Developments of scaffolds and processing technology to maximize the osteogenic potential of cultured periosteal sheets

• Project/Area Number: 24390443
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Dental engineering/Regenerative dentistry
• Research Institution: Niigata University
• Principal Investigator: Kawase Tomoyuki 新潟大学, 医歯学系, 准教授 (90191999)
• Co-Investigator(Kenkyū-buntansha): OKUDA KAZUHIRO 新潟大学, 医歯学系, 准教授 (00169228) ; NAGATA MASAKI 新潟大学, 医歯学系, 准教授 (10242439) ; TANAKA TAKAAKI 新潟大学, 自然科学系, 教授 (00217043)
• Project Period (FY): 2012-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥18,460,000 (Direct Cost: ¥14,200,000、Indirect Cost: ¥4,260,000); Fiscal Year 2014: ¥4,290,000 (Direct Cost: ¥3,300,000、Indirect Cost: ¥990,000); Fiscal Year 2013: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2012: ¥9,490,000 (Direct Cost: ¥7,300,000、Indirect Cost: ¥2,190,000)
• Keywords: 人工高分子 / スキャホールド / 組織工学 / 骨組織再生 / 生体活性

Outline of Final Research Achievements

The purpose of this project is to demonstrate ideal structure and stiffness of scaffolds for osteogenic cells and to develop ideal scaffolds with biodegradable polymer materials. Under regular culture conditions, human periosteal cells (PC) expressed integrin α1β1 and CD44 as major adhesion molecules. Atomospheric plasma treatment modified the titanium surface and facilitated their osteoblastic differentiation. We also found that human platelet-rich fibrin (PRF) extract can be substituted for FBS. As for scaffolding materials, we developed a combinational porous membrane made of polycaprolactone and hydroxyapatite and found its stiffness and microstructure appropriate for PCs.

Research Products

• [Journal Article] Comparative study on angiogenic potential between platelet-rich plasma and platelet-rich fibrin preparations. (2015)
  • Author(s): Kobayashi M, Kawase T, Okuda K, Wolff LF, Yoshie H.
  • Journal Title: Int J Implant Dent Volume: 1 Issue: 1 Pages: 31-31
  • DOI: 10.1186/s40729-015-0032-0
  • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
• [Journal Article] Platelet-rich fibrin membrane combined with beta-tricalcium phosphate for treatment of infrabony defects in chronic periodontitis: a case series. (2015)
  • Author(s): Okuda K, Nakajima Y, Kawase T, Kobayashi M, Kamiya M, Wolff LF, Yoshie H.
  • Journal Title: Jacobs J Dent Res Volume: 2 Pages: 022-022
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Platelet-rich plasma and its derivatives as promising bioactive materials for regenerative medicine: Basic principles and concepts underlying recent advances. (2015)
  • Author(s): Kawase T.
  • Journal Title: Odontology Volume: 103 Issue: 2 Pages: 126-135
  • DOI: 10.1007/s10266-015-0209-2
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Quantitative single-cell motility analysis of platelet-rich plasma-treated endothelial cells in vitro. (2015)
  • Author(s): Kawase T, Tanaka T, Okuda K, Tsuchimochi M, Oda M, Hara T.
  • Journal Title: Cytoskeleton Volume: 72 Issue: 5 Pages: 246-255
  • DOI: 10.1002/cm.21221
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] The heat-compression technique for the conversion of platelet-rich fibrin preparation to a barrier membrane with a reduced rate of biodegradation. (2015)
  • Author(s): Kawase T, Kamiya M, Kobayashi M, Tanaka T, Okuda K, Wolff LF, Yoshie H.
  • Journal Title: J Biomed Mater Res B Applied Biomater Volume: 103B Issue: 4 Pages: 825-831
  • DOI: 10.1002/jbm.b.33262
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] X-ray and UVC irradiation-induced γ-H2AX and p53 formation in normal human periosteal cells in vitro: Markers for quality control in cell therapy. (2015)
  • Author(s): Kawase T, Kamiya M, Hayama K, Nagata M, Okuda K, Yoshie H, Burns DM, Tsuchimochi M, Nakata K.
  • Journal Title: Cytotherapy Volume: 17 Issue: 1 Pages: 112-123
  • DOI: 10.1016/j.jcyt.2014.08.005
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Preparation of poly(L-lactic acid) microfiltration membranes by nonsolvent-induced phase separation method with the aid of surfactants. (2015)
  • Author(s): Minbu H, Ochiaki A, Kawase T, Taniguchi M, LLoyd DR, Tanaka T.
  • Journal Title: J Membr Sci Volume: 479 Pages: 85-94
  • DOI: 10.1016/j.memsci.2015.01.021
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Real-time quantitative polymerase chain reaction and flow cytometric analyses of cell adhesion molecules expressed in human cell-multilayered periosteal sheets in vitro. (2014)
  • Author(s): Kawase T, Uematsu K, Kamiya M, Nagata M, Okuda K, Burns DM, Nakata K, Yoshie H.
