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Methanotrophic food webs and carbon recycling in a subtrophic lake

Research Project

Project/Area Number 24405007
Research Category

Grant-in-Aid for Scientific Research (B)

Allocation TypePartial Multi-year Fund
Section海外学術
Research Field Ecology/Environment
Research InstitutionResearch Institute for Humanity and Nature (2014-2015)
Kyoto University (2012-2013)

Principal Investigator

OKUDA Noboru  総合地球環境学研究所, 研究部, 准教授 (30380281)

Co-Investigator(Kenkyū-buntansha) KOJIMA Hisaya  北海道大学, 低温科学研究所, 助教 (70400009)
中野 伸一  京都大学, 生態学研究センター, 教授 (50270723)
陀安 一郎  京都大学, 生態学研究センター, 准教授 (80353449)
Co-Investigator(Renkei-kenkyūsha) FUKUI Manabu  北海道大学, 低温科学研究所, 教授 (60305414)
Tayasu Ichiro  総合地球環境学研究所, 研究部, 教授 (80353449)
NAKANO Shin-ichi  京都大学, 生態学研究センター, 教授 (50270723)
Research Collaborator ITOH Masayuki  京都大学, 東南アジア研究所, 助教
KOBAYASHI Yuki  山口大学, 大学院医学系研究科, 講師
FUJIBAYASHI Megumu  秋田県立大学, 生物資源科学部, 助教
SHIAH Fuh-Kwo  中央研究院, 環境変遷研究中心, 研究員
HSIEH Chih-hao  國立台灣大學, 海洋研究所, 副教授
MIKI Takeshi  國立台灣大學, 海洋研究所, 副教授
HO Pei-Chi  中央研究院, 環境変遷研究中心, 大学院生
CHANG Chun-Wei  中央研究院, 環境変遷研究中心, 大学院生
Project Period (FY) 2012-04-01 – 2016-03-31
Project Status Completed (Fiscal Year 2015)
Budget Amount *help
¥15,860,000 (Direct Cost: ¥12,200,000、Indirect Cost: ¥3,660,000)
Fiscal Year 2014: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
Fiscal Year 2013: ¥6,630,000 (Direct Cost: ¥5,100,000、Indirect Cost: ¥1,530,000)
Fiscal Year 2012: ¥5,070,000 (Direct Cost: ¥3,900,000、Indirect Cost: ¥1,170,000)
Keywordsメタン酸化細菌 / メタン栄養食物網 / 炭素リサイクル / 嫌気的メタン酸化 / NC10 / 安定同位体混合モデル / 脂肪酸分析 / 反応-拡散-移流モデル / NC10 / 炭素フラックスモデル / 表層-底層カップリング / CARD-FISH法 / 分子微生物学解析 / 湖沼炭素循環 / Proteobacteria門 / CARD-FISH / 冬季鉛直混合
Outline of Final Research Achievements

Recent studies suggest that freshwaters are the primary source of atmospheric methane emitted from natural ecosystems. Methane oxidizing bacteria (MOB) which assimilate dissolved methane aerobically or anaerobically have great impacts on methane cycling in freshwater ecosystems. Methanotrophic food webs (MFWs), in which methane-derived carbon is trophically transferred through the MOB and embedded within a food web, serve as a carbon recycling unit in lake ecosystems. In a deep subtropical Fei-Tsui Reservoir which is located in Taipei, methane profiles and MOB community composition showed characteristics intermediate between subtropical monomictic and tropical meromictic lakes. Our four-year monitoring revealed that typhoon-induced disturbance of thermal stratification and the subsequent decrease in profundal methane storage due to methane oxidation can be the primary limno-physical mechanism to affect the relative contribution of MFWs to the overall trophic carbon flows.

