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Function and regulation of gap junction on oral epithelium organogenesis

Research Project

Project/Area Number 24390460
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypePartial Multi-year Fund
Section一般
Research Field Orthodontic/Pediatric dentistry
Research InstitutionTohoku University

Principal Investigator

Yamada Aya  東北大学, 歯学研究科(研究院), 准教授 (40295085)

Co-Investigator(Kenkyū-buntansha) NAKAMURA TAKASHI  東北大学, 大学院歯学研究科, 准教授 (90585324)
SAITO MASAHIRO  東北大学, 大学院歯学研究科, 教授 (40215562)
SAKAI TAKAYOSHI  大阪大学, 大学院歯学研究科, 教授 (90379082)
IWAMOTO TSUTOMU  徳島大学, 大学院ヘルスバイオサイエンス研究部, 教授 (90346916)
Project Period (FY) 2012-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥18,460,000 (Direct Cost: ¥14,200,000、Indirect Cost: ¥4,260,000)
Fiscal Year 2015: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Fiscal Year 2014: ¥2,600,000 (Direct Cost: ¥2,000,000、Indirect Cost: ¥600,000)
Fiscal Year 2013: ¥3,510,000 (Direct Cost: ¥2,700,000、Indirect Cost: ¥810,000)
Fiscal Year 2012: ¥9,750,000 (Direct Cost: ¥7,500,000、Indirect Cost: ¥2,250,000)
Keywords再生医療 / 細胞間結合 / ギャップ結合 / コネキシン43 / コネキシン43 / 歯髄幹細胞
Outline of Final Research Achievements

Connexin 43 is one of the gap junction protein. To understand the role of connexin 43 (Cx43) in salivary gland development, first we examined the expression of Cx43 by immunostaining using their specific antibody. Cx43 expressed terminal bud epithelum of salivary gland, but not in mesenchymal tisuue. In Cx43 knockout mice, branching morphogenesis of submandibular gland was dramatically inhibited. In presence of inhibitory peptide for Cx43 function, branching morphogenesis of salivary gland organ culture was also inhibited as similar to Cx43 knockout mice. From our results, Cx43 is necessary for salivary gland development and terminal bud branching. Further, gap junctional communication is important for the determination of cell fate. These information is useful for understanding the molecular mechanism of salivary gland development and regeneration.

Report

(5 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Annual Research Report
  • 2013 Annual Research Report
  • 2012 Annual Research Report
  • Research Products

    (22 results)

All 2016 2015 2014 2013 2012 2011 Other

All Int'l Joint Research (2 results) Journal Article (7 results) (of which Int'l Joint Research: 3 results,  Peer Reviewed: 7 results,  Open Access: 5 results,  Acknowledgement Compliant: 1 results) Presentation (13 results) (of which Int'l Joint Research: 2 results)

  • [Int'l Joint Research] 北京大学(中国)

    • Related Report
      2015 Annual Research Report
  • [Int'l Joint Research] 米国国立衛生研究所(米国)

    • Related Report
      2015 Annual Research Report
  • [Journal Article] Mutant GDF5 enhances ameloblast differentiation via accelerated BMP2-induced Smad1/5/8 phosphorylation2016

    • Author(s)
      Liu J, Saito K*, Maruya Y, Nakamura T, Yamada A, Fukumoto E, Ishikawa M, Iwamoto T, Miyazaki K, Yoshizaki K, Ge L, Fukumoto S
    • Journal Title

      Scientific reports

      Volume: 6 Issue: 1 Pages: 23670-23670

    • DOI

      10.1038/srep23670

    • NAID

      120006534957

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Plakophilin-1, a novel Wnt signaling regulator, is critical for tooth development and ameloblast differentiation2016

    • Author(s)
      Miyazaki K, Yoshizaki K, Arai C, Yamada A, Saito K, Ishikawa M, Xue H, Funada K, Haruyama N, Yamada Y, Fukumoto S, Takahashi I
    • Journal Title

