Clinical application of a novel disinfection system based on local administration of low concentration hydroxyl radicals

• Project/Area Number: 24390430
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Prosthetic dentistry
• Research Institution: Tohoku University
• Principal Investigator: SASAKI Keiichi 東北大学, 歯学研究科(研究院), 教授 (30178644)
• Co-Investigator(Kenkyū-buntansha): KANNO Taro 東北大学, 大学院歯学研究科, 助教 (30302160) ; HAYASHI Eisei 東北大学, 大学院歯学研究科, 大学院非常勤講師 (60375102) ; IKAI Hiroyo 東北大学, 大学院歯学研究科, 助教 (20431588) ; NAKAMURA Keisuke 東北大学, 大学院歯学研究科, 助教 (30431589) ; NIWANO Yoshimi 東北大学, 大学院歯学研究科, 教授 (40375184) ; KOHNO Masahiro 東京工業大学, 大学院生命理工学研究科, 教授 (70333226)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥17,030,000 (Direct Cost: ¥13,100,000、Indirect Cost: ¥3,930,000); Fiscal Year 2014: ¥6,110,000 (Direct Cost: ¥4,700,000、Indirect Cost: ¥1,410,000); Fiscal Year 2013: ¥5,590,000 (Direct Cost: ¥4,300,000、Indirect Cost: ¥1,290,000); Fiscal Year 2012: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
• Keywords: ヒドロキシルラジカル / 活性酸素 / フリーラジカル / 過酸化水素光分解 / 歯周病治療器 / ラジカル殺菌 / 過酸化水素

Outline of Final Research Achievements

Bactericidal effect and biological safety of a novel therapeutic device utilizing a disinfection technique based on photolysis of hydrogen peroxide for the treatment of periodontal disease were evaluated in the present study. In addition, clinical study was conducted to compare the efficacy of the device in the treatment of periodontitis with that of conventional root debridement. In vitro bactericidal tests demonstrated that device exerted high bactericidal effect, whereas in vivo test showed that it did not cause adverse effect on the oral mucosa of rats. In the clinical study, it was demonstrated that the treatment using the device significantly reduced the probing pocket depth 3 months after the treatment than conventional root debridement. Thus, it is suggested that the periodontal treatment using the device may improve the effect of non-surgical periodontal therapy.

Research Products

• [Journal Article] Synergistic interaction between wavelength of light and concentration of H2O2 in bactericidal activity of photolysis of H2O2. (2015)
  • Author(s): Toki, T., Nakamura, K., Kurauchi, M., Kanno, T., Katsuda, Y., Ikai, H., Hayashi, E., Egusa, H., Sasaki, K., Niwano, Y.
  • Journal Title: Journal of Bioscience and Bioengineering Volume: 19(3) Issue: 3 Pages: 1358-1362
  • DOI: 10.1016/j.jbiosc.2014.08.015
  • NAID: 210000004988
  • Peer Reviewed
• [Journal Article] Cytoprotective effect of short-term pretreatment with proanthocyanidin on human gingival fibroblasts exposed to harsh environmental conditions. (2014)
  • Author(s): Kurauchi M, Niwano Y, Shirato M, Kanno T, Nakamura K, Egusa H, Sasaki K.
  • Journal Title: PLoS One Volume: 9 Issue: 11 Pages: e113403-e113403
  • DOI: 10.1371/journal.pone.0113403
  • Peer Reviewed
• [Journal Article] Acceleration of proliferative response of mouse fibroblasts by short-time pretreatment with polyphenols. (2014)
  • Author(s): Tsuruya, M., Niwano, Y., Nakamura, K., Kanno, T., Nakashima, T., Egusa, H., Sasaki, K.
  • Journal Title: Applied Biochemistry and Biotechnology. Volume: 174(6) Issue: 6 Pages: 2223-2235
  • DOI: 10.1007/s12010-014-1124-7
  • Peer Reviewed
• [Journal Article] Postantibiotic effect of disinfection treatment by photolysis of hydrogen peroxide. (2014)
  • Author(s): Odashima, Y., Nakamura, K., Ikai, H., Kanno, T., Meirelles, L., Sasaki, K., Niwano, Y.
