Development of the quick diagnostic method for identifying Taenia asiatica and Taenia saginata for food safety and analysis of the gene causing the host specificity.

• Project/Area Number: 24406011
• Research Category: Grant-in-Aid for Scientific Research (B)
• Allocation Type: Partial Multi-year Fund
• Section: 海外学術
• Research Field: Parasitology (including Sanitary zoology)
• Research Institution: Kyoto University
• Principal Investigator: OKAMOTO Munehiro 京都大学, 霊長類研究所, 教授 (70177096)
• Co-Investigator(Kenkyū-buntansha): SAKO Yasuhito 旭川医科大学, 医学部, 教授 (40312459) ; YANAGIDA Tetsuya 山口大学, 共同獣医学部, 准教授 (40431837)
• Co-Investigator(Renkei-kenkyūsha): OKAMOTO Yoshiharu 鳥取大学, 農学部, 教授 (50154410) ; MATSUI Atsushi 京都大学, 霊長類研究所, 研究員 (70455476)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥17,290,000 (Direct Cost: ¥13,300,000、Indirect Cost: ¥3,990,000); Fiscal Year 2014: ¥4,940,000 (Direct Cost: ¥3,800,000、Indirect Cost: ¥1,140,000); Fiscal Year 2013: ¥5,850,000 (Direct Cost: ¥4,500,000、Indirect Cost: ¥1,350,000); Fiscal Year 2012: ¥6,500,000 (Direct Cost: ¥5,000,000、Indirect Cost: ¥1,500,000)
• Keywords: テニア条虫 / 交雑 / 無鉤条虫 / アジア条虫 / 中間宿主 / 家畜 / テニア状虫

Outline of Final Research Achievements

Taenia tapeworms collected from humans were examined for the mitochondrial cox1 gene and two nuclear genes, ef1, elp and pold. Phylogenetic analysis revealed that Taenia saginata and Taenia asiatica were once separated into two distinct taxa. On the other hand, some worms showed nuclear-mitochondrial discordance. Those facts indicate that they originated from hybridization between T. saginata and T. asiatica.

Research Products

• [Journal Article] The present situation and towards the prevention and control of neurocysticercosis on the tropical island, Bali, Indonesia. (2015)
  • Author(s): Wandra T, Swastika IK, Dharmawan NS, Purba IE, Sudamaja IM, Yoshida T, Sako Y, Okamoto M, Diarthini NLPE, Laksemi DAAS, Yanagida T, Nakao M, Ito A.
  • Journal Title: Parasites and Vectors Volume: 8 Issue: 1 Pages: 148-148
  • DOI: 10.1186/s13071-015-0755-z
  • NAID: 120005758428
  • Peer Reviewed / Open Access / Acknowledgement Compliant
• [Journal Article] Phylogenetic characterisation of Taenia tapeworms in spotted hyenas and reconsideration of the “Out of Africa” hypothesis of Taenia in humans. (2014)
  • Author(s): Terefe Y, Hailemariam Z, Menkir S, Nakao M, Lavikainen A, Haukisalmi V, Iwaki T, Okamoto M, Ito A.
  • Journal Title: International Journal of Parasitology Volume: 44 Issue: 8 Pages: 533-541
  • DOI: 10.1016/j.ijpara.2014.03.013
  • Peer Reviewed / Acknowledgement Compliant
• [Journal Article] Molecular identification of species of Taenia causing bovine cysticercosis in Ethiopia. (2013)
  • Author(s): Hailemariam Z, Nakao M, Menkir S, Lavikainen A, Iwaki T, Yanagida T, Okamoto M, Ito A.(8)
  • Journal Title: Journal of Helminthology Volume: 4 Issue: 3 Pages: 1-5
  • DOI: 10.1017/s0022149x13000138
  • Peer Reviewed / Open Access
• [Journal Article] Molecular phylogeny of the genus Taenia (Cestoda: Taeniidae): Proposals for the resurrection of Hydatigera Lamarck, 1816 and the creation of a new genus Versteria (2013)
  • Author(s): Nakao M, Lavikainen A, Iwaki T, Haukisalmi V, Konyaev S, Oku Y, Okamoto M, Ito A.