      Plos One

      Volume: 11 Issue: 3 Pages: 0152206-0152206

    • DOI

      10.1371/journal.pone.0152206

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Connexin 43 is necessary for salivary gland branching morphogenesis and FGF10-induced ERK1/2 phosphorylation2016

    • Author(s)
      Yamada A, Futagi M, Fukumoto E, Saito K, Yoshizaki K, Ishikawa M, Arakaki M, Hino R, Sugawara Y, Ishikawa M, Naruse M, Miyazaki K, Nakamura T, Fukumoto S.
    • Journal Title

      The Journal of Biological Chemistry

      Volume: 291 Issue: 2 Pages: 904-912

    • DOI

      10.1074/jbc.m115.674663

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Establishment of ex vivo mucocele model using salivary gland organ culture.2014

    • Author(s)
      Hino, R., Futagi, M., Yamada, A., Arakaki, M., Saito, K., Sugawara, Y., Ono, M., Fukumoto, E., Nakamura, T., and Fukumoto, S.
    • Journal Title

      Pediatric dental journal

      Volume: 24(2) Issue: 2 Pages: 78-82

    • DOI

      10.1016/j.pdj.2014.03.003

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] New insights into the functions of enamel matrices in calcified tissues2014

    • Author(s)
      Fukumoto S, Nakamura T, Yamada A, Arakaki M, Saito K, Xu J, Fukumoto E, Yamada Y.
    • Journal Title

      Japanese Dental Science Review

      Volume: 50-2 Issue: 2 Pages: 47-54

    • DOI

      10.1016/j.jdsr.2014.01.001

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access
  • [Journal Article] Establishment of ex vivo mucocele model using salivary gland organ culture2014

    • Author(s)
      Hino Ryoko, Futagi Masaharu, Yamada Aya , Arakaki Makiko, Saito Kan, Sugawara Yu, Ono Mariko, Fukumoto E, Nakamura T, Fukumoto Satoshi
    • Journal Title

      Pediatric Dental Journal

      Volume: in press

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Differentiation of induced pluripotent stem cells into dental mesenchymal cells2011

    • Author(s)
      Otsu, K., et al
    • Journal Title

      Stem Cells Dev.

      Volume: (e-Pub) Issue: 7 Pages: 1156-1164

    • DOI

      10.1089/scd.2011.0210

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Presentation] Induced artificial bone marrow cells from dental pulp cells of primary tooth using a natural compound2015

    • Author(s)
      Aya Yamada, Takashi Nakamura, Makiko Arakaki, Yu Sugawara, Kan Saito, Satoshi Fukumoto
    • Organizer
      25th Congress of the International Association of Paediatric Dentistry
    • Place of Presentation
      Glasgow, Scotland
    • Year and Date
      2015-07-01
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Cross-talk between PDGF-BB and BMP-2 is important for the stemness of dental pulp stem cells2015

    • Author(s)
      Yu Sugawara, Aya Yamada, Satoshi Fukumoto
    • Organizer
      25th Congress of the International Association of Paediatric Dentistry
    • Place of Presentation
      Glasgow, Scotland
    • Year and Date
      2015-07-01
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] Establishment of ex vivo mucocele model using salivary gland organ culture2014

    • Author(s)
      Ryoko Hino, Masaharu Futagi, Aya Yamada, Makiko Arakaki, Kan Saito, Yu Sugawara, Mariko Ono, Emiko Fukumoto, Takashi Nakamura, Satoshi Fukumoto
    • Organizer
      9th BIENNIAL CONFERENCE OF THE PEDIATRIC DENTISTRY ASSOCIATION OF ASIA
    • Place of Presentation
      Singapore
    • Year and Date
      2014-08-23 – 2014-08-24
    • Related Report
      2014 Annual Research Report
  • [Presentation] Mst1/2 regulate dental epithelium proliferation and ameloblast differentiation2014

    • Author(s)
      Masaharu Futagi, Kan Saito, Takashi Nakamura, Aya Yamada, Satoshi Fukumoto
    • Organizer
      92nd International Association for Dental Research
    • Place of Presentation
      Cape Town, South Africa
    • Year and Date
      2014-06-25 – 2014-06-28
    • Related Report
      2014 Annual Research Report
  • [Presentation] Novel Compounds Mimic Hedgehog Activity and Promote Osteoblast Differentiation in C3H10T1/2 Cells and Osteoblastic Cells from Runx2-Deficient Mice2013