  • Journal Title: Journal of Chemotherapy Volume: 26(2): Issue: 2 Pages: 92-100
  • DOI: 10.1179/1973947813y.0000000114
  • Peer Reviewed
• [Journal Article] Influence of a new denture cleaning technique based on photolysis of H₂O₂ the mechanical properties and color change of acrylic denture base resin (2013)
  • Author(s): Nakahara T, Harada A, Yamada Y, Odashima Y, Nakamura K, Inagaki R, Kanno T, Sasaki K, Niwano Y
  • Journal Title: Dental Materials Journal Volume: 32 Issue: 4 Pages: 529-536
  • DOI: 10.4012/dmj.2013-047
  • NAID: 10031178633
  • ISSN: 0287-4547, 1881-1361
  • Peer Reviewed
• [Journal Article] In vitro evaluation of the risk of inducing bacterial resistance to disinfection treatment with photolysis of hydrogen peroxide (2013)
  • Author(s): Ikai, H., Odashima, Y., Kanno, T., Nakamura, K., Shirato, M., Sasaki, K., Niwano, Y
  • Journal Title: PLoS ONE Volume: 8(11) Issue: 11 Pages: e81316-e81316
  • DOI: 10.1371/journal.pone.0081316
  • Peer Reviewed
• [Journal Article] Microbial resistance in relation to catalase activity to oxidative stress induced by photolysis of H202 (2012)
  • Author(s): Nakamura K, Kanno T, Mokudai T, Iwasawa A, Niwano y Kohno M
  • Journal Title: Microbiol Immunol Volume: 56 Issue: 1 Pages: 48-55
  • DOI: 10.1111/j.1348-0421.2011.00400.x
  • Peer Reviewed
• [Journal Article] Synergistic Effect of Thermal Energy on Bactericidal Action of Photolysis of H_2O_2 in Relation to Acceleration of Hydroxyl Radical Generation (2012)
  • Author(s): Shirato M, Ikai H, Nakamura K, Hayashi E, Kanno T, Sasaki K, Kohno M, Niwano Y
  • Journal Title: Antimicrob Agents Chemother Volume: 56 Issue: 1 Pages: 295-301
  • DOI: 10.1128/aac.05158-11
  • Peer Reviewed
• [Journal Article] Topical treatment of oral cavity wounded skin with a new disinfection system utilizing photolysis of hydrogen peroxide in rats (2012)
  • Author(s): Yamada Y; Mokudai T, Nakamura K, Hayashi E, Kawana y Kanno T, Sasaki K, Niwano Y
  • Journal Title: J Toxicol Sci Volume: 37 Pages: 329-335
  • NAID: 10030126757
  • Peer Reviewed
• [Journal Article] Determination of reactive oxygen species generated by phorbol 12-myristate 13-acetate.stimulated oral polymorphonuclear cells from healthy human volunteers without any dental problems (2012)
  • Author(s): Yaekashiwa N, Sato E, Nakamura K, Iwasawa A, Kudo, A, Kanno T, Kohno M, Niwano Y
  • Journal Title: Arch Oral Biol Volume: 57 Issue: 6 Pages: 636-641
  • DOI: 10.1016/j.archoralbio.2011.10.020
  • Peer Reviewed
