Elucidation of the molecular mechanism underlying Drosophila olfactory memory by the mutant analysis based on gene expression profiles

• Project/Area Number: 24500376
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Neuroscience in general
• Research Institution: The University of Tokyo
• Principal Investigator: MURAKAMI Satoshi 東京大学, 分子細胞生物学研究所, 助教 (10463902)
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥4,680,000 (Direct Cost: ¥3,600,000、Indirect Cost: ¥1,080,000); Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2013: ¥1,820,000 (Direct Cost: ¥1,400,000、Indirect Cost: ¥420,000); Fiscal Year 2012: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
• Keywords: 記憶 / 嗅覚 / ショウジョウバエ / 遺伝子 / 嗅覚記憶 / small GTPase

Outline of Final Research Achievements

Based on the previously collated data of gene expressions in a small region of Drosophila brain that is responsible for the olfactory memory, I identified rgk1 as a novel memory gene. rgk1 expresses specifically in the mushroom body (MB) and is required in the MB for the maintenance of inter-mediatememory (IMM). rgk1 encodes a small-GTPase protein homologous to mammalian REM family proteins and was reported to regulate neural activities through the regulation of voltage-gated calcium channels. miRNA-dependent knockdown or a deletion of the rgk1 gene resulted in decreased IMM. Rgk1 function was localized in the MB and forced-expression of rgk1 in the MB resulted in memory enhancement. Antibody analysis of Rgk1 expression within the single MB neurons revealed that Rgk1 localizes to synapses and artificial retrieval of synaptic Rgk1 led to a failure in maintaining olfactory memory after cold-shock treatment.

Research Products

• [Journal Article] The NAV2 homolog Sickie regulates F-actin-mediated axonal growth in Drosophila mushroom body neurons via the non-canonical Rac-Cofilin pathway (2014)
  • Author(s): Abe, T., Yamazaki, D., Murakami, S., Hiroi, M., Nitta, Y., Maeyama, Y., and Tabata T.
  • Journal Title: Development Volume: 141 Issue: 24 Pages: 4716-4728
  • DOI: 10.1242/dev.113308
  • Peer Reviewed / Acknowledgement Compliant
• [Presentation] A mushroom-body-specific small GTPase Rgk1 regulates the anesthesia-resistant component of Drosophila olfactory memory (2014)
  • Author(s): Satoshi Murakami, Maki Minami, Ryuichiro Nakato, Katsuhiko Shirahige, Tetsuya Tabata
  • Organizer: 第37回日本神経科学大会
  • Place of Presentation: パシフィコ横浜
  • Year and Date: 2014-09-11 – 2014-09-13
• [Presentation] A mushroom-body-specific small GTPase Rgk1 regulates the anesthesia-resistant component of Drosophila olfactory memory (2014)
  • Author(s): Satoshi Murakami, Maki Minami, Ryuichiro Nakato, Katsuhiko Shirahige, Tetsuya Tabata
  • Organizer: 新学術領域「多様性から明らかにする記憶ダイナミズムの共通原理」、班会議
  • Place of Presentation: シェラトンホテル札幌
  • Year and Date: 2014-06-16 – 2014-06-18
• [Presentation] The requirement of a mushroom-body-specific RGK family protein in Drosophila olfactory memory retention (2013)
  • Author(s): Satoshi Murakami
  • Organizer: 平成25年度『包括型脳科学研究推進支援ネットワーク』 夏のワークショップ
  • Place of Presentation: 名古屋国際会議場
• [Presentation] Drosophila olfactory memory (2013)
  • Author(s): Tetsuya Tabata
  • Organizer: 平成２５年度新学術領域研究「多様性から明らかにする記憶ダイナミズムの共通原理」班会議
  • Place of Presentation: 京都ガーデンパレス
• [Presentation] The requirement of a mushroom-body-specific RGK family protein in Drosophila olfactory memory retention (2013)
  • Author(s): Satoshi Murakami
  • Organizer: 第36回日本分子生物学会年会
  • Place of Presentation: 神戸ポートアイランド
• [Presentation] ショウジョウバエ嗅覚記憶回路の機能解析 (2012)
  • Author(s): 多羽田哲也、村上智史、山崎大介、廣井誠、阿部崇志、新田陽平、上岡雄太郎
  • Organizer: 新学術領域研究「神経系の動作原理を明らかにするためのシステム分子行動学」班会議
  • Place of Presentation: メルパルク仙台
• [Presentation] ショウジョウバエ嗅覚記憶回路の機能解析 (2012)
  • Author(s): 多羽田哲也、村上智史、山崎大介、廣井誠、阿部崇志、新田陽平、上岡雄太郎
  • Organizer: 包括脳夏のワークショップにおける公開班会議
  • Place of Presentation: 仙台国際センター
• [Presentation] sickie はアクチン脱重合制御因子との相互作用によってショウジョウ バエ嗅覚記憶中枢キノコ体の神経軸索の伸長を制御する (2012)
  • Author(s): 阿部崇志 山崎大介 前山有子 村上智史 多羽田哲也
  • Organizer: 包括脳ネットワーク夏のワークショップ
  • Place of Presentation: 仙台国際センター
• [Presentation] キノコ体における転写産物解析を通じた嗅覚忌避記憶メカニズムの探索 (2012)
  • Author(s): 村上智史
  • Organizer: 第五回分子高次機能研究会ー高次機能を生み出すエコプロセッサ
  • Place of Presentation: KKRホテル琵琶湖