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Estimation of sources of antibiotic resistant bacteria and analysis of their aquired resistance in Southeast Asian water environment

Research Project

Project/Area Number 24404017
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypePartial Multi-year Fund
Section海外学術
Research Field Civil and environmental engineering
Research InstitutionYamagata University

Principal Investigator

WATANABE Toru  山形大学, 農学部, 准教授 (10302192)

Co-Investigator(Kenkyū-buntansha) HONDA Ryo  金沢大学, 理工研究域, 助教 (40422456)
真砂 佳史  東北大学, 工学研究科, 助教 (50507895)
Co-Investigator(Renkei-kenkyūsha) MASAGO Yoshifumi  東北大学, 未来科学技術共同研究センター, 准教授 (50507895)
Research Collaborator WILAI Chiemchaisri  カセサート大学(タイ王国), 工学部, 准教授
CHART Chiemchaisri  カセサート大学(タイ王国), 工学部, 准教授
Project Period (FY) 2012-04-01 – 2015-03-31
Project Status Completed (Fiscal Year 2014)
Budget Amount *help
¥17,940,000 (Direct Cost: ¥13,800,000、Indirect Cost: ¥4,140,000)
Fiscal Year 2014: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2013: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Fiscal Year 2012: ¥7,800,000 (Direct Cost: ¥6,000,000、Indirect Cost: ¥1,800,000)
Keywords薬剤耐性菌 / 大腸菌 / 水環境 / 耐性遺伝子 / 下水処理 / 病院排水 / 国際研究者交流 / タイ王国 / タイ / 抗生物質 / 水圏現象 / 微生物 / 東南アジア / テトラサイクリン / 下水処理場 / チャオプラヤ川
Outline of Final Research Achievements

1) Twenty-four kinds of resistance genes were detected from isolates of Escherichia coli (n=316) from the Chao Phraya River in Thailand. Detection rates of resistance genes to penicillins and tetracyclines were relatively high. Phylogenetic analysis revealed that these isolates were categorized into five clusters. We could not find significant differences in detection rates of any antibiotic resistance genes among the clusters, indicating the difficulty to estimate origins of the resistance genes carried by E. coli based on their phylogenetic relationship.
2) We conducted an experiment to treat synthetic municipal wastewater using bench-scale reactor of activated sludge process in Thailand. It demonstrated the increase in resistant rate to norfloxacin (NOR) of E. coli in the reactor when NOR was added at a high concentration as reported in hospital wastewater. We also found that the NOR resistance of E. coli appeared to be induced by a low concentration of NOR (<50 ng/L) in river water.

Report

(4 results)
  • 2014 Annual Research Report   Final Research Report ( PDF )
  • 2013 Annual Research Report
  • 2012 Annual Research Report
  • Research Products

    (11 results)

All 2015 2014 2013 2012

All Journal Article (1 results) (of which Peer Reviewed: 1 results,  Acknowledgement Compliant: 1 results) Presentation (10 results)

  • [Journal Article] Effect of norfloxacin on antibiotic resistance of Eschericia coli: Comparison between sequencing batch reactor and sequencing batch membrane bioreactor2015

    • Author(s)
      Wilai Chiemchaisri, Phanida Deerut, Toru Watanabe, Ryo Honda, Chart Chiemchaisri
    • Journal Title

      ASEAN Engineering Journal Part C

      Volume: 4(1) Pages: 81-92

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Acknowledgement Compliant
  • [Presentation] チャオプラヤ川流域に生息する薬剤耐性大腸菌の起源推定のための系統発生解析2015

    • Author(s)
      小澤耕平,渡辺幸三,本多了,梶原晶彦,渡部徹
    • Organizer
      平成26年度土木学会東北支部技術研究発表会
    • Place of Presentation
      宮城県多賀城市
    • Year and Date
      2015-03-07
    • Related Report
      2014 Annual Research Report
  • [Presentation] Restriction fragment length polymorphism (RFLP) analysis of antibiotic resistant Escherichia coli for its source tracking2014

