – 2015-03-14
    • Related Report
      2014 Annual Research Report
  • [Presentation] レーザーによりデンタルバイオフィルムを制御して新規治療法・予防法の開発・臨床応用へ2015

    • Author(s)
      野杁由一郎 朝日陽子 高橋雄介 恵比須繁之 林美加子
    • Organizer
      大阪大学未来戦略機構国際シンポジウムOpto Osaka 2015
    • Place of Presentation
      大阪 ナレッジキャピタル
    • Year and Date
      2015-01-14
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] Quantitative Analysis of Human Dental Biofilms by New in situ Model2014

    • Author(s)
      Wake N. Asahi Y. Noiri Y. Machi H. Ebisu S. Hayashi M.
    • Organizer
      62nd Annual Meeting of the Japanese Association for Dental Research
    • Place of Presentation
      大阪 KKR大阪
    • Year and Date
      2014-12-04 – 2014-12-05
    • Related Report
      2014 Annual Research Report
  • [Presentation] ラット根尖孔外バイオフィルムモデルの改良2014

    • Author(s)
      呉本勝隆 野杁由一郎 米田直道 松井沙織 石本卓也 中野貴由 恵比須繁之 林美加子
    • Organizer
      第141回日本歯科保存学会秋季学術大会
    • Place of Presentation
      山県 山形テルサ
    • Year and Date
      2014-10-30 – 2014-10-31
    • Related Report
      2014 Annual Research Report
  • [Presentation] ラット感染根管治療モデルの開発2014

    • Author(s)
      米田直道 野杁由一郎 呉本勝隆 松井沙織 石本卓也 中野貴由 恵比須繁之 林美加子
    • Organizer
      第141回日本歯科保存学会秋季学術大会
    • Place of Presentation
      山県 山形テルサ
    • Year and Date
      2014-10-30 – 2014-10-31
    • Related Report
      2014 Annual Research Report
  • [Presentation] デンタルバイオフィルムの形成と制御に関する包括的 in situ 解析~各細菌属の経時的定量解析~2014

    • Author(s)
      和氣菜々子 朝日陽子 町博之 野杁由一郎 林美加子 恵比須繁之
    • Organizer
      第141回日本歯科保存学会秋季学術大会
    • Place of Presentation
      山県 山形テルサ
    • Year and Date
      2014-10-30 – 2014-10-31
    • Related Report
      2014 Annual Research Report
  • [Presentation] Streptococcus sanguinisが産生する過酸化水素は好中球の細胞死を誘導する2014

    • Author(s)
      住岡龍一 中田匡宣 縄端重忠 林美加子
    • Organizer
      第141回日本歯科保存学会秋季学術大会
    • Place of Presentation
      山県 山形テルサ
    • Year and Date
      2014-10-30 – 2014-10-31
    • Related Report
      2014 Annual Research Report
  • [Presentation] 歯周病原性細菌Eikenella corrodensのX-プロリルアミノペプチダーゼが溶血に関与する2014

    • Author(s)
      高原沙里 下川床愛 野杁由一郎 阿座上弘行
    • Organizer
      日本生物工学会
    • Place of Presentation
      札幌
    • Year and Date
      2014-09-09 – 2014-09-11
    • Related Report
      2014 Annual Research Report
  • [Presentation] 歯周病原性細菌Eikenella corrodensの外膜ポーリンによるオートインデューサー2の不活化機構2014

    • Author(s)
      森なつみら Fariha Jasin Mansur 飯田亮平 野杁由一郎 阿座上弘行
    • Organizer
      日本生物工学会
    • Place of Presentation
      札幌
    • Year and Date
      2014-09-09 – 2014-09-11
    • Related Report
      2014 Annual Research Report
  • [Presentation] MTAを用いて歯根尖切除術と同時に穿孔部封鎖術を施した症例2014

    • Author(s)
      北川蘭奈 北川晴朗 野杁由一郎 林美加子
    • Organizer
      日本歯内療法学会西日本支部会 第14回研修会
    • Place of Presentation
      大阪歯科大学
    • Year and Date
      2014-09-06 – 2014-09-07
    • Related Report
      2014 Annual Research Report
  • [Presentation] Purification and characterization of autoinducer-2 inactivating enzyme in periodontopathogenic bacterium Eikenella corrodens2014