  • Journal Title: International Journal for Parasitology Volume: 43(6) Issue: 6 Pages: 427-37
  • DOI: 10.1016/j.ijpara.2012.11.014
  • Peer Reviewed
• [Journal Article] Mini review on chemotherapy of taeniasis and cysticercosis due to Taenia solium in Asia, and a case report with 20 tapeworms in China (2013)
  • Author(s): Ito A, Li T, Chen X, Long C, Yanagida T, Nakao M, Sako Y, Okamoto M, Wu Y, Raoul F, Giraudoux P, Craig PS.
  • Journal Title: Tropical Biomedicine Volume: 30 Pages: 164-173
  • NAID: 120005553881
• [Journal Article] Echinococcus multilocularis: Single hepatic lesion experimentally established without metastasis in rats. (2013)
  • Author(s): Yamashita M, Imagawa T, Nakaya K, Sako Y, Okamoto Y, Tsuka T, Osaki T, Okamoto M, Ito A.
  • Journal Title: Experimental Parasitology Volume: 135 Issue: 2 Pages: 320-324
  • DOI: 10.1016/j.exppara.2013.07.015
  • Peer Reviewed
• [Journal Article] Advances in diagnosis and spatial analysis of cysticercosis and taeniasis. (2013)
  • Author(s): Raoul F, Li T, Sako Y, Chen X, Long C, Yanagida T, Wu Y, Nakao M, Okamoto M, Craig PS, Giraudoux P, Ito A.
  • Journal Title: Parasitology Volume: 140 Issue: 13 Pages: 1578-1588
  • DOI: 10.1017/s0031182013001303
  • Peer Reviewed
• [Journal Article] Simple and reliable preparation of immunodiagnostic antigens for Taenia solium cysticercosis. (2013)
  • Author(s): Sako Y, Itoh S, Okamoto M, Nakaya K, Ito A.
  • Journal Title: Parasitology Volume: 140 Issue: 13 Pages: 1589-1594
  • DOI: 10.1017/s0031182013000978
  • NAID: 120006517752
  • Peer Reviewed
• [Journal Article] Genotypic relationships between Taenia saginata and Taenia asiatica and their hybrids. (2013)
  • Author(s): Yamane K, Yanagida T, Li T, Chen X, Dekumyoy P, Waikagul J, Nkouawa A, Nakao M, Sako Y, Ito A, Sato H, Okamoto M.
  • Journal Title: Parasitology Volume: 140 Issue: 13 Pages: 1595-1601
  • DOI: 10.1017/s0031182013001273
  • Peer Reviewed
• [Journal Article] Detection of human taeniases in Tibetan endemic areas, China. (2013)
  • Author(s): Li T, Chen X, Yanagida T, Wang H, Long C, Sako Y, Okamoto M, Wu Y, Giraudoux P, Raoul F, Nkouawa A, Nakao M, Craig PS, Ito A.
  • Journal Title: Parasitology Volume: 140 Issue: 13 Pages: 1602-1607
  • DOI: 10.1017/s003118201300111x
  • Peer Reviewed
• [Journal Article] Taeniasis and cysticercosis in Indonesia:past and present situation. (2013)
  • Author(s): Wandra T, Ito A, Swastika K, Dharmawan NS, Sako Y, Okamoto M.