• [Journal Article] Bactericidal action of photogenerated singlet oxygen from photosensitizers used in plaque disclosing agents (2012)
  • Author(s): Ishiyama K, Nakamura K, Ikai H, Kanno T, Kohno M, Sasaki K, Niwano Y
  • Journal Title: PLoS ONE Volume: 7 Issue: 5 Pages: e37871-e37871
  • DOI: 10.1371/journal.pone.0037871
  • Peer Reviewed
• [Journal Article] Influence of grain size and veneer firing process on flexural strength of zirconia ceramics (2012)
  • Author(s): Nakamura K, Adlfsson E,Milleding P, Kanno T, Ortengren U
  • Journal Title: Eur J Oral Sci Volume: 120 Issue: 3 Pages: 249-254
  • DOI: 10.1111/j.1600-0722.2012.00958.x
  • Peer Reviewed
• [Journal Article] In vitro and in vivo anti-Staphylococcus aureus activities of a new disinfection system utilizing photolysis of hydrogen peroxide (2012)
  • Author(s): Hayashi, E., Mokudai, T., Yamada, Y., Nakamura, K., Kanno, T., Sasaki, K., Niwano, Y
  • Journal Title: J. Biosci. Bioeng Volume: 114(2) Issue: 2 Pages: 193-197
  • DOI: 10.1016/j.jbiosc.2012.03.020
  • NAID: 110009495428
  • Peer Reviewed
• [Journal Article] Novel denture cleaning system based on hydroxyi radical disinfection. (2012)
  • Author(s): Kanno T, Nakamura K, Ikai H, Hayashi E, Shirato M, Mokudai T, Iwasawa A, Kohno M, Niwano Y, Sasaki K
  • Journal Title: Int J Prosthodont Volume: 25 Pages: 376-380
  • Peer Reviewed
• [Journal Article] Literature review of the role of hydroxyl radicals in chemically-induced mutagenicity and carcinogenicity for the risk assessment of a disinfection system utilizing photolysis of hydrogen peroxide (2012)
  • Author(s): Kanno T, Nakamura K, Ikai H, Kikuchi K, Sasaki K, Niwano Y
  • Journal Title: Journal of Clinical Biochemistry and Nutrition Volume: 51 Issue: 1 Pages: 9-14
  • DOI: 10.3164/jcbn.11-105
  • NAID: 130004879280
  • ISSN: 0912-0009, 1880-5086
  • Peer Reviewed
• [Journal Article] Corrosive effect of disinfection solution containing hydroxyl radicals generated by photolysis of H₂O₂ on dental metals (2012)
  • Author(s): Nakamura K, Yamada Y, Takada Y, Mokudai T, Ikai H, Inagaki R, Kanno T, Sasaki K, Kohno M, Niwano Y
  • Journal Title: Dental Materials Journal Volume: 31 Issue: 6 Pages: 941-946
  • DOI: 10.4012/dmj.2012-098
  • NAID: 10031126072
  • ISSN: 0287-4547, 1881-1361
  • Peer Reviewed
• [Journal Article] Presence of hydrogen peroxide a source of hydroxyi radicals in acid electrolyzed water (2012)
  • Author(s): Mokudai T, Nakamura K, Kann T, Niwano Y
  • Journal Title: PLoS ONE Volume: 7 Issue: 9 Pages: e46392-e46392
  • DOI: 10.1371/journal.pone.0046392
