Developments of scaffolds and processing technology to maximize the osteogenic potential of cultured periosteal sheets
| Project/Area Number |
24390443
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Dental engineering/Regenerative dentistry
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| Research Institution | Niigata University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
OKUDA KAZUHIRO 新潟大学, 医歯学系, 准教授 (00169228)
NAGATA MASAKI 新潟大学, 医歯学系, 准教授 (10242439)
TANAKA TAKAAKI 新潟大学, 自然科学系, 教授 (00217043)
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| Project Period (FY) |
2012-04-01 – 2016-03-31
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| Project Status |
Completed (Fiscal Year 2015)
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| Budget Amount *help |
¥18,460,000 (Direct Cost: ¥14,200,000、Indirect Cost: ¥4,260,000)
Fiscal Year 2014: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000)
Fiscal Year 2013: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000)
Fiscal Year 2012: ¥9,490,000 (Direct Cost: ¥7,300,000、Indirect Cost: ¥2,190,000)
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| Keywords | 人工高分子 / スキャホールド / 組織工学 / 骨組織再生 / 生体活性 |
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| Outline of Final Research Achievements |
The purpose of this project is to demonstrate ideal structure and stiffness of scaffolds for osteogenic cells and to develop ideal scaffolds with biodegradable polymer materials. Under regular culture conditions, human periosteal cells (PC) expressed integrin α1β1 and CD44 as major adhesion molecules. Atomospheric plasma treatment modified the titanium surface and facilitated their osteoblastic differentiation. We also found that human platelet-rich fibrin (PRF) extract can be substituted for FBS. As for scaffolding materials, we developed a combinational porous membrane made of polycaprolactone and hydroxyapatite and found its stiffness and microstructure appropriate for PCs.
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Report
(5 results)
Research Products
(41 results)