  • Journal Title: Parasitology Volume: 140 Issue: 13 Pages: 1608-1616
  • DOI: 10.1017/s0031182013000863
  • Peer Reviewed
• [Journal Article] Genetic polymorphisms of Echinococcus granulosus in the Middle East (2012)
  • Author(s): Yanagida T, Mohammadzadeh T, Kamhawi S, Nakao M, Sadjjadi SM, Hijjawi N, Abdel-Hafez SK, Sako Y, Okamoto M, Ito A
  • Journal Title: Parasitol Int Volume: 61 Issue: 4 Pages: 599-603
  • DOI: 10.1016/j.parint.2012.05.014
  • Peer Reviewed
• [Journal Article] A Loop-mediated isothermal amplification method for a differential identification of Taenia tapeworms from human: application to a field survey (2012)
  • Author(s): Nkouawa A, Sako Y, Li T, Chen X, Nakao M, Yanagida T, Okamoto M, Giraudoux P, Raoul F, Nakaya K, Xiao N, Qiu J, Qiu D, Craig PS, Ito A.
  • Journal Title: Parasitol Int Volume: 61 Issue: 4 Pages: 723-725
  • DOI: 10.1016/j.parint.2012.06.001
  • Peer Reviewed
• [Journal Article] Usefulness of pumpkin seeds combined with areca nut extract in community-based treatment of human taeniasis in northwest Sichuan province (2012)
  • Author(s): Li T, Ito A, Chen X, Long C, Okamoto M, Raoul F, Giraudoux P, Yanagida T, Nakao M, Xiao N,Craig PS
  • Journal Title: Acta Trop Volume: 124 Issue: 2 Pages: 152-157
  • DOI: 10.1016/j.actatropica.2012.08.002
  • Peer Reviewed
• [Presentation] The species problem of Taenia asiatica and differentiation of three human taeniid species (2014)
  • Author(s): Munehiro Okamoto
  • Organizer: JITMM2014
  • Place of Presentation: BangKok, Thailand
  • Year and Date: 2014-12-02 – 2014-12-04
  • Invited
• [Presentation] Genetic relationship between Taenia asiatica and Taenia saginata. (2014)
  • Author(s): Munehiro Okamoto
  • Organizer: Strengthening of Prevention and Control Program of Taeniasis/Neurocysticercosis (T/NCC) in Bali, Indonesia
  • Place of Presentation: Udayana Univ. Bali, Indonesia
  • Year and Date: 2014-09-22
  • Invited
• [Presentation] アジア条虫Taenia asiaticaと無鉤条虫Taenia saginataの遺伝的関係 (2014)
  • Author(s): 岡本宗裕、山根香菜子、中尾稔、柳田哲矢、迫康仁、伊藤亮
  • Organizer: 第157回日本獣医学会学術集会
  • Place of Presentation: 北海道大学
  • Year and Date: 2014-09-09 – 2014-09-12
• [Presentation] Chaotic situation of taeniases and cysticercosis as neglected tropical or zoonotic diseases in Asia. (2014)
  • Author(s): Akira Ito1 and Munehiro Okamoto2 and the working group in Asia
  • Organizer: 6th ASEAN Congress of Tropical Medicine and Parasitology
  • Place of Presentation: Kuala Lumpur, Malaysia
  • Invited
• [Presentation] Molecular re evaluation of Taenia asiatica. (2012)
  • Author(s): Munehiro Okamoto
  • Organizer: Joint International Tropical Medicine Meeting 2012.
  • Place of Presentation: Centra Grand & Bangkok Convention Center at Central World, Bangkok, Thailand
  • Year and Date: 2012-12-13
• [Presentation] Joint Projects on taeniasis and cysticercosis in Thailand. (2012)
  • Author(s): Dekumyoy P, Kusolsuk T, Pakdee W, Sa-nguankiat S, Chaisri K, Homsuwan N, Yanagida T, Sako Y, Nakao M, Okamoto M, Ito A.
  • Organizer: Joint International Tropical Medicine Meeting 2012.
  • Place of Presentation: Centra Grand & Bangkok Convention Center at Central World, Bangkok, Thailand(招待講演)
  • Year and Date: 2012-12-13
• [Presentation] What is Taenia asiatica? (2012)
  • Author(s): Okamoto M, Li T, Chen X, Anantaphruti MT, Waikagul J, Yanagida T, Nakao M, Sako Y, Ito A.
