Studies on an ultraviolet-absorbing photopigment, Opsin 5 in vertebrate eye and brain
| Project/Area Number |
24500382
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| Research Category |
Grant-in-Aid for Scientific Research (C)
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| Allocation Type | Multi-year Fund |
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| Section | 一般 |
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| Research Field |
Neuroscience in general
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| Research Institution | Okayama University |
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Principal Investigator |
OHUCHI Hideyo 岡山大学, 医歯(薬)学総合研究科, 教授 (00253229)
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| Co-Investigator(Renkei-kenkyūsha) |
BANDO Tetsuya 岡山大学, 大学院医歯薬学総合研究科, 助教 (60423422)
FUJITA Hirofumi 岡山大学, 大学院医歯薬学総合研究科, 助教 (20423288)
YASUE Akihiro 徳島大学, 大学院HBS研究部, 講師 (80380046)
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| Research Collaborator |
INOUE Junji
KATO Mutsuko
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| Project Period (FY) |
2012-04-01 – 2015-03-31
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| Project Status |
Completed (Fiscal Year 2014)
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| Budget Amount *help |
¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000)
Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000)
Fiscal Year 2012: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
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| Keywords | オプシン５ / Opsin 5 / Opn5 / 光 / 紫外光 / 眼 / 脳 / 視床下部 / 神経内分泌 / opsin 5 / 網膜 / 光受容 / 視物質 / GPCR / 発生分化 |
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| Outline of Final Research Achievements |
This research project was aimed for clarifying the functions for an ultraviolet-absorbing photopigment, Opsin 5 in vertebrates such as mouse and chicken by use of a genome-editing technique, TALEN (Transcription activator-like effector nuclease). We succeeded in production of Opn5-knockout mice by this method, finding that Opn5-deficient mice were born alive and seemed healthy at least after 3 weeks postnatal.
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Report
(4 results)
Research Products
(24 results)
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[Journal Article] Evolution of mammalian Opn5 as a specialized UV-absorbing pigment by a single amino acid mutation.2014
Author(s)
Yamashita T*, Ono K*, Ohuchi H*, Yumoto A, Gotoh H, Tomonari S, Sakai K, Fujita H, Imamoto Y, Noji S, Nakamura K, Shichida Y. (*equally contributed)
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Journal Title
J Biol Chem
Volume: 289
Pages: 3991-4000
NAID
Related Report
Peer Reviewed
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[Journal Article] Lhx1 in the proximal region of the optic vesicle permits neural retina development in the chicken2012
Author(s)
Kawaue, T., Okamoto, M., Matsuyo, A., Inoue, J., Ueda, Y., Tomonari, S., Noji, S., Ohuchi, H
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Journal Title
Biology Open
Volume: 1
Issue: 11
Pages: 1083-1093
DOI
Related Report
Peer Reviewed
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[Journal Article] Non-transgenic genome modifications in a hemimetabolous insect using zinc-finger and TAL effector nucleases.2012
Author(s)
Watanabe T, Ochiai H, Sakuma T, Horch HW, Hamaguchi N, Nakamura T, Bando T, Ohuchi H, Yamamoto T, Noji S, Mito T.
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Journal Title
Nature Communications
Volume: 3
Issue: 1
Pages: 1017-1017
DOI
Related Report
Peer Reviewed
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[Presentation] Immunohistochemical analysis of opsin 5, an ultraviolet-absorbing photopigment, in chicken and mouse neural tissues2015
Author(s)
Mutsuko Kato, Katsuhiko Ono, Takahiro Yamashita, Satomi Katayama, Keita Sato, Hirofumi Fujita, Tetsuya Bando, Yoichi Kondo, Yoshinori Shichida, Hideyo Ohuchi
Organizer
第120回日本解剖学会（第92回日本生理学会合同大会）
Place of Presentation
神戸市
Year and Date
2015-03-21 – 2015-03-23
Related Report
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[Presentation] Molecular properties of Opn5L1, a photoreceptor protein found in non-mammalian vertebrates2014
Author(s)
Keita Sato, Takahiro Yamashia, Hideyo Ohuchi, Sayuri Tomonari, Atsuko Takeuchi, Sari-Fujita-Yanagibayashi, Kazumi Sakai, Yasushi Iwamoto, Sumihare Noji, Akimori Wada, Yoshinori Shichida
Organizer
16th International Conference on Retinal Proteins-ICRP2014-
Place of Presentation
長浜市
Year and Date
2014-10-05 – 2014-10-10
Related Report
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