| Budget Amount *help |
¥18,590,000 (Direct Cost: ¥14,300,000、Indirect Cost: ¥4,290,000)
Fiscal Year 2014: ¥3,900,000 (Direct Cost: ¥3,000,000、Indirect Cost: ¥900,000)
Fiscal Year 2013: ¥3,120,000 (Direct Cost: ¥2,400,000、Indirect Cost: ¥720,000)
Fiscal Year 2012: ¥11,570,000 (Direct Cost: ¥8,900,000、Indirect Cost: ¥2,670,000)
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| Outline of Final Research Achievements |
An asymmetric cell division is crucial for the pool of the tissue stem cells and homeostasis of the organs. To dissect role of the asymmetric stem-cell divisions in the periodontal tissue, we first established the induction model for the senescence in human periodontal ligament (HPDL) cells in the primary culture. HPDL cells showed the decreased ratio of the SSEA3/4,ES cell marker, positive subpopulations depends on the progression of the cell dividing. Trans OMICS approach involving the analysis of transcriptome and proteome identified the global change of profiles in extra cellular matrix (ECM) protein species for the stem-cells exhaustion in senescent HPDL cells. We suggest the proper ECM synthesis is indispensable for the cellular communications in the stem cells/ niches in the homeostasis of periodontal tissue.
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