    • Author(s)
      Nakamura Takashi, Naruse Masahiro, Ikeuchi Tomoko, Komori Toshihisa, Yamada Aya, Iwamoto Masahiro, Fukumoto Satoshi
    • Organizer
      American Society for Bone and Mineral Research
    • Place of Presentation
      Baltimore,USA
    • Related Report
      2013 Annual Research Report
  • [Presentation] NGF induces cell proliferation in dental epithelial cells via p752013

    • Author(s)
      Ono Mariko, Iwamoto Tsutomu, Arakaki Makiko, Sugawara Yu, Yamada Aya, Saito Kan, Nakamura Takashi, Fukumoto Satoshi
    • Organizer
      2nd Meeting of the International Association for Dental Research-Asia Pacific Region
    • Place of Presentation
      Bangkok,Thailand
    • Related Report
      2013 Annual Research Report
  • [Presentation] 乳歯歯髄を用いた再生医療における課題について2012

    • Author(s)
      二木かおり,山田亜矢,新垣真紀子,岩本 勉,中村卓史,福本 敏
    • Organizer
      第31回日本小児歯科学会中部地方会大会
    • Place of Presentation
      金沢
    • Year and Date
      2012-11-25
    • Related Report
      2012 Annual Research Report
  • [Presentation] Connexin43 regulates TGF-SS-induced ERK1/2 signaling in amelogenesis associated with oculodentodigital dysplasia2012

    • Author(s)
      Yamada Aya, Fukumoto Satoshi
    • Organizer
      8^<th> Biennial Conference, Pediatric Dentistry Association of ASIA
    • Place of Presentation
      Bali, Indonesia
    • Year and Date
      2012-05-26
    • Related Report
      2012 Annual Research Report
  • [Presentation] Filamin-A regulates dental epithelial cell migration in tooth anomaly associated with Mernick-Needles syndrome2012

    • Author(s)
      Miyamoto Ryoko, Yamada Aya, Fukumoto Satoshi
    • Organizer
      8^<th> Biennial Conference, Pediatric Dentistry Association of ASIA
    • Place of Presentation
      Bali, Indonesia
    • Year and Date
      2012-05-26
    • Related Report
      2012 Annual Research Report
  • [Presentation] Pannexin 3, a gap junction protein, regulates odontoblast differentiation2012

    • Author(s)
      Iwamoto Tsutomu, Ishikawa Masaki, Yoshizaki Keigo, Yamada Aya, Nakamura Takashi, Yamada Yoshihiko, Fukumoto Satoshi
    • Organizer
      The ^<th> General Session & Exhibition of the IADR
    • Place of Presentation
      Iguacu Falls, Brazil
    • Related Report
      2012 Annual Research Report
  • [Presentation] ラミニン分子を利用した歯原性上皮細胞の増殖―分化制御

    • Author(s)
      及川知子,二木正晴,山田亜矢,新垣真紀子,宮本綾子,齋藤 幹,中村卓史,福本 敏
    • Organizer
      第51回日本小児歯科学会大会
    • Place of Presentation
      岐阜
    • Related Report
      2013 Annual Research Report
  • [Presentation] 新規エナメル芽細胞マーカーSox21における歯胚分化に対する影響

    • Author(s)
      齋藤 幹,山田亜矢,中村卓史,福本 敏
    • Organizer
      第51回日本小児歯科学会大会
    • Place of Presentation
      岐阜
    • Related Report
      2013 Annual Research Report
  • [Presentation] 唾液腺発生過程におけるConnexin43-FGF10 シグナルの解析

    • Author(s)
      福本 敏,二木正晴,岩本 勉,中村卓史,山田亜矢
    • Organizer
      第51回日本小児歯科学会大会
    • Place of Presentation
      岐阜
    • Related Report
      2013 Annual Research Report

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Published: 2012-04-24   Modified: 2022-01-27  

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