  • Journal Title: Cytotherapy Volume: 15 Issue: 5 Pages: 653-661
  • DOI: 10.1016/j.jcyt.2013.11.003
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] An atmospheric-pressure plasma-treated titanium surface potentially supports initial cell adhesion, growth and differentiation of cultured human prenatal-derived osteoblastic cells. (2014)
  • Author(s): Kawase T, Tanaka T, Minbu H, Kamiya M, Oda M, Hara T.
  • Journal Title: J Biomed Mater Res B Volume: in press Issue: 6 Pages: 1289-1296
  • DOI: 10.1002/jbm.b.33114
  • Peer Reviewed
• [Journal Article] Application of stem-cell media to explant culture of human periosteum: An optimal approach for preparing osteogenic cell material. (2013)
  • Author(s): Uematsu K, Nagata M, Kawase T, Suzuki K, Takagi R.
  • Journal Title: J Tissue Eng. Volume: 4:2041731413509646 Pages: 1-12
  • DOI: 10.1177/2041731413509646
  • Peer Reviewed / Open Access
• [Journal Article] Biomechanical evaluation by AFM of cultured human cell-multilayered periosteal sheets. (2013)
  • Author(s): Horimizu M, Kawase T, Tanaka T, Okuda K, Nagata M, Burns DM, Yoshie H.
  • Journal Title: Micron Volume: 48 Pages: 1-10
  • DOI: 10.1016/j.micron.2013.02.001
  • Peer Reviewed
• [Journal Article] Tissue culture of human alveolar periosteal sheets using a stem-cell culture medium(MesenPRO-RS_<TM>) : In vitro expansion of CD146-positive cells and concomitantupregulation of osteogenic potential in vivo. (2013)
  • Author(s): Uematsu K, Kawase T. Nagata M. Suzuki K. Okuda K, Yoshie H. Burns DM, Takagi R.
  • Journal Title: Stem Cell Res Volume: 10 Pages: 1-19
  • Peer Reviewed
• [Journal Article] Microporous membranes of PLLA/PCL blends for periosteal tissue scaffold. (2013)
  • Author(s): Kouya l Tada S, Minbu H, Nakajima Y, Horimizu M, Kawase T, Lloyd DR, Tanaka T
  • Journal Title: Mater Lett Volume: 95 Pages: 103-106
  • DOI: 10.1016/j.matlet.2012.12.076
  • Peer Reviewed
• [Presentation] Stretching processing of hydroxyapatite/poly(ε-caqprolactone)composite scaffolds. (2015)
  • Author(s): Komatsu K, Umemoto S, Minbu H, Kawase T, Tanaka T.
  • Organizer: 2015 International Chemical Congress of Pacific Basin Societies
  • Place of Presentation: Hawaii Convention Center (Honolulu, Hawaii, USA)
  • Year and Date: 2015-12-15
  • Int'l Joint Research
• [Presentation] Quantitative single-cell motility analysis of platelet-rich plasma-treated endothelial cells in vitro. (2015)
  • Author(s): Kawase T, Tanaka T, Okuda K, Tsuchimochi M, Oda M, Hara T.
  • Organizer: 7th WACBE World Congress on Bioengineering
  • Place of Presentation: National University of Singapore (Singapore)
  • Year and Date: 2015-07-06
  • Int'l Joint Research
• [Presentation] 酸化ストレス刺激ヒト骨膜細胞モデルにおけるDNA修復履歴を指標とした品質管理. (2015)
  • Author(s): 川瀬知之、神谷真菜、羽山和秀、永田昌毅、奥田一博、吉江弘正、土持 眞、中田 光.
  • Organizer: 第14回日本再生医療学会
  • Place of Presentation: パシフィコ横浜 (横浜市)
  • Year and Date: 2015-03-19 – 2015-03-21
• [Presentation] Platelet-rich fibrin (PRF)はPlatelet-rich plasma(PRP)と同程度の血管新生効果を有する (2015)
  • Author(s): 小林美登、川瀬知之、奥田一博、佐伯万騎男、吉江弘正.
  • Organizer: 第14回日本再生医療学会
  • Place of Presentation: パシフィコ横浜 (横浜市)
  • Year and Date: 2015-03-19 – 2015-03-21
• [Presentation] 骨膜シート調製における無血清培養に関する基礎的研究. (2015)
  • Author(s): 渡辺真理、藤本陽子、牛木隆志、川瀬知之、奥田一博、永田昌毅、伊藤 彰、吉江弘正、中田 光.
