Expression, function and clinical application of microRNA in oral cancer

• Project/Area Number: 24390457
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 一般
• Research Field: Surgical dentistry
• Research Institution: Ehime University
• Principal Investigator: NAKASHIRO Koichi 愛媛大学, 医学(系)研究科(研究院), 准教授 (90314880)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥18,460,000 (Direct Cost: ¥14,200,000、Indirect Cost: ¥4,260,000); Fiscal Year 2014: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2013: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000); Fiscal Year 2012: ¥10,140,000 (Direct Cost: ¥7,800,000、Indirect Cost: ¥2,340,000)
• Keywords: 口腔癌 / microRNA

Outline of Final Research Achievements

In this study, we evaluated the expression of miR-1289 in oral cancer tissues and the growth inhibitory effect of miR-1289 in human oral cancer cells. First, we investigated the expression of miR-1289 in oral cancer tissues by real-time quantitative RT-PCR. The expression levels of miR-1289 were significant decreased in oral cancer tissues compared to adjacent normal oral mucosa tissues. Next, synthetic miR-1289 markedly inhibited the growth of human oral cancer cells in vitro and in vivo. Finally, target genes of miR-1289 were explored by microarray and Ingenuity Pathway Analysis (IPA). Microarray and IPA identified 15 genes as targets of miR-1289. In knockdown of these genes, magnesium transporter 1 (MAGT1) showed the most remarkable cell growth inhibition in human oral cancer cells. These results suggest that miR-1289 functions as a novel tumor suppressive microRNA in oral cancer, and may be a useful therapeutic tool for the patients with oral cancer.

Research Products

• [Journal Article] Prognostic significance of interleukin-8 and CD163-positive cell-infiltration in tumor tissues in patients with oral squamous cell carcinoma (2014)
  • Author(s): Fujita Y, Okamoto M, Goda H, Tano T, Nakashiro K, Sugita A, Fujita T, Koido S, Homma S, Kawakami Y, Hamakawa H.
  • Journal Title: PLoS ONE Volume: 9 Issue: 12 Pages: e110378-e110378
  • DOI: 10.1371/journal.pone.0110378
  • Peer Reviewed / Open Access
• [Journal Article] Pathophysiology of lung injury induced by common bile duct ligation in mice (2014)
  • Author(s): Shikata F, Sakaue T, Nakashiro K, Okazaki M, Okamura T, Okura M, Ryugo M, Nakamura Y, Yasugi T, Higashiyama S, Izutani H
  • Journal Title: PLoS ONE Volume: 9 Issue: 4 Pages: e94550-e94550
  • DOI: 10.1371/journal.pone.0094550
  • Peer Reviewed / Open Access
• [Journal Article] Immunochemoradiotherapy for patients with oral squamous cell carcinoma: augmentation of OK-432-induced helper T cell 1 response by 5-FU and X-ray irradiation (2013)
  • Author(s): Tano T, Okamoto M, Kan S, Bando T, Goda H, Nakashiro K, Shimodaira S, Koido S, Homma S, Fujita T, Sato M, Yamashita N, Hamakawa H, Kawakami Y
  • Journal Title: Neoplasia Volume: 15 Pages: 805-814
  • Peer Reviewed
• [Journal Article] Targeting Aurora kinase A suppresses the growth of human oral squamous cell carcinoma cells in vitro and in vivo (2013)
  • Author(s): Tanaka H, Nakashiro K, Iwamoto K, Tokuzen N, Fujita Y, Shirakawa R, Oka R, Goda H, Hamakawa H
  • Journal Title: Oral Oncology Volume: 49 Issue: 6 Pages: 551-559
  • DOI: 10.1016/j.oraloncology.2013.02.002
  • Peer Reviewed
• [Journal Article] One-step nucleic acid amplification for detecting lymph node metastasis of head and neck squamous cell carcinoma (2012)
  • Author(s): Goda H, Nakashiro K, et al.
  • Journal Title: Oral Oncology Volume: 48 Pages: 958-63
  • Peer Reviewed
• [Presentation] 口腔癌診断・治療へのmicroRNAの応用 (2014)
  • Author(s): 中城公一、田中宏史、岩本和樹、徳善紀彦、浜川裕之
  • Organizer: 第68回日本口腔科学会学術集会
  • Place of Presentation: 東京
  • Year and Date: 2014-05-07 – 2014-05-09
• [Presentation] 口腔扁平上皮癌における mRNA/microRNA 発現の統合解析 (2014)
  • Author(s): 中城公一
  • Organizer: 第 32 回日本口腔腫瘍学会
  • Place of Presentation: 札幌
• [Presentation] 口腔扁平上皮癌の転移を支持する分子基盤の解明 (2013)
  • Author(s): 中城公一
  • Organizer: 第 50 回日本口腔組織培養学会学術大会
  • Place of Presentation: 東京
• [Presentation] ヒト口腔癌細胞におけるmicroRNA-629の増殖抑制効果 (2012)
  • Author(s): 岩本和樹、中城公一、他
  • Organizer: 第71回日本癌学会学術総会
  • Place of Presentation: 札幌