Serological and molecular epidemiological studies on Asian zoonotic schistosomiasis in the Philippines

• Project/Area Number: 24405044
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 海外学術
• Research Field: Applied veterinary science
• Research Institution: Obihiro University of Agriculture and Veterinary Medicine
• Principal Investigator: KAWAZU Shin-ichiro 帯広畜産大学, 原虫病研究センター, 教授 (60312295)
• Co-Investigator(Kenkyū-buntansha): KIRINOKI Masashi 獨協医科大学, 医学部, 准教授 (50265302)
• Co-Investigator(Renkei-kenkyūsha): KADOHIRA Mutsuyo 帯広畜産大学, 畜産学部, 教授 (20313976)
• Project Period (FY): 2012-04-01 – 2016-03-31
• Project Status: Completed (Fiscal Year 2015)
• Budget Amount: ¥17,420,000 (Direct Cost: ¥13,400,000、Indirect Cost: ¥4,020,000); Fiscal Year 2014: ¥5,330,000 (Direct Cost: ¥4,100,000、Indirect Cost: ¥1,230,000); Fiscal Year 2013: ¥5,720,000 (Direct Cost: ¥4,400,000、Indirect Cost: ¥1,320,000); Fiscal Year 2012: ¥6,370,000 (Direct Cost: ¥4,900,000、Indirect Cost: ¥1,470,000)
• Keywords: 人獣共通感染症 / 日本住血吸虫症 / フィリピン / 保虫宿主 / 中間宿主貝 / スイギュウ

Outline of Final Research Achievements

Serological and molecular epidemiological studies for the reservoir host animals on Asian zoonotic schistosomiasis were conducted in the Philippines. In addition, preliminary analysis on genetic diversity of Schistosoma japonicum in the study sites using microsatellite markers was also done. (1) The field study suggested that water buffaloes and dogs are the major animal reservoirs contributing to human transmission of S. japonicum in Central Visayas and Southern Mindanao regions in the Philippines. (2) The study also indicated the importance of utilizing water buffaloes as sentinel animals for schistosomiasis surveillance. (3) The analytical protocol for multi-locus genotyping (MLG) was established using 10 microsatellite markers. (4) Preliminary analysis among parasite populations from 4 study sites revealed that there are no particular spatial structuring among these parasite populations. This finding suggested that there is an on-going gene flow between the populations.

Research Products

• [Int'l Joint Research] フィリピン大学マニラ校公衆衛生学部(フィリピン)
• [Journal Article] Water buffalo as sentinel animals for schistosomiasis surveillance. (2015)
  • Author(s): Angeles, JM., Leonardo, L., Goto, Y., Kirinoki, M., Villacorte, E., Hakimi, H., Moendeg, KJ., Seungyeon Lee., Rivera, PT., Inoue, N., Chigusa, Y., and Kawazu, S.
  • Journal Title: Bulletin of the World Health Organization Volume: 93(7) Issue: 7 Pages: 511-512
  • DOI: 10.2471/blt.14.143065
  • Peer Reviewed / Open Access / Int'l Joint Research
• [Journal Article] Development and optimization of cocktail-ELISA for a unified surveillance of zoonotic schistosomiasis in multiple host species. (2015)
  • Author(s): Moendeg, KJ., Angeles, JM., Goto, Y., Leonardo, LR., Kirinoki, M., Villacorte, EA., Rivera, PT., Inoue, N., Chigusa, Y., and Kawazu, S.
  • Journal Title: Parasitology Research Volume: 144 Issue: 3 Pages: 1225-1228
  • DOI: 10.1007/s00436-015-4312-7
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Recent advances in the diagnosis and control of Schistosoma japonicum infection in animals. (2013)
  • Author(s): Angeles, JM., and Kawazu, S.