  • Peer Reviewed
• [Journal Article] Bactericidal action of photo-irradiated gallic acid via reactive oxygen species formation (2012)
  • Author(s): Nakamura K, Yamada Y, Ikai H, Kanno T, Sasaki K, Niwano Y
  • Journal Title: J Agric Food Chem Volume: 60 Pages: 1004810054-1004810054
  • Peer Reviewed
• [Presentation] 過酸化水素光分解殺菌技術における光の波長と過酸化水素濃度の相乗作用 (2014)
  • Author(s): 倉内美智子，唐木英俊，中村圭祐，菅野太郎，勝田悠介，猪飼紘代，林栄成，佐々木啓一，庭野吉己．
  • Organizer: 日本防菌防黴学会第41回年次大会
  • Place of Presentation: 東京
  • Year and Date: 2014-09-25 – 2014-09-26
• [Presentation] 過酸化水素の光分解および水の超音波分解を併用したヒドロキシルラジカル生成系の殺菌効果 (2014)
  • Author(s): 生宏、中村圭祐、菅野太郎、佐々木啓一、庭野吉己
  • Organizer: 日本防菌防黴学会台41回年次大会
  • Place of Presentation: 東京
  • Year and Date: 2014-09-25 – 2014-09-26
• [Presentation] Bactericidal action of photogenerated singlet oxygen from photosensitizers used in plaque disclosing agents (2013)
  • Author(s): Ishiyama, K., Nakamura, K., Ikai, H., Kanno, T., Sasaki, K., Niwano, Y
  • Organizer: Innovative Research for Biosis-Abiosis Intelligent Interface Summer Seminar 2013
  • Place of Presentation: 宮城県遠刈田郡
• [Presentation] Advantages of new disinfection treatment utilizing photolysis of hydrogen peroxide - Low risk of inducing bacterial resistance and postantibiotic effect (2013)
  • Author(s): Ikai, H., Odashima, Y., Nakamura, K., Shirato, M., Kanno, T., Sasaki, K., Niwano, Y
  • Organizer: Innovative Research for Biosis-Abiosis Intelligent Interface Summer Seminar 2013
  • Place of Presentation: 宮城県遠刈田郡
• [Presentation] 電気分解で生成された酸性電解水中に存在する水酸化ラジカルは殺菌活性に寄与するか？ (2013)
  • Author(s): 目代貴之、中村圭祐、菅野太郎、庭野吉己
  • Organizer: 第40回日本防菌防黴学会
  • Place of Presentation: 大阪
• [Presentation] 過酸化水素光分解殺菌法の短時間処理が残存細菌に及ぼす増殖抑制効果 (2013)
  • Author(s): 小田島優、中村圭祐、猪飼紘代、菅野太郎、佐々木啓一、庭野吉己
  • Organizer: 第40回日本防菌防黴学会
  • Place of Presentation: 大阪
• [Presentation] 過酸化水素光分解殺菌法の耐性誘導試験 (2013)
  • Author(s): 猪飼紘代 、中村圭祐、小田島優、白土翠、菅野太郎、庭野吉己
  • Organizer: 第40回日本防菌防黴学会
  • Place of Presentation: 大阪
• [Presentation] 各種ポリフェノールに対する青色可視光照射で得られる殺菌効果の比較検討 (2013)
  • Author(s): 中村圭祐、石山希里香、猪飼紘代、菅野太郎、庭野吉己
  • Organizer: 第40回日本防菌防黴学会
  • Place of Presentation: 大阪
• [Presentation] 歯科疾患のない健常人から採取したPMA刺激口腔内多形核白血球が産生する活性酸素種 (2012)
  • Author(s): 庭野吉己、八重柏典子、佐藤恵美子、中村圭祐、菅野太郎、岩沢篤郎、河野雅弘
  • Organizer: 第64回日本酸化ストレス学会学術集会
  • Place of Presentation: 徳島県郷土文化会館(徳島)
• [Presentation] 各種光感受性プラーク染色剤から生成する一重項酸素による殺菌効果の比較検討 (2012)
  • Author(s): 石山希里香,中村圭祐,猪飼紘代,菅野太郎,佐々木啓一,庭野吉己
  • Organizer: 第64回日本酸化ストレス学会学術集会
  • Place of Presentation: 徳島県郷土文化会館(徳島)