  • Organizer: 第14回日本再生医療学会
  • Place of Presentation: パシフィコ横浜 (横浜市)
  • Year and Date: 2015-03-19 – 2015-03-21
• [Presentation] 固液分離を用いた自家血からの医療用材料の調製. (2014)
  • Author(s): 川瀬知之、田中孝明.
  • Organizer: 化学工学会新潟大会2014
  • Place of Presentation: 新潟大学 (新潟市)
  • Year and Date: 2014-11-22 – 2014-11-23
  • Invited
• [Presentation] 延伸加工したヒドロキシアパタイト複合化ポリカプロラクトン製足場材料. (2014)
  • Author(s): 小松慶子、梅本佐知、民部裕洋、川瀬知之、田中孝明.
  • Organizer: 化学工学会新潟大会2014
  • Place of Presentation: 新潟大学 (新潟市)
  • Year and Date: 2014-11-22 – 2014-11-23
• [Presentation] 界面活性剤を用いて作製したポリ乳酸製多孔質膜の骨芽細胞用細胞培養への応用. (2014)
  • Author(s): 民部裕洋、谷口正之、川瀬知之、田中孝明.
  • Organizer: 化学工学会新潟大会2014
  • Place of Presentation: 新潟大学 (新潟市)
  • Year and Date: 2014-11-22 – 2014-11-23
• [Presentation] The platelet-rich fibrin barrier membrane applicable to GTR treatment: Regulation of its biodegradability by heat-compression. (2014)
  • Author(s): Kawase T, Kamiya M, Kobayashi M, Tanaka T, Okuda K, Wolff LF, Yoshie H.
  • Organizer: 7th World Congress on Preventive and Regenerative Medicine
  • Place of Presentation: Chientan Youth Activity Center (Taipei, Taiwan),
  • Year and Date: 2014-11-05 – 2014-11-07
• [Presentation] 自己血由来多血小板フィブリン膜とβ-TCPによる歯周組織再生効果：症例報告. (2014)
  • Author(s): 奥田一博、中島 悠、小林美登、神谷真菜、堀水 慎、川瀬知之、吉江弘正.
  • Organizer: 第141回日本歯科保存学会2014年秋季学術大会
  • Place of Presentation: 山形テルサ (山形市)
  • Year and Date: 2014-10-30 – 2014-10-31
• [Presentation] Preparation of hydroxyapatite/poly(ε-caprolactone) composite microporous membranes for scaffolds (2014)
  • Author(s): Umemoto S, Komatsu K, Minbu H, Kawase T, Tanaka T
  • Organizer: The 10th International Congress on Membranes and Membrane Processes
  • Place of Presentation: Taihu International Conference Center (Suzuhou, China)
  • Year and Date: 2014-07-20 – 2014-07-25
• [Presentation] An atmospheric-pressure plasma-treated titanium surface potentially supports initial cell adhesion, growth and differentiation of cultured human prenatal-derived osteoblastic cells. (2014)
  • Author(s): Kawase T, Tanaka T, Minbu H, Kamiya M, Oda M, Hara T.
  • Organizer: Termis EU 2014
  • Place of Presentation: Magazzini del Cotone Conference Center (Genova, Italy)
  • Year and Date: 2014-06-10 – 2014-06-13
• [Presentation] The unique structure-function relationship found in osteogenic periosteal sheets. (2014)
  • Author(s): Kawase T, Okuda K, Nagata M, Burns DM, Nakata K, Yoshie H.
  • Organizer: Termis EU 2014
  • Place of Presentation: Magazzini del Cotone Conference Center (Genova, Italy)
  • Year and Date: 2014-06-10 – 2014-06-13
• [Presentation] 自己血由来多血小板フィブリン膜による歯周組織再生効果. (2014)
  • Author(s): 中島 悠、奥田一博、小林美登、神谷真菜、堀水 慎、 川瀬知之、吉江弘正.
  • Organizer: 第57回春季日本歯周病学会学術大会
  • Place of Presentation: 長良川国際会議場 (岐阜市)
  • Year and Date: 2014-05-23 – 2014-05-24
• [Presentation] Platelet-rich fibrin(PRF)との複合化によるヒト培養骨膜シートの骨再生能向上 (2014)
  • Author(s): 堀水 慎、川瀬 知之、久保田 健彦、永田昌毅、奥田 一博、冨田 尊志、両角 俊哉、吉江 弘正.
  • Organizer: 第13回日本再生医療学会
  • Place of Presentation: 国立京都国際会館 (京都市)
• [Presentation] 細胞重層化したヒト培養骨膜シートと単層骨膜細胞シートの細胞接着様式の比較. (2014)
  • Author(s): 川瀬知之、上松晃也、永田昌毅、奥田一博、中田 光、吉江弘正.