Report

(5 results)
  • 2015 Annual Research Report   Final Research Report ( PDF )
  • 2014 Annual Research Report
  • 2013 Annual Research Report
  • 2012 Annual Research Report
  • Research Products

    (43 results)

All 2016 2015 2014 2013 2012 Other

All Int'l Joint Research (1 results) Journal Article (14 results) (of which Int'l Joint Research: 2 results,  Peer Reviewed: 14 results,  Open Access: 3 results,  Acknowledgement Compliant: 2 results) Presentation (22 results) (of which Int'l Joint Research: 2 results,  Invited: 2 results) Book (5 results) Remarks (1 results)

  • [Int'l Joint Research] 中央研究院環境変遷研究中心/國立台灣大學海洋研究所(台湾)

    • Related Report
      2015 Annual Research Report
  • [Journal Article] Fish diversity and trophic interactions in Lake Sampaloc (Luzon Is., Philippines)2016

    • Author(s)
      Briones, J. C. A., R. D. S. Papa, G. A. Cauyan, N. Mendoza & N. Okuda
    • Journal Title

      Tropical Ecology

      Volume: 57 Pages: 559-566

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research
  • [Journal Article] Effect of interannual variation in winter vertical mixing on CH4 dynamics in a subtropical reservoir2016

    • Author(s)
      Itoh, M., Y. Kobayashi, T.-Y. Chen, T. Tokida, M. Fukui, H. Kojima, T. Miki, I. Tayasu, F.-K. Shiah & N. Okuda
    • Journal Title

      Journal of Geophysical Research: Biogeosciences

      Volume: 120 Issue: 7 Pages: 1246-1261

    • DOI

      10.1002/2015jg002972

    • Related Report
      2015 Annual Research Report
    • Peer Reviewed / Open Access / Int'l Joint Research / Acknowledgement Compliant
  • [Journal Article] Changes in bacterial community structure associated with phytoplankton succession in outdoor experimental ponds2015

    • Author(s)
      Kobayashi, Y., Y. Hodoki, K. Ohbayashi, N. Okuda, & S. Nakano
    • Journal Title

      Plankton and Benthos Research

      Volume: 10 Pages: 34-44

    • NAID

      130004946447

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Upwelling-like bottom intrusion enhances the pelagic-benthic coupling by a fish predator in a coastal food web2014

    • Author(s)
      Hamaoka, H., A. Kaneda, N. Okuda & K. Omori
    • Journal Title

      Aquatic Ecology

      Volume: 48 Issue: 1 Pages: 63-71

    • DOI

      10.1007/s10452-013-9466-8

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Community structure of planktonic methane-oxidizing bacteria in a subtropical reservoir characterized by dominance of phylotype closely related to nitrite reducer2014

    • Author(s)
      Kojima, H., R. Tokizawa, K. Kogure, Y. Kobayashi, M. Itoh, N. Okuda, F.-K. Shiah & M. Fukui
    • Journal Title

      Scientific Reports

      Volume: 4 Issue: 1 Pages: 5728-5728

    • DOI

      10.1038/srep05728

    • NAID

      120005463907

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed / Open Access / Acknowledgement Compliant
  • [Journal Article] Food habits and distribution of the Lake Taal sea snake (Hydrophis semperi Garman, 1881) and the sympatric little file snake (Acrochordus granulatus Schneider, 1799) in Lake Taal, Philippines2014

    • Author(s)
      Garcia, V. O. S., R. D. S. Papa, J. C. A. Briones, N. Mendoza, N. Okuda & A. C. Diesmos
    • Journal Title

      Asian Herpetological Research

      Volume: 5 Pages: 255-262

    • Related Report
      2014 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Anatoxin-a-producing Raphidiopsis mediterranea Skuja var. grandis Hill is one ecotype of non-heterocystous Cuspidothrix issatschenkoi (Usacev) Rajaniemi et al. in Japanese lakes.2013

    • Author(s)
      Hodoki, Y., Ohbayashi, K., Kobayashi, Y., Takasu, H., Okuda, N., Nakano, S.
    • Journal Title

      Harmful Algae

      Volume: 21-22 Pages: 44-53

    • DOI

      10.1016/j.hal.2012.11.007

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Genotypic composition and the relationship between genotypic composition and geographical proximity of the cyanobacterium Microcystis aeruginosa in western Japan2013

    • Author(s)
      Ohbayashi K., Y. Hodoki, Y. Kobayashi, N. Okuda & S. Nakano
    • Journal Title

      Canadian Journal of Microbiology

      Volume: 59 Issue: 4 Pages: 266-272

    • DOI

      10.1139/cjm-2012-0724

    • NAID

      120005244792

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Grazing impact on the cyanobacterium Microcystis aeruginosa by the heterotrophic flagellate Collodictyon triciliatum in an experimental pond2013

