Studies on an ultraviolet-absorbing photopigment, Opsin 5 in vertebrate eye and brain

• Project/Area Number: 24500382
• Research Category: Grant-in-Aid for Scientific Research (C)
• Allocation Type: Multi-year Fund
• Section: 一般
• Research Field: Neuroscience in general
• Research Institution: Okayama University
• Principal Investigator: OHUCHI Hideyo 岡山大学, 医歯(薬)学総合研究科, 教授 (00253229)
• Co-Investigator(Renkei-kenkyūsha): BANDO Tetsuya 岡山大学, 大学院医歯薬学総合研究科, 助教 (60423422) ; FUJITA Hirofumi 岡山大学, 大学院医歯薬学総合研究科, 助教 (20423288) ; YASUE Akihiro 徳島大学, 大学院HBS研究部, 講師 (80380046)
• Research Collaborator: INOUE Junji ; KATO Mutsuko
• Project Period (FY): 2012-04-01 – 2015-03-31
• Project Status: Completed (Fiscal Year 2014)
• Budget Amount: ¥5,200,000 (Direct Cost: ¥4,000,000、Indirect Cost: ¥1,200,000); Fiscal Year 2014: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2013: ¥1,430,000 (Direct Cost: ¥1,100,000、Indirect Cost: ¥330,000); Fiscal Year 2012: ¥2,340,000 (Direct Cost: ¥1,800,000、Indirect Cost: ¥540,000)
• Keywords: オプシン５ / Opsin 5 / Opn5 / 光 / 紫外光 / 眼 / 脳 / 視床下部 / 神経内分泌 / opsin 5 / 網膜 / 光受容 / 視物質 / GPCR / 発生分化

Outline of Final Research Achievements

This research project was aimed for clarifying the functions for an ultraviolet-absorbing photopigment, Opsin 5 in vertebrates such as mouse and chicken by use of a genome-editing technique, TALEN (Transcription activator-like effector nuclease). We succeeded in production of Opn5-knockout mice by this method, finding that Opn5-deficient mice were born alive and seemed healthy at least after 3 weeks postnatal.