    • Author(s)
      Fariha M. Karim M. Noiri Y. Azakami H.
    • Organizer
      IUMS
    • Place of Presentation
      Canada, Montereal
    • Year and Date
      2014-07-27 – 2014-08-01
    • Related Report
      2014 Annual Research Report
  • [Presentation] 難治症例の実態と対応:バイオフィルム感染症にフォーカスして2014

    • Author(s)
      野杁由一郎
    • Organizer
      第35回日本歯内療法学会学術大会
    • Place of Presentation
      新潟 朱鷺メッセ
    • Year and Date
      2014-07-12 – 2014-07-13
    • Related Report
      2014 Annual Research Report
    • Invited
  • [Presentation] Time course micro-CT analysis of periapical lesions using extraradicular biofilm model in rat.2013

    • Author(s)
      Kuremoto K et al.
    • Organizer
      9th International Federation of Endodontic Association
    • Place of Presentation
      Tokyo, Tokyo International Forum
    • Related Report
      2013 Annual Research Report
  • [Presentation] Investigation on factors causing refractory periapical periodontitis in general practices2013

    • Author(s)
      Yamaguchi M et al.
    • Organizer
      9th International Federation of Endodontic Association
    • Place of Presentation
      Tokyo, Tokyo International Forum
    • Related Report
      2013 Annual Research Report
  • [Presentation] デンタルバイオフィルム形成に関するin situ解析~微細形態学的観察~2013

    • Author(s)
      朝日陽子 ら
    • Organizer
      第139回日本歯科保存学会秋季学術大会
    • Place of Presentation
      秋田アトリオン
    • Related Report
      2013 Annual Research Report
  • [Presentation] デンタルバイオフィルム形成と制御に関する包括的in situ解析~定量的3次元検索~2013

    • Author(s)
      栗木菜々子 ら
    • Organizer
      第139回日本歯科保存学会秋季学術大会
    • Place of Presentation
      秋田アトリオン
    • Related Report
      2013 Annual Research Report
  • [Presentation] 外科的歯内療法におけるCBCTおよびMTAの有用性:症例報告2013

    • Author(s)
      古谷 優  ら
    • Organizer
      日本歯内療法学会西日本支部会第13回研修会
    • Place of Presentation
      大阪歯科大学
    • Related Report
      2013 Annual Research Report
  • [Presentation] 根尖孔外バイオフィルムモデルにおけるラット根尖病巣のマイクロCT解析2012

    • Author(s)
      呉本勝隆, ら
    • Organizer
      日本歯科保存学会2012年度秋季学術大会(第137回)
    • Place of Presentation
      広島国際会議場
    • Year and Date
      2012-11-23
    • Related Report
      2012 Annual Research Report
  • [Presentation] Role of PGN 0088 of Porphyromonas gingivakis -Biofilm formation and antibiofilm effect of macrolide antibiotics-2012

    • Author(s)
      Yamamoto R, et al.
    • Organizer
      6_<th> ASM Conference on Biofilms
    • Place of Presentation
      Miami, Florida, USA
    • Year and Date
      2012-10-03
    • Related Report
      2012 Annual Research Report
  • [Presentation] Dispersal of Porphyromonas gingivakis biofilm induced by environmental factors and proteases2012

    • Author(s)
      Yamaguchi M, et al.
    • Organizer
      6_<th> ASM Conference on Biofilms
    • Place of Presentation
      Miami, Florida, USA
    • Year and Date
      2012-10-03
    • Related Report
      2012 Annual Research Report
  • [Book] エンドドンティックス21 改訂4版2014

    • Author(s)
      野杁由一郎ら
    • Total Pages
      269
    • Publisher
      永末出版
    • Related Report
      2014 Annual Research Report
  • [Book] '14 Quntessence 6月号 (分担執筆)2014

    • Author(s)
      野杁由一郎
    • Total Pages
      233
    • Publisher
      クインテッセンス出版
    • Related Report
      2013 Annual Research Report

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Published: 2012-04-24   Modified: 2019-07-29  

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