    • Author(s)
      Kohei Ozawa, Toru Watanabe, Horoaki Ito, Ryo Honda, Wilai Chiemchaisri and Kozo Watanabe
    • Organizer
      The 7th ASEAN Environmental Engineering Conference
    • Place of Presentation
      Puerto Princesa, Palawan, Philippines
    • Year and Date
      2014-11-21 – 2014-11-22
    • Related Report
      2014 Annual Research Report
  • [Presentation] Impacts of final sedimentation process on profile of quinolone-resistance genesand their experssion in Escherichia coli isolates from a wastewater treatment process2014

    • Author(s)
      Chihiro Tachi, Ryo Honda, Eri Tsuchiya-Nakakahira, Tingting Gu, Ryoko Yamamoto-Ikemoto, Toru Watanabe
    • Organizer
      Water and Environment Technology Conference 2014
    • Place of Presentation
      Tokyo, Japan
    • Year and Date
      2014-06-28 – 2014-06-29
    • Related Report
      2014 Annual Research Report
  • [Presentation] チャオプラヤ川流域から分離された大腸菌のテトラサイクリン耐性遺伝子2013

    • Author(s)
      小澤耕平
    • Organizer
      平成24年度土木学会東北支部技術研究発表会
    • Place of Presentation
      東北大学(宮城県)
    • Year and Date
      2013-03-09
    • Related Report
      2012 Annual Research Report
  • [Presentation] 水環境から分離された大腸菌のテトラサイクリン耐性遺伝子の検出2013

    • Author(s)
      小澤耕平
    • Organizer
      第18回庄内・社会基盤技術フォーラム
    • Place of Presentation
      東北公益文科大学(山形県)
    • Year and Date
      2013-01-24
    • Related Report
      2012 Annual Research Report
  • [Presentation] Tetracycline resistance genes detected in Escherichia coli isolated from the Chao Phraya River and its tributaries in Thailand2013

    • Author(s)
      K. Ozawa, T. Watanabe, H. Ito, Y. Masago, W. Chiemchaisri, R. Honda and A. Kajihara
    • Organizer
      The 5th IWA-ASPIRE Conference and Exhibition
    • Place of Presentation
      Daejeon, Korea
    • Related Report
      2013 Annual Research Report
  • [Presentation] Geographical distribution of tetracycline resistance genes carried by Esherichia coli in the Chao Phraya river and its tributaries2013

    • Author(s)
      K. Ozawa, H. Ito, T. Watanabe, R. Honda, Y. Masago, W. Chiemchaisri
    • Organizer
      The International Conference on the Preservation and Rehabilitation of Urban Water Environment for Asian Core Program of NRCT, JSPS and ERDT
    • Place of Presentation
      Bangkok, Thailand
    • Related Report
      2013 Annual Research Report
  • [Presentation] Change in antibiotic resistance of Escherichia coli during domestic wastewater treatment: comparison between sequencing batch reactor (SBR) and sequencing batch membrane bioreactor (SB-MBR)2013

    • Author(s)
      T. Phonsin, W. Chiemchaisri, C. Chiemchaisri, T. Watanabe, R. Honda
    • Organizer
      The International Conference on the Preservation and Rehabilitation of Urban Water Environment for Asian Core Program of NRCT, JSPS and ERDT
    • Place of Presentation
      Bangkok, Thailand
    • Related Report
      2013 Annual Research Report
  • [Presentation] チャオプラヤ川流域から分離された大腸菌からのテトラサイクリン耐性遺伝子の検出2013

    • Author(s)
      小澤耕平,渡部徹,伊藤紘晃,真砂佳史,Chiemchaisri Wilai,本多了
    • Organizer
      土木学会第68回年次学術講演会
    • Place of Presentation
      習志野市
    • Related Report
      2013 Annual Research Report
  • [Presentation] Municipal wastewater treatment plants as a potential source of antibiotic resistant bacteria in southeast Asia2012

    • Author(s)
      Toru Watanabe
    • Organizer
      The 3rd Seminar on Asian Water Environment
    • Place of Presentation
      Windsor Plaza Hotel (HOChi Minh City, Vietnam)
    • Related Report
      2012 Annual Research Report

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Published: 2012-04-24   Modified: 2019-07-29  

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