    • Author(s)
      Kobayashi, Y., Y. Hodoki, K. Ohbayashi, N. Okuda, & S. Nakano
    • Journal Title

      Limnology

      Volume: 14 Issue: 1 Pages: 43-49

    • DOI

      10.1007/s10201-012-0384-6

    • NAID

      10031147632

    • Related Report
      2013 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Seasonal and site-specific variability in terrigenous particulate organic carbon concentration in near-shore waters of Lake Biwa2013

    • Author(s)
      Sakai, Y., Karube, Z., Takeyama, T., Kohzu, A., Yoshimizu, C., Nagata, T., Tayasu, I. and Okuda, N
    • Journal Title

      Japan. Limnology

      Volume: 14 Issue: 2 Pages: 167-177

    • DOI

      10.1007/s10201-012-0394-4

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 生物代謝のスケールアップ : 個体から生態系へ2013

    • Author(s)
      福森香代子・奥田昇
    • Journal Title

      日本生態学会誌

      Volume: 63 Pages: 113-123

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Development of nine markers and characterization of the microsatellite loci in the endangered Gymnogobius isaza (Gobiidae)2012

    • Author(s)
      Araki K. S., T. Nakazawa, A. Kawakita, H. Kudoh & N. Okuda
    • Journal Title

      International Journal of Molecular Sciences

      Volume: 13 Pages: 5700-5705

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] Long-term changes in the diet of Gymnogobius isaza from Lake Biwa, Japan: effects of body size and environmental prey availability2012

    • Author(s)
      Briones J. C.
    • Journal Title

      PLoS ONE

      Volume: 7 Issue: 12 Pages: e53167-e53167

    • DOI

      10.1371/journal.pone.0053167

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Journal Article] 安定同位体を用いた水田生態系の構造と機能の評価手法,2012

    • Author(s)
      奥田昇
    • Journal Title

      日本生態学会誌

      Volume: 62 Pages: 207-215

    • Related Report
      2012 Annual Research Report
    • Peer Reviewed
  • [Presentation] Methanotrophic food webs as a carbon recycling system in lakes under climate changes2016

    • Author(s)
      Okuda, N.
    • Organizer
      The 1st Philippine Symposium on Freshwater Biodiversity and Ecosystems
    • Place of Presentation
      University of Santo Tomas, Manila
    • Year and Date
      2016-06-07
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research / Invited
  • [Presentation] 生物多様性が駆動する栄養循環と流域圏社会-生態システムの健全性2016

    • Author(s)
      奥田昇
    • Organizer
      第63回日本生態学会
    • Place of Presentation
      仙台国際センター、仙台
    • Year and Date
      2016-03-24
    • Related Report
      2015 Annual Research Report
  • [Presentation] 地球温暖化とそれに伴う湖底の貧酸素化が琵琶湖深水層のベントス群集に与える影響2016

    • Author(s)
      酒井陽一郎・小板橋忠俊・柴田淳也・岡野淳一・谷内茂雄・中野伸一・奥田昇
    • Organizer
      第63回日本生態学会
    • Place of Presentation
      仙台国際センター、仙台
    • Year and Date
      2016-03-24
    • Related Report
      2015 Annual Research Report
  • [Presentation] Unusual stable isotope ratio in parasites2015

    • Author(s)
      Urabe, M., E. Kamiya & N. Okuda
    • Organizer
      International Conference Dedicated to the 20th Anniversary of CNEAS
    • Place of Presentation
      Sendai International Center, Sendai
    • Year and Date
      2015-12-05
    • Related Report
      2015 Annual Research Report
    • Int'l Joint Research
  • [Presentation] 日本に分布する有毒ラン藻とその環境特性2015

    • Author(s)
      程木義邦・大林夏湖・小林由紀・高巣裕之・奥田昇・中野伸一
    • Organizer
      日本微生物生態学会第30回大会
    • Place of Presentation
      土浦市役所
    • Year and Date
      2015-10-17
    • Related Report
      2015 Annual Research Report
  • [Presentation] 富栄養化と温暖化が沖帯ベントス群集に与える影響の評価2015

