| Budget Amount *help |
¥18,460,000 (Direct Cost: ¥14,200,000、Indirect Cost: ¥4,260,000)
Fiscal Year 2014: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2013: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2012: ¥10,140,000 (Direct Cost: ¥7,800,000、Indirect Cost: ¥2,340,000)
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| Outline of Final Research Achievements |
In this study, we evaluated the expression of miR-1289 in oral cancer tissues and the growth inhibitory effect of miR-1289 in human oral cancer cells. First, we investigated the expression of miR-1289 in oral cancer tissues by real-time quantitative RT-PCR. The expression levels of miR-1289 were significant decreased in oral cancer tissues compared to adjacent normal oral mucosa tissues. Next, synthetic miR-1289 markedly inhibited the growth of human oral cancer cells in vitro and in vivo. Finally, target genes of miR-1289 were explored by microarray and Ingenuity Pathway Analysis (IPA). Microarray and IPA identified 15 genes as targets of miR-1289. In knockdown of these genes, magnesium transporter 1 (MAGT1) showed the most remarkable cell growth inhibition in human oral cancer cells. These results suggest that miR-1289 functions as a novel tumor suppressive microRNA in oral cancer, and may be a useful therapeutic tool for the patients with oral cancer.
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