• [Presentation] 過酸化水素光分解を利用した新規歯科治療器のラット口腔粘膜および創傷皮膚への局所応用における急性の毒性学的評価 (2012)
  • Author(s): 山田康友、目代貴之、中村圭祐、林栄成、菅野太郎、佐々木啓一、庭野吉己
  • Organizer: 第64回日本酸化ストレス学会学術集会
  • Place of Presentation: 徳島県郷土文化会館(徳島)
• [Presentation] 過酸化水素光分解を応用した新しい殺菌システムの黄色ブドウ球菌に対するmvitroおよびin vivo抗菌活性 (2012)
  • Author(s): 林栄成、目代貴之、山田康友、中村圭祐、菅野太郎、佐々木啓一、庭野吉己
  • Organizer: 第64回日本酸化ストレス学会学術集会
  • Place of Presentation: 徳島県郷土文化会館(徳島)
• [Presentation] In vivo evaluation of a new disinfection system utilizing photolysis ofhydrogen peroxide (2012)
  • Author(s): Niwano Y, Hayashi E, Yamada Y, Mokudai T, Nakamura K, Ikai H, Kanno T, Sasaki K
  • Organizer: Innovative Research for Biosis-Abiosis Intelligent Interface Summer Seminar 2012
  • Place of Presentation: 蔵王ロイヤルホテル(宮城)
• [Presentation] Novel Denture Cleaning System Based on Hydroxyi Radical Disinfection (2012)
  • Author(s): Kanno T, Nakamura K, Ikai H, Hayashi E, Shirato M, Mokudai T,Iwasawa A,, Niwano Y, Sasaki K
  • Organizer: Innovative Research for Biosis-Abiosis Intelligent Interface Summer Seminar 2012
  • Place of Presentation: 蔵王ロイヤルホテル(宮城)
• [Presentation] 過酸化水素光分解を応用したラジカル殺菌技術のin VIVO殺菌活性および安全性評価 (2012)
  • Author(s): 庭野吉己、林栄成、山田康友、目代貴之、中村圭祐、菅野太郎、佐々木啓一
  • Organizer: 第39回日本防菌防黴学会年次大会
  • Place of Presentation: 品川区立総合区民会館(東京)
• [Presentation] 歯垢染色剤を用いた光線力学殺菌療法の比較検討 (2012)
  • Author(s): 石山希里香,中村圭祐,猪飼紘代,菅野太郎,佐々木啓一,庭野吉己
  • Organizer: 第39回日本防菌防黴学会年次大会
  • Place of Presentation: 品川区立総合区民会館(東京)
• [Presentation] ポリフェノールへの青色可視光照射により生成される活性酸素を応用した新規殺菌技術 (2012)
  • Author(s): 中村圭祐、山田康友、猪飼紘代、菅野太郎、佐々木啓一、庭野吉己
  • Organizer: 第39回日本防菌防黴学会年次大会
  • Place of Presentation: 品川区立総合区民会館(東京)
• [Presentation] 過酸化水素光分解殺菌へのカテキン類.生薬の併用効果 (2012)
  • Author(s): 猪飼紘代、白土翠、中村圭祐、菅野太郎、佐々木啓一、庭野吉己
  • Organizer: 第39回日本防菌防黴学会年次大会
  • Place of Presentation: 品川区立総合区民会館(東京)
• [Presentation] 市販オキシドールの光分解殺菌効果 (2012)
  • Author(s): 小山田晃、猪飼紘代、中村圭祐、菅野太郎、佐々木啓一、庭野吉己
  • Organizer: 第39回日本防菌防黴学会年次大会
  • Place of Presentation: 品川区立総合区民会館(東京)
• [Presentation] 過酸化水素光分解殺菌法に対する微生物の抵抗性とカタラーゼ活性の関連性 (2012)
  • Author(s): 中村圭祐、菅野太郎、目代貴之、林栄成、猪飼紘代、佐々木啓一、庭野吉己
  • Organizer: 第39回日本防菌防黴学会年次大会
  • Place of Presentation: 大阪国際会議場(大阪)
• [Presentation] 過酸化水素光分解殺菌法に対する熱エネルギーの相乗的効果 (2012)
  • Author(s): 白土翠、猪飼紘代、中村圭祐、林栄成、菅野太郎、佐々木啓一、庭野吉己
  • Organizer: 第39回日本防菌防黴学会年次大会
  • Place of Presentation: 大阪国際会議場(大阪)
• [Presentation] プラーク染色剤を用いた新しい光線力学殺菌療法の基礎的検討 (2012)
  • Author(s): 石山希里香、中村圭祐、猪飼紘代、菅野太郎、佐々木啓一、庭野吉己
  • Organizer: 第39回日本防菌防黴学会年次大会
  • Place of Presentation: 大阪国際会議場(大阪)
• [Book] 歯界展望 (2013)
  • Author(s): 菅野太郎、中村圭祐
  • Total Pages: 274
  • Publisher: 医歯薬出版