| Project/Area Number |
24390462
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| Research Category |
Grant-in-Aid for Scientific Research (B)
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| Allocation Type | Partial Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Orthodontic/Pediatric dentistry
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| Research Institution | Kyushu University |
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Principal Investigator |
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| Co-Investigator(Kenkyū-buntansha) |
HARUYAMA Naoto 九州大学, 病院, 講師 (70359529)
TERAO Fumie 九州大学, 大学院歯学研究院, 助教 (10510018)
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| Co-Investigator(Renkei-kenkyūsha) |
UMEDA Mariko 九州大学, 病院, 医員 (40707618)
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥18,850,000 (Direct Cost: ¥14,500,000、Indirect Cost: ¥4,350,000)
Fiscal Year 2014: ¥2,860,000 (Direct Cost: ¥2,200,000、Indirect Cost: ¥660,000)
Fiscal Year 2013: ¥2,730,000 (Direct Cost: ¥2,100,000、Indirect Cost: ¥630,000)
Fiscal Year 2012: ¥13,260,000 (Direct Cost: ¥10,200,000、Indirect Cost: ¥3,060,000)
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| Keywords | 歯学 / 細胞・組織 / 再生医療 / 下顎頭軟骨 / 発生・分化 / ストレス / 軟骨細胞 / シグナル伝達 |
|---|
| Outline of Final Research Achievements |
To investigate the mechanism of mechanical stress-induced inhibition of chondrogenic differentiation, we applied the mechanical tensile force to micromass cell culture from mouse embryonic limb bud or mandibular condylar cartilage (MCC). Mechanical stretching enhanced the expression of Caspase-3, and repressed the expression of Caveolin-1, Galectin-3, Patched in MCC micromass culture by antibody array analysis. Immunohistochemical analysis showed up-regulation of Caveolin-1, Galectin-3 and Patched during MCC development. These results indicate that Caveolin-1, Galectin-3 and Patched are associated specifically with as mechanical response of cartilage differentiation in MCC.
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