| Budget Amount *help |
¥17,290,000 (Direct Cost: ¥13,300,000、Indirect Cost: ¥3,990,000)
Fiscal Year 2014: ¥5,460,000 (Direct Cost: ¥4,200,000、Indirect Cost: ¥1,260,000)
Fiscal Year 2013: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000)
Fiscal Year 2012: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
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| Outline of Final Research Achievements |
Metal nanoparticles have significantly different physicochemical characteristics from bulk metal materials, and their catalytic activities have great interests in various fields. In this study, to develop a synthesis method for metal nanoparticles at moderate environments, we attempted to explore metal nanoparticle-producing bacteria and their synthesis mechanism. Pseudomonas sp. H206 isolated from inner Mongolia and Shewanella livingstonensis Ac10 isolated from Antarctic seawater, which produce silver nanoparticles at the extracellular region, secret vesicles surrounded by lipid bilayer, and the size of membrane vesicles are about 100 nm. To elucidate the vesiculation mechanism of S. livingstonensis Ac10, we performed molecular characterization of the membrane vesicles of this strain. Moreover, the production of uniformly sized spherical vesicles might be involved in the synthesis of nano sized metal particle by these microorganisms.
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