    • Author(s)
      酒井陽一郎・小板橋忠俊・柴田淳也・谷内茂雄・中野伸一・奥田昇
    • Organizer
      第79回日本陸水学会
    • Place of Presentation
      北海道大学(函館)
    • Year and Date
      2015-09-27
    • Related Report
      2015 Annual Research Report
  • [Presentation] リン酸‐酸素安定同位体分析が拓くリン循環研究の黎明2015

    • Author(s)
      奥田 昇・Abigail P. Cid・陀安一郎・井手淳一郎
    • Organizer
      日本地球惑星科学連合2015年大会
    • Place of Presentation
      幕張
    • Year and Date
      2015-05-24
    • Related Report
      2014 Annual Research Report
  • [Presentation] What’s the methanotrophic food web?2015

    • Author(s)
      Okuda, N.
    • Organizer
      The 62nd ESJ Annual Meeting
    • Place of Presentation
      Kagoshima
    • Year and Date
      2015-03-21
    • Related Report
      2014 Annual Research Report
  • [Presentation] CH4 dynamics in a subtropical reservoir under climate changes2015

    • Author(s)
      Itoh, M.
    • Organizer
      The 62nd ESJ Annual Meeting
    • Place of Presentation
      Kagoshima
    • Year and Date
      2015-03-21
    • Related Report
      2014 Annual Research Report
  • [Presentation] Vertical and seasonal profiles of methane oxidizing bacteria (MOB) in FTR2015

    • Author(s)
      Kobayashi, Y.
    • Organizer
      The 62nd ESJ Annual Meeting
    • Place of Presentation
      Kagoshima
    • Year and Date
      2015-03-21
    • Related Report
      2014 Annual Research Report
  • [Presentation] Methanotrophic food webs based on fatty acid biomarker2015

    • Author(s)
      Fujibayashi, M.
    • Organizer
      The 62nd ESJ Annual Meeting
    • Place of Presentation
      Kagoshima
    • Year and Date
      2015-03-21
    • Related Report
      2014 Annual Research Report
  • [Presentation] Summer hypoxia determines the coupling of methanotrophic and pelagic foodweb2015

    • Author(s)
      Ho, P.-C.
    • Organizer
      The 62nd ESJ Annual Meeting
    • Place of Presentation
      Kagoshima
    • Year and Date
      2015-03-21
    • Related Report
      2014 Annual Research Report
  • [Presentation] Intraspecific differences in vertical habitat and food utilization by crustacean zooplankton: stable isotopic evidence2014

    • Author(s)
      Sakai, Y. & N. Okuda
    • Organizer
      ISRLE2014
    • Place of Presentation
      Chuncheon, Korea
    • Year and Date
      2014-08-25
    • Related Report
      2014 Annual Research Report
  • [Presentation] Toward the evaluation of extinction risk of Lake Biwa benthic species due to global warming2014

    • Author(s)
      Yachi, S., D. Kitazawa, S. Nakano, Y. Sakai & N. Okuda
    • Organizer
      JSMB/SMB 2014
    • Place of Presentation
      Osaka
    • Year and Date
      2014-07-29
    • Related Report
      2014 Annual Research Report
  • [Presentation] Methanotrophic food webs as a carbon recycling system in lakes under climate changes April 2014, Haikou2014

    • Author(s)
      Okuda, N., I. Tayasu, S. Nakano, M. Ito, M. Fukui, H. Kojima, K. Kogure, M. Fujibayashi, C. Maruo, P.-C. Ho, C.-W. Chang, L. Zhang, W.-H. Teng, T. Miki, C.-H. Hsieh, Y. Kobayashi, C.-C. Chang & F.-K. Shiah
    • Organizer
      The 6th EAFES International Congress
    • Place of Presentation
      Haikou,China
    • Year and Date
      2014-04-11
    • Related Report
      2014 Annual Research Report
  • [Presentation] 温暖化と富栄養化に伴う湖底貧酸素化に対する底生種の絶滅リスク評価2014

    • Author(s)
      谷内茂雄・奥田 昇・酒井陽一郎・北澤大輔・中野伸一
    • Organizer
      第61回日本生態学会
    • Place of Presentation
      広島国際会議場(広島市)
    • Related Report
      2013 Annual Research Report
  • [Presentation] 標本が拓く魚類学の無限の可能性:琵琶湖の過去・現在・未来2013

