Establishment of tooth regeneration based on human adult cells for clinical application
| Project/Area Number |
24390447
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Dental engineering/Regenerative dentistry
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| Research Institution | Nihon University |
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Principal Investigator |
HONDA Masaki 日本大学, 歯学部, 准教授 (70361623)
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| Co-Investigator(Kenkyū-buntansha) |
ISOKAWA Keitaro 日本大学, 歯学部, 教授 (50168283)
YUGUCHI Maki 日本大学, 歯学部, 助手 (00256885)
TORIUMI Taku 日本大学, 歯学部, 助教 (40610308)
山崎 洋介 日本大学, 歯学部, 助教 (20366610)
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| Co-Investigator(Renkei-kenkyūsha) |
ARAI Yoshinori 日本大学, 歯学部, 特任教授 (20212607)
WATANABE Nobukazu 東京大学, 医科学研究所, 特任准教授 (10334278)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000)
Fiscal Year 2014: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2013: ¥4,810,000 (Direct Cost: ¥3,700,000、Indirect Cost: ¥1,110,000)
Fiscal Year 2012: ¥7,800,000 (Direct Cost: ¥6,000,000、Indirect Cost: ¥1,800,000)
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| Keywords | 間葉系幹細胞 / 歯髄細胞 / 象牙質再生 / 歯周組織再生 / 成熟脂肪細胞 / 脱分化脂肪細胞 / 担体 / 頬脂肪体 / 歯髄幹細胞 / 歯肉幹細胞 / 細胞移植 / 幹細胞マーカー / 脂肪組織 / フローサイトメーター / 歯髄 / 歯根膜 / 歯の再生 / 歯根 / 再生医学 / 歯周組織 / 臨床応用 / 象牙質塊 / 移植 |
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| Outline of Final Research Achievements |
Mesenchymal stem cells were able to obtain from dental pulp from supernumerary teeth. We examined 10 dental pulp and observed that all dental pulp cells showed the characteristics of dental pulp stem cells. Next, dental pulp cells were seeded into the PLGA scaffold combined with HA. Dentin-like tissue were observed in the scaffold at 16 weeks after implantation.
Dedifferentiated fat (DFAT) cells are known to show the characteristics of mensenchymal stem cells. DFAT cells were implanted into the periodontal defect and exhibited the potential of periodontal tissue engineering. Then, we examined that buccal fat pad is useful for the cell source for DFAT cells. Buccal fat pad-derived DFAT cells showed the similar characteristics of DFAT cells from white adipose tissue from skin. Buccal fat pad is a cell source for DFAT cells and DFAT cells is useful for the periodontal tissue engineering in the future.
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Report
(4 results)
Research Products
(45 results)
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[Journal Article] <b>Characterization of mesenchymal progenitor cells in the crown and root pulp of primary </b><b>teeth </b>2015
Author(s)
Taku TORIUMI, Naoya TAKAYAMA, Miyako MURAKAMI, Momoko SATO, Maki YUGUCHI, Yosuke YAMAZAKI, Koji ETO, Makoto OTSU, Hiromitsu NAKAUCHI, Tetsuo SHIRAKAWA, Keitaro ISOKAWA, Masaki J. HONDA.
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Journal Title
Biomedical Research
Volume: 36
Issue: 1
Pages: 31-45
DOI
NAID
ISSN
0388-6107, 1880-313X
Related Report
Peer Reviewed / Open Access
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[Journal Article] Periodontal tissue regeneration by transplantation of rat adipose-derived stromal cells in combination with PLGA-based solid scaffolds2014
Author(s)
Akita D, Morokuma M, Saito Y, Yamanaka K, Akiyama Y, Sato M, Mashimo T, Toriumi T, Arai Y, kaneko T, Tsukimura N, Isokawa K, Ishigami T, Honda MJ
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Journal Title
Biomedical Research
Volume: 35
Related Report
Peer Reviewed
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[Presentation] Generation of iPSc from root pulp cells of deciduous tooth2014
Author(s)
Toriumi T, Takayama N, Murakami M, Sato M, Yamazaki Y, Yuguchi M, Eto K, Otsu M, Nakauchi H, Shirakawa T, Isokawa K, Honda M.
Organizer
American Association for Dental Research
Place of Presentation
Charlotte, NC, USA
Related Report
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[Presentation] Marute adipocyte-derived cells as a novel tool for periodontal regeneration2014
Author(s)
Akita D, Tsurumachi N, Yamanaka K, Arai Y, Toriumi T, Saito Y, Mashimo T, Sato M, Akiyama Y, Isokawa K, Kaneko T, Matsumoto T, Kano K, Honda M.
Organizer
American Association for Dental Research
Place of Presentation
Charlotte, NC, USA
Related Report
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[Presentation] Enhanced Periodontal Tissue Regeneration by Adipose-Derived Stromal Cell Transplantation2013
Author(s)
AKita D., Arai Y.,Kaneko T.,Yamanaka K.,Mashimo T.,Morokuma M.,Sato M.,Saito Y.,Akiyama Y.,Tsukimura N.,Isokawa K.,Ishigami T.,Honda M
Organizer
World Academy of Science, Engineering and Technology
Place of Presentation
London, UK
Related Report
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[Presentation] Characterization of the Mesenchymal Stem Cells harvested from Human Supernumerary Teeth2013
Author(s)
Sato M, Watanabe N,Watanabe E, Akita D, Akiyama Y, Mashimo T, Toriumi T, Isokawa K, Shirokawa T, Honda M
Organizer
World Academy of Science, Engineering and Technology
Place of Presentation
London, UK
Related Report
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[Patent(Industrial Property Rights)] 歯周組織再生用材料2013
Inventor(s)
本田雅規，秋田大輔，加野浩一郎，松本太郎，金子正
Industrial Property Rights Holder
本田雅規，秋田大輔，加野浩一郎，松本太郎，金子正
Industrial Property Rights Type
特許
Industrial Property Number
2013-119441
Filing Date
2013-06-06
Related Report
