Exploration of functional-metal nano particle-producing bacteria from extremophile environments

• Project/Area Number: 24405030
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 海外学術
• Research Field: Applied biochemistry
• Research Institution: Kyoto University
• Principal Investigator: Kawamoto Jun 京都大学, 化学研究所, 助教 (90511238)
• Co-Investigator(Kenkyū-buntansha): KURIHARA Tastuo 京都大学, 化学研究所, 教授 (70243087)
• Project Period (FY): 2012-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥17,290,000 (Direct Cost: ¥13,300,000、Indirect Cost: ¥3,990,000); Fiscal Year 2014: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000); Fiscal Year 2013: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2012: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
• Keywords: 金属ナノ粒子 / 極限環境微生物 / 低温菌 / 菌体外膜小胞 / 特殊環境微生物 / 金属呼吸 / 微生物機能

Outline of Final Research Achievements

Metal nanoparticles have significantly different physicochemical characteristics from bulk metal materials, and their catalytic activities have great interests in various fields. In this study, to develop a synthesis method for metal nanoparticles at moderate environments, we attempted to explore metal nanoparticle-producing bacteria and their synthesis mechanism. Pseudomonas sp. H206 isolated from inner Mongolia and Shewanella livingstonensis Ac10 isolated from Antarctic seawater, which produce silver nanoparticles at the extracellular region, secret vesicles surrounded by lipid bilayer, and the size of membrane vesicles are about 100 nm. To elucidate the vesiculation mechanism of S. livingstonensis Ac10, we performed molecular characterization of the membrane vesicles of this strain. Moreover, the production of uniformly sized spherical vesicles might be involved in the synthesis of nano sized metal particle by these microorganisms.

Research Products

• [Presentation] 低温菌 Shewanella livingstonensis Ac10 の金属呼吸に関与する外膜輸送タンパク質の解析 (2014)
  • Author(s): 丸山沙織、川本 純、栗原 達夫
  • Organizer: 第１５回極限環境生物学会
  • Place of Presentation: 沖縄
  • Year and Date: 2014-11-02
• [Presentation] Shewanella livingstonensis Ac10 の金属呼吸における外膜タンパク質 PhoE の機能 (2014)
  • Author(s): 丸山沙織、樽井 淳、王 玉、川本 純、栗原 達夫
  • Organizer: 日本農芸化学会 2014年度大会
  • Place of Presentation: 明治大学生田キャンパス
• [Presentation] Shewanella livingstonensis Ac10における金属還元メカニズムの解析 (2013)
  • Author(s): 丸山沙織、樽井 淳、王 玉、川本 純、栗原 達夫
  • Organizer: 第14回極限環境生物学会年会
  • Place of Presentation: 明治大学生田キャンパス