    • Author(s)
      奥田昇
    • Organizer
      第46回日本魚類学会
    • Place of Presentation
      宮崎観光ホテル(宮崎市)
    • Related Report
      2013 Annual Research Report
  • [Presentation] 大津臨湖実験所の遺産:生物標本が語る琵琶湖100 年史2013

    • Author(s)
      奥田昇
    • Organizer
      第78回日本陸水学会
    • Place of Presentation
      龍谷大学大津キャンパス(大津市)
    • Related Report
      2013 Annual Research Report
    • Invited
  • [Presentation] Spatio-temporal dynamics of aerobic methane oxidizing bacterial community and its”methanotrophic pathways in the pelagic food web of subtropical Fei-Tsui Reservoir2013

    • Author(s)
      Kobayashi, Y., M. Ito, H. Kojima, C.-H. M. Lin, K. Kuo, K.-W. Wang, M. Fukui, F.-K. Shiah & N. Okuda
    • Organizer
      13th Symposium on Aquatic Microbial Ecology
    • Place of Presentation
      Stresa Congress Centre, Stresa City, Italy
    • Related Report
      2013 Annual Research Report
  • [Presentation] Intraspecific differences in vertical habitat utilization by crustacean zooplankton : stable isotopic evidence2012

    • Author(s)
      Sakai, Y. & N. Okuda
    • Organizer
      2012 ASLO Aquatic Sciences Meeting
    • Place of Presentation
      Biwako Hall, shiga, Japan
    • Year and Date
      2012-07-12
    • Related Report
      2012 Annual Research Report
  • [Presentation] Biological specimens tell us a centurial history of ecosystem alterations in the ancient Lake Biwa2012

    • Author(s)
      Okuda N., Y. Kato, T. Komiya, Y. Okuzaki, M. Hori, I. Tayasu & T. Nagata
    • Organizer
      2012 ASLO Aquatic Sciences Meeting
    • Place of Presentation
      Biwako Hall, shiga, Japan
    • Year and Date
      2012-07-11
    • Related Report
      2012 Annual Research Report
  • [Presentation] Long-term changes in the diet and intestinal parasite fauna of Gymnogobius isaza from Lake Biwa, Japan : Effects of body size and prey availability2012

    • Author(s)
      Briones, J. A., C. Tsai, T. Nakazawa, Y. Sakai, M. Urabe, R. Papa, C. Hsieh, N. Okuda
    • Organizer
      2012 ASLO Aquatic Sciences Meeting
    • Place of Presentation
      Biwako Hall, shiga, Japan
    • Year and Date
      2012-07-09
    • Related Report
      2012 Annual Research Report
  • [Book] 琵琶湖は呼吸する2015

    • Author(s)
      熊谷道夫・浜端悦治・奥田昇
    • Total Pages
      180
    • Publisher
      海鳴社
    • Related Report
      2015 Annual Research Report
  • [Book] 湖沼近過去調査法2014

    • Author(s)
      仲澤剛史・奥田昇 (占部城太郎編著)
    • Total Pages
      236
    • Publisher
      共立出版
    • Related Report
      2014 Annual Research Report
  • [Book] Biodiversity in aquatic systems and environments: Lake Biwa2013

    • Author(s)
      Okuda, N., K. Watanabe, K. Fukumori, S. Nakano & T. Nakazawa
    • Total Pages
      91
    • Publisher
      Springer Japan
    • Related Report
      2013 Annual Research Report
  • [Book] Predator diversity changes the world: from gene to ecosystem. In: Biodiversity in aquatic systems and environments: Lake Biwa2013

    • Author(s)
      Okuda, N. & K. Fukumori
    • Publisher
      Springer Japan
    • Related Report
      2013 Annual Research Report
  • [Book] A food web and its long-term dynamics in Lake Biwa : a stable isotope approach in : Lake Biwa : Interactions between Nature and People2012

    • Author(s)
      Okuda, N., T. Takeyama, T. Komiya, Y. Kato, Y. Okuzaki, Z. Karube, Y. Sakai, M, Hori, I. Tayasu & T. Nagata
    • Publisher
      Springer
    • Related Report
      2012 Annual Research Report
  • [Remarks] 奥田昇

    • URL

      http://www.ecology.kyoto-u.ac.jp/~nokuda/index.html

    • Related Report
      2013 Annual Research Report

URL: 

Published: 2012-04-24   Modified: 2022-02-16  

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