  • Organizer: International Symposium of Cestode Zoonoses Control.
  • Place of Presentation: Paradice Hotel, Shanghai, China(招待講演)
  • Year and Date: 2012-10-29
• [Presentation] Taeniasis/cys ticercosis in farmer communities of Western Sichuan, China : a spatial study. (2012)
  • Author(s): Raoul F, Giraudoux P, Li T, Yanagida T, Lon C, Chen X, Okamoto M, Nakao M, Sako Y, Ito A.
  • Organizer: Internat ional Symposium of Cestode Zoonoses Control.
  • Place of Presentation: Paradice Hotel, Shanghai, China
  • Year and Date: 2012-10-29
• [Presentation] Taeniasis/cysticercosis in Karagasem, Bali, Indonesia. International Symposium of Cestode Zoonoses Control. (2012)
  • Author(s): Swastica K, Wandra T, Sudarmaj a M, Dharmawan NS, Laksemi DS, Diarthini LPE, Yanagida T, Sako Y, Okamoto M, Ito A
  • Organizer: Internat ional Symposium of Cestode Zoonoses Control.
  • Place of Presentation: Paradice Hotel, Shanghai, China
  • Year and Date: 2012-10-29
• [Presentation] Overview of the present situation of taeniasis/cyst icercosis in Indonesia. (2012)
  • Author(s): Wandra T, Swastica K, Dharmawan NS, Sako Y, Okamoto M, Ito A.
  • Organizer: International Symposium of Cestode Zoonoses Control.
  • Place of Presentation: Paradice Hotel, Shanghai, China
  • Year and Date: 2012-10-29
• [Presentation] Pig cysticercos is in Karagasem, Bali, Indonesia. International Symposium of Cestode Zoonoses Control. (2012)
  • Author(s): Dharmawan NS, Swastica K, Suardita IK, Kepeng IN, Sako Y, Okamoto M, Yanagida T, Wandra T, Ito A.
  • Organizer: International Symposium of Cestode Zoonoses Control.
  • Place of Presentation: Paradice Hotel, Shanghai, China
  • Year and Date: 2012-10-29
• [Presentation] Taeniasis and Cysticercosis on the Thai-Myanmer border : an update. (2012)
  • Author(s): Dekumyoy P, Kusolsuk T, Sa-nguanki at S, Chaisiri K, Homsuwan N, Yanagida T, Sako Y, Nakao M, Okamoto M, Ito A.
  • Organizer: International Symposium of Cestode Zoonoses Control.
  • Place of Presentation: Paradice Hotel, Shanghai, China
  • Year and Date: 2012-10-29
• [Presentation] Usefulness of pumpkin seeds combined with areca nut extract in community-based treatment of human taeniasis in Northwest Sichuan Province, China. (2012)
  • Author(s): i, T, Ito A, Chen X, Lon C, Okamoto M, Raoul F, Giradoux P, Yanagida T, Nakao M, Sako Y, Xiao N, Craig P.
  • Organizer: Intenational Symposium of Cestode Zoonoses Control.
  • Place of Presentation: Paradice Hotel, Shanghai, China
  • Year and Date: 2012-10-29
• [Presentation] Outbreak of Taenia asiatica infection in Japan. (2012)
  • Author(s): Yamasak i H, Muto M, Morishima Y Susiyama H Okamoto M.
  • Organizer: International Symposium of Cestode Zoonoses Control.
  • Place of Presentation: Paradice Hotel, Shanghai, China
  • Year and Date: 2012-10-29
• [Book] Taenia in Molecular Detection of Human Parasitic Pathogens, Section III Platyhelminthes : Cestoda, Chapter 27. (2012)
  • Author(s): Munehiro Okamoto and Akira Ito
  • Total Pages: 871
  • Publisher: CRC Press, Boca Raton