Research Products

• [Journal Article] Evolution of mammalian Opn5 as a specialized UV-absorbing pigment by a single amino acid mutation. (2014)
  • Author(s): Yamashita T*, Ono K*, Ohuchi H*, Yumoto A, Gotoh H, Tomonari S, Sakai K, Fujita H, Imamoto Y, Noji S, Nakamura K, Shichida Y. (*equally contributed)
  • Journal Title: J Biol Chem Volume: 289 Pages: 3991-4000
  • NAID: 120005690203
  • Peer Reviewed
• [Journal Article] The expression of LIM-homeobox genes, Lhx1 and Lhx5, in the forebrain is essential for neural retina differentiation. (2013)
  • Author(s): Inoue J, Ueda Y, Bando T, Mito T, Noji S, Ohuchi H.
  • Journal Title: Dev Growth Differ Volume: 55 Issue: 7 Pages: 668-675
  • DOI: 10.1111/dgd.12074
  • NAID: 40019813303
  • Peer Reviewed
• [Journal Article] Molecular aspects of eye evolution and development: from the origin of retinal cells to the future of regenerative medicine. (2013)
  • Author(s): Ohuchi H
  • Journal Title: Acta Med Okayama Volume: 67 Pages: 203-312
  • Peer Reviewed
• [Journal Article] Lhx1 in the proximal region of the optic vesicle permits neural retina development in the chicken (2012)
  • Author(s): Kawaue, T., Okamoto, M., Matsuyo, A., Inoue, J., Ueda, Y., Tomonari, S., Noji, S., Ohuchi, H
  • Journal Title: Biology Open Volume: 1 Issue: 11 Pages: 1083-1093
  • DOI: 10.1242/bio.20121396
  • Peer Reviewed
• [Journal Article] Non-transgenic genome modifications in a hemimetabolous insect using zinc-finger and TAL effector nucleases. (2012)
  • Author(s): Watanabe T, Ochiai H, Sakuma T, Horch HW, Hamaguchi N, Nakamura T, Bando T, Ohuchi H, Yamamoto T, Noji S, Mito T.
  • Journal Title: Nature Communications Volume: 3 Issue: 1 Pages: 1017-1017
  • DOI: 10.1038/ncomms2020
  • Peer Reviewed
• [Journal Article] Wakayama symposium: epithelial-mesenchymal interactions in eyelid development. (2012)
  • Author(s): Ohuchi H.
  • Journal Title: Ocul Surf Volume: 10 Issue: 4 Pages: 212-216
  • DOI: 10.1016/j.jtos.2012.07.005
  • Peer Reviewed
• [Presentation] Immunohistochemical analysis of opsin 5, an ultraviolet-absorbing photopigment, in chicken and mouse neural tissues (2015)
  • Author(s): Mutsuko Kato, Katsuhiko Ono, Takahiro Yamashita, Satomi Katayama, Keita Sato, Hirofumi Fujita, Tetsuya Bando, Yoichi Kondo, Yoshinori Shichida, Hideyo Ohuchi
  • Organizer: 第120回日本解剖学会（第92回日本生理学会合同大会）
  • Place of Presentation: 神戸市
  • Year and Date: 2015-03-21 – 2015-03-23
• [Presentation] Comprehensive expression pattern analysis of a tumor suppressor gene, REIC/Dkk3 in the mouse (2015)
  • Author(s): Junji Inoue, Hirofumi Fujita, Tetsuya Bando, Yoichi Kondo, Hiromi Kumon, Hideyo Ohuchi
  • Organizer: 第120回日本解剖学会（第92回日本生理学会合同大会）
  • Place of Presentation: 神戸市
  • Year and Date: 2015-03-21 – 2015-03-23
• [Presentation] CRISPR/CasシステムによるPax6遺伝子破壊マウスの解析 (2014)
  • Author(s): 河野仁美、泰江章博、石丸善康、井上順治、渡辺崇人、板東哲哉、親泊政一、野地澄晴、三戸太郎、田中栄二、大内淑代
  • Organizer: 第37回日本分子生物学会年会
  • Place of Presentation: 横浜市
  • Year and Date: 2014-11-25 – 2014-11-27
• [Presentation] Molecular properties of Opn5L1, a photoreceptor protein found in non-mammalian vertebrates (2014)
  • Author(s): Keita Sato, Takahiro Yamashia, Hideyo Ohuchi, Sayuri Tomonari, Atsuko Takeuchi, Sari-Fujita-Yanagibayashi, Kazumi Sakai, Yasushi Iwamoto, Sumihare Noji, Akimori Wada, Yoshinori Shichida
  • Organizer: 16th International Conference on Retinal Proteins-ICRP2014-
  • Place of Presentation: 長浜市
  • Year and Date: 2014-10-05 – 2014-10-10
• [Presentation] Studies of Opsin 3-related proteins implicate the presence of novel photoreceptors in the chicken retina (2014)
  • Author(s): Hideyo Ohuchi, Takashi Sugiyama, Hirofumi Fujita, Mutsuko Katoh, Hitomi Kohno, Tetsuya Bando
  • Organizer: 第55回日本組織細胞化学会総会・学術集会
  • Place of Presentation: 松本市
  • Year and Date: 2014-09-27 – 2014-09-28
• [Presentation] 非哺乳類脊椎動物が持つ光受容タンパク質Opn5L1の光不活性化 (2014)
  • Author(s): 佐藤恵太，山下高廣，大内淑代，友成さゆり，湯本茜，柳林彩理，酒井佳寿美，野地澄晴，七田芳則
  • Organizer: 日本動物学会第85回大会
  • Place of Presentation: 仙台市
  • Year and Date: 2014-09-11 – 2014-09-13
• [Presentation] コオロギにおけるエピジェネティック因子E（z）の概日リズムおよび再生への関与 (2014)
  • Author(s): 濱田良真，板東哲哉，大内淑代，富岡憲治
  • Organizer: 日本動物学会第85回大会
  • Place of Presentation: 仙台市
  • Year and Date: 2014-09-11 – 2014-09-13
• [Presentation] Localization of the two opsin 3-related proteins implicates the presence of novel photoreceptors in the chicken retina (2014)
  