Study on genetic diversity and evaluation of genetic resources of Haskap in Eurasia and North America

• Project/Area Number: 24405022
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 海外学術
• Research Field: Horticulture/Landscape architecture
• Research Institution: Hokkaido University
• Principal Investigator: HOSHINO Yoichiro 北海道大学, 北方生物圏フィールド科学センター, 准教授 (50301875)
• Project Period (FY): 2012-04-01 – 2017-03-31
• Project Status: Completed (Fiscal Year 2016)
• Budget Amount: ¥15,470,000 (Direct Cost: ¥11,900,000、Indirect Cost: ¥3,570,000); Fiscal Year 2015: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2014: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2013: ¥3,770,000 (Direct Cost: ¥2,900,000、Indirect Cost: ¥870,000); Fiscal Year 2012: ¥4,160,000 (Direct Cost: ¥3,200,000、Indirect Cost: ¥960,000)
• Keywords: 植物遺伝資源 / 遺伝的多様性 / 系統解析 / 遺伝資源 / 分子マーカー / ハスカップ / 系統分化 / 遺伝資源・系統分化 / 遺伝子資源 / 国際情報交換 / 中国

Outline of Final Research Achievements

Genetic diversity using DNA markers and ploidy variation as well as DNA content variation by flow cytometry were analyzed for Haskap (Lonicera caerulea). Analysis of the genetic resources in northeast China and Russia revealed that the Chinese accessions were diploid, and Russian accessions were diploid and tetraploid. Unexpected pentaploid was found in Russian accession. As a result of polymorphism analysis by AFLP markers, clusters reflecting geographical distribution and classification of species were obtained. In addition to the analysis of the chloroplast genome, it was considered that the genetic resources of northeastern China and Far East Russia include high genetic diversity.

Research Products

• [Int'l Joint Research] バビロフ研究所(ロシア連邦)
• [Int'l Joint Research] バビロフ研究所(ロシア連邦)
• [Journal Article] Assessment of genetic relationships among cultivated and wild Rubusaccessions using AFLP markers (2015)
  • Author(s): Tomoya Miyashita, Hisato Kunitake, Norishige Yotsukura, Yoichiro Hoshino
  • Journal Title: Scientia Horticulturae Volume: 193 Pages: 165-173
  • DOI: 10.1016/j.scienta.2015.07.004
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Interploid and intraploid hybridizations to produce polyploid Haskap (Lonicera caerulea var. emphyllocalyx) plants (2015)
  • Author(s): Miyashita T, Hoshino Y
  • Journal Title: Euphytica Volume: 201 Issue: 1 Pages: 15-27
  • DOI: 10.1007/s10681-014-1159-4
  • NAID: 120005679510
  • Peer Reviewed
• [Presentation] ハスカップの二倍体野生集団の果実特性と遺伝的多様性の解析 (2016)
  • Author(s): 平井涼太・星野洋一郎
  • Organizer: 園芸学会平成28年度秋季大会
  • Place of Presentation: 名城大学天白キャンパス（愛知県名古屋市）
  • Year and Date: 2016-09-11
• [Presentation] フローサイトメトリーとAFLP マーカーを用いたユーラシアのハスカップの系統解析 (2015)
  • Author(s): 松本彩・Artem Sorokin・星野洋一郎
  • Organizer: 園芸学会
  • Place of Presentation: 千葉大学（千葉県千葉市）
  • Year and Date: 2015-03-28 – 2015-03-29
• [Presentation] 中国および日本のハスカップ倍数性レベル、DNA含量変異およびAFLPによる多様性解析 (2013)
  • Author(s): 石山知美・下田真明・小野寺康之・青木宣明・叶玉紅・雷 俊仏・孔 徳剛・翁 海竜・星野洋一郎
  • Organizer: 園芸学会 : 園芸学研究 第12巻別冊1号(2013)
  • Place of Presentation: 東京農工大学小金井キャンパス(東京都)