  • Organizer: 第13回日本再生医療学会
  • Place of Presentation: 国立京都国際会館 (京都市)
• [Presentation] 培養骨膜シートによる歯周組織・顎骨の再生療法 (2013)
  • Author(s): 吉江弘正、奥田一博、川瀬知之、永田昌毅、高木律夫、中田光
  • Organizer: 第12回日本再生医療学会
  • Place of Presentation: パシフィコ横浜(横浜市)(招待講演)
  • Year and Date: 2013-03-22
• [Presentation] 幹細胞用培地(MesenPRO)は骨膜シート中のCD146+細胞の増加と骨形成ポテンシャルの向Eに貢献する (2013)
  • Author(s): 上松晃也、永田昌毅、川瀬知之、奥田一博、占汪弘正、高木律男
  • Organizer: 第12回日本再生医療学会
  • Place of Presentation: パシフィコ横浜(横浜市)
  • Year and Date: 2013-03-22
• [Presentation] Platelet-rich fibrin(PRF)とヒト培養骨膜シートの複合化による骨形成活性の亢進. (2013)
  • Author(s): 堀水慎、川瀬知之、久保田健彦、永田昌毅、奥田一博、冨田尊志、両角俊哉、吉江弘正
  • Organizer: 第12回日本再生医療学会
  • Place of Presentation: パシフィコ横浜(横浜市)
  • Year and Date: 2013-03-22
• [Presentation] 幹細胞用培地で重層化が促進されたヒト培養骨膜シートの機械的特性と分化抑制との関連性. (2013)
  • Author(s): 川瀬知之、堀水慎、田中孝明、永田昌毅、奥田一博、吉江弘正
  • Organizer: 第12回日本再生医療学会
  • Place of Presentation: パシフィコ横浜(横浜市)
  • Year and Date: 2013-03-22
• [Presentation] ポリ乳酸製多孔質膜を足場として用いた培養骨膜シート. (2013)
  • Author(s): 田中孝明、川瀬知之、西本崇之、奥田一博、永田昌毅、Burns DM、吉江弘正
  • Organizer: 化学工学会第78年会
  • Place of Presentation: 大阪大学豊中キャンパス(豊中市)
  • Year and Date: 2013-03-17
• [Presentation] Platelet-rich fibrin－ヒト培養骨膜シート複合体移植による骨再生能の向上. (2013)
  • Author(s): 堀水 慎、久保田健彦、川瀬知之、永田昌毅、奥田一博、冨田尊志、両角俊哉、吉江弘正.
  • Organizer: 第139回日本歯科保存学会2013年度秋季学術大会
  • Place of Presentation: 秋田県総合生活文化会館 (秋田市)
• [Presentation] Platelet-rich fibrin(PRF)との複合化によるヒト培養骨膜シートの骨形成活性の亢進. (2013)
  • Author(s): 堀水 慎、川瀬知之、久保田健彦、永田昌毅、奥田一博、冨田尊志、両角俊哉、吉江弘正.
  • Organizer: 第56回春季日本歯周病学会学術大会
  • Place of Presentation: タワーホール船堀 (東京都)
• [Presentation] Biological and biomechanical characterization of highly self-multilayered human periosteal sheets as an osteogenic grafting material. (2013)
  • Author(s): Kawase T, Uematsu K, Nagata M, Okuda K, Burns DM, Yoshie H.
  • Organizer: The 19th Annual International Society of Cell Therapy Meeting
  • Place of Presentation: SKYICITY Auckland Convention Centre (New Zealand)
• [Presentation] PLLA/PCLブレンド多孔質膜を足場材料として用いた骨膜組織の培養. (2012)
  • Author(s): 田中孝明、高屋朋彰、多田晉一郎、民部裕洋、中島悠、堀水慎、川瀬知之、凵oyd DR
  • Organizer: 日本バイオマテリアル学会 シンポジウム2012
  • Place of Presentation: 仙台国際センター(仙台市)
  • Year and Date: 2012-11-26
• [Presentation] Preparation of thicker. osteogenic periosteal sheets by xenofree stem-cell media. (2012)
  • Author(s): Uematsu K, Kawase T, Nagata M, Okuda K, Yoshie H
  • Organizer: The 98th Annual Meeting of the American Academy of Periodontology
  • Place of Presentation: Los Angeles Convention Center(米国)
• [Presentation] CD146-positive cells and the osteogenic potential of cultured perioseal sheets. (2012)
  • Author(s): Kawase T, Uematsu K, Nagata M, Okuda K, Yoshie H
  • Organizer: The 98th Annual Meeting of the American Academy of Periodontology
  • Place of Presentation: Los Angeles Convention Center(米国)
• [Presentation] ヒドロキシアパタイト複合化ポリカプロラクトン製足場材料の開発.
  • Author(s): 梅本佐知，民部裕洋，川瀬知之，田中孝明.
  • Organizer: 化学工学会 第45回秋季大会
  • Place of Presentation: 岡山大学 (岡山市)