  • Journal Title: 獣医寄生虫学雑誌 Volume: 12 Pages: 44-50
• [Journal Article] Localization and expression profiling of a 31 kDa antigenic repetitive protein Sjp_0110390 in Schistosoma japonicum life stages (2013)
  • Author(s): Angeles JM, Kirinoki M, Goto Y, Asada M, Hakimi H, Leonardo LR, Tongol-Rivera P, Villacorte EA, Inoue N, Chigusa Y, Kawazu SI
  • Journal Title: Mol Biochem Parasitol Volume: 187 Issue: 2 Pages: 98-102
  • DOI: 10.1016/j.molbiopara.2012.12.002
  • Peer Reviewed
• [Journal Article] Utilization of ELISA Using Thioredoxin Peroxidase-1 and Tandem Repeat Proteins for Diagnosis of Schistosoma japonicum Infection among Water Buffaloes (2012)
  • Author(s): Angeles JM, Goto Y, Kirinoki M, Asada M,Leonardo LR, Rivera PT, Villacorte EA, Inoue N, Chigusa Y, Kawazu S
  • Journal Title: PLoS Negl Trop Dis Volume: 6 Issue: 8 Pages: e1800-e1800
  • DOI: 10.1371/journal.pntd.0001800
  • Peer Reviewed
• [Presentation] Serological comparison of recombinant antigens for the diagnosis of human schistosomiasis. (2016)
  • Author(s): Angeles JMA., Goto Y., Leonardo L., Moendeg KJ., Rivera PT., Villacorte E., Kirinoki M., Inoue N., Chigusa Y., and Kawazu S.
  • Organizer: 第85回日本寄生虫学会
  • Place of Presentation: 宮崎市民プラザ（宮崎県・宮崎市）
  • Year and Date: 2016-03-19
• [Presentation] Epidemiological study of zoonotic schistosomiasis among water buffaloes in different endemic areas in the Philippines. (2015)
  • Author(s): Angeles JMA., Leonardo L., Goto Y., Kirinoki M., Moendeg KJ.,Villacorte E., Inoue N., Chigusa Y., and Kawazu S.
  • Organizer: American Society of Tropical Medicine and Hygiene 64th Annual Meeting 第64回米国熱帯医学会年会
  • Place of Presentation: Marriot Downtown Philadelphia（フィラデルフィア・米国）
  • Year and Date: 2015-10-25
  • Int'l Joint Research
• [Presentation] Utilization of cocktail-antigen ELISA for the detection of zoonotic schistosomiasis in multiple host species. (2015)
  • Author(s): Moendeg KJ., Angeles JMA., Leonardo L., Goto Y., Kirinoki M., Villacorte E., Rivera PT., Inoue N., Chigusa Y., and Kawazu S.
  • Organizer: American Society of Tropical Medicine and Hygiene 64th Annual Meeting 第64回米国熱帯医学会年会
  • Place of Presentation: Marriot Downtown Philadelphia（フィラデルフィア・米国）
  • Year and Date: 2015-10-25
  • Int'l Joint Research
• [Presentation] Use of cocktail-antigen ELISA for the diagnosis of zoonotic schistosomiasis in multiple host species. (2015)
  • Author(s): Moendeg KJ、Angeles JMA、後藤康之、Leonardo L、桐木雅史、Villacorte E、Tongol-Rivera P、井上昇、千種雄一、河津信一郎
  • Organizer: 第61回日本寄生虫学会・日本衛生動物学会 北日本支部合同大会
  • Place of Presentation: 帯広畜産大学・原虫病研究センター（北海道・帯広市）
  • Year and Date: 2015-10-10
• [Presentation] Epidemiological study of zoonotic schistosomiasis among water buffaloes in different endemic areas in the Philippines. (2015)
  • Author(s): Angeles JM., Leonardo L.,Goto Y., Kirinoki M., Moendeg KJ., Villacorte E., Rivera PT., Inoue N., Chigusa Y., and Kawazu S.
  • Organizer: 9thEuropean Congress on Tropical Medicine and International Health 第9回欧州熱帯医学・国際保健会議
  • Place of Presentation: The Congress Center Basel（バーゼル・スイス）
  • Year and Date: 2015-09-06
  • Int'l Joint Research
• [Presentation] Epidemiological study of zoonotic schistosomiasis among water buffaloes in different endemic areas in the Philippines. (2015)
  • Author(s): Angeles JM., Leonardo L.,Goto Y., Kirinoki M., Moendeg KJ., Villacorte E., Rivera PT., Inoue N., Chigusa Y., and Kawazu S.
  • Organizer: 5thAnnual Workshop of the Regional Network on Asian Schistosomiasis and Other Helminth Zoonoses 第5回アジア寄生虫ワークショップ会議（RNAS+）
  • Place of Presentation: The Bellevue Alabang（モンテンルパ・フィリピン）
  • Year and Date: 2015-07-23
  • Int'l Joint Research
• [Presentation] Animal surveillance in zoonotic schistosomiasis: a prevalence and experiential study in the Philippines. (2015)
  • Author(s): Angeles JM., Leonardo L.,Goto Y., Kirinoki M., Moendeg KJ., Rivera PT., Villacorte E., Inoue N., Chigusa Y., and Kawazu S.
  • Organizer: 第84回日本寄生虫学会
  • Place of Presentation: 杏林大学・三鷹キャンパス（東京都・三鷹市）
  • Year and Date: 2015-03-21 – 2015-03-22
• [Presentation] Water buffaloes as sentinel animals for the surveillance of zoonotic schistosomiasis. (2014)
  • Author(s): Angeles JMA., Leonardo L., Goto Y., Kirinoki M., Hakimi H., Moendeg KJ., Tongol-Rivera P., Villacorte E., Inoue N., Chigusa Y., and Kawazu S.
  • Organizer: 第63回米国熱帯医学会年会
  • Place of Presentation: Marriot/Sheraton New Orleans（ニューオリンズ・米国）
  • Year and Date: 2014-11-02 – 2014-11-06
• [Presentation] Water buffaloes as sentinel animal population for zoonotic schistosomiasis surveillance. (2014)
  • Author(s): Angeles JM、Leonardo L、後藤康之、桐木雅史、Villacorte E、Hakimi H、Moendeg K、Lee S、Tongol-Rivera P、井上昇、千種雄一、河津信一郎
  • Organizer: 第157回日本獣医学会学術集会
  • Place of Presentation: 北海道大学・高等教育推進機構（北海道・札幌市）
  • Year and Date: 2014-09-09 – 2014-09-12
• [Presentation] Water buffaloes as sentinel animal population in the zoonotic schistosomiasis surveillance and control. (2014)
  • Author(s): Angeles JM, Leonardo LR, Goto Y, Kirinoki M, Hakimi H, Moendeg KJ, Rivera PT, Villacorte EA, Inoue N, Chigusa Y and Kawazu S.
  • Organizer: 第13回国際寄生虫会議（ICOPA XIII）
  • Place of Presentation: Camino Real Hotel（メキシコシティ・メキシコ）
  • Year and Date: 2014-08-10 – 2014-08-15
• [Presentation] Detection of canine schistosomiasis japonica using recombinant thioredoxin peroxidase-1 and tandem repeat proteins. (2014)
  • Author(s): Angeles JM., Goto Y., Kirinoki M., Leonardo L., Boldero NP., Hakimi H., Lee S., Rivera PT., Villacorte E., Inoue N., Chigusa Y., and Kawazu S.
  • Organizer: 第83回日本寄生虫学会
  • Place of Presentation: 愛媛大学・城北キャンパス（愛媛県・松山市）
  • Year and Date: 2014-03-27 – 2014-03-28
• [Presentation] Development of serological techniques for the diagnosis of zoonotic Schistosoma japonicum infection through the use of recombinant proteins. (2013)
  • Author(s): Angeles JMA., Goto Y., Kirinoki M., Leonardo L., Asada M., Hakimi H., Villacorte E., Tongol-Rivera P., Inoue N., Chigusa Y., and Kawazu S.
  • Organizer: 第62回米国熱帯医学会年会
  • Place of Presentation: Marriot Wardman Park Washington（ワシントンDC・米国）
  • Year and Date: 2013-11-13 – 2013-11-17
• [Presentation] Detection of canine schistosomiasis japonica using recombinant thioredoxin peroxidase-1 and tandem repeat proteins. (2013)
  • Author(s): Angeles JM., Goto Y., Leonardo L., Kirinoki M., Hakimi H., Rivera PT., Villacorte E., Inoue N., Chigusa Y., and Kawazu S.
  • Organizer: 第54回日本熱帯医学会大会
  • Place of Presentation: 長崎ブリックホール（長崎県・長崎市）
  • Year and Date: 2013-10-04 – 2013-10-05
• [Presentation] Development of recombinant antigen-based serological diagnosis for the detection of Schistosoma japonicum infection in water buffaloes. (2013)
  • Author(s): Angeles JM., Goto Y.,Kirinoki M., Leonardo L., Asada M., Rivera PT., Villacorte E., Inoue N., Chigusa Y., and Kawazu S.
  • Organizer: 第24回世界獣医寄生虫学会議（WAAVP）
  • Place of Presentation: Perth Convention Exhibition Centre Western Australia（パース・オーストラリア）
  • Year and Date: 2013-08-25 – 2013-08-29
• [Presentation] Localization and expression profiling of a 31 kDa antigenic repetitive protein Sjp 0110390 in Schistosoma japonicum life stages. (2013)
  • Author(s): Angeles, JM., et al.
  • Organizer: 第82回日本寄生虫学会
  • Place of Presentation: 東京医科歯科大学 湯島キャンパス
  • Year and Date: 2013-03-30
• [Presentation] Development of recombinant antigen-based serological diagnosis for the detection of Schistosoma japonicum infection in water buffaloes. (2013)
  • Author(s): Angeles, JM., et al.
  • Organizer: 第155回日本獣医学会学術集会
  • Place of Presentation: 東京大学 駒場キャンパス(招待講演)
  • Year and Date: 2013-03-28
• [Presentation] Utilization of ELISA using Thioredoxin peroxidase-1 and tandem repeat proteins for diagnosis of Schistosoma japonicum infection among water buffaloes. (2012)
  • Author(s): Angeles, JM., et al.
  • Organizer: American Society of Tropical Medicine and Hygiene 61^<th> Annual Meeting 第61回米国熱帯医学会年会
  • Place of Presentation: Atlanta Marriot Marquis (アトランタ、米国)
  • Year and Date: 2012-11-13
• [Presentation] Evaluation of recombinant proteins for Schistosoma japonicum infection diagnosis among water buffaloes in two new endemic foci in the Philippines. (2012)
  • Author(s): Angeles, JM., et al.
  • Organizer: 第53回日本熱帯医学会大会
  • Place of Presentation: 帯広市とかちプラザ
  • Year and Date: 2012-09-06
• [Presentation] Evaluation of recombinant thioredoxin peroxidase-1 and tandem repeat proteins as antigens for serodiagnosis of Schistosoma japonicum infections. (2012)
  • Author(s): Angeles, JM., et al.
  • Organizer: 5^<th> ASEAN Congress of Tropical Medicine and Parasitology 第5回ASEAN熱帯医学寄生虫学会議
  • Place of Presentation: Citystate Tower Hotel, Manila (マニラ、フィリピン共和国)
  • Year and Date: 2012-05-18
• [Book] Schistosomiasis: Epidemiology, Diagnosis and Treatment. (ed., A. Miele), Chapter 5, Insights into animal schistosomiasis: From surveillance to control. (2014)
  • Author(s): Angeles, JM., and Kawazu, S.
  • Total Pages: 196
  • Publisher: Nova Publishing Inc., New York
• [Remarks] 帯広畜産大学・原虫病研究センター
  • URL: http://www.obihiro.ac.jp/~protozoa/index.html
• [Remarks] 原虫病研究センター・先端予防治療学分野
  • URL: http://www.obihiro.ac.jp/~tryp/index.html
• [Remarks] 帯広畜産大学・原虫病研究センター河津信一郎
  • URL: http://www.obihiro.ac.jp/~protozoa/index.html
• [Remarks]
  • URL: http://www.obihiro.ac.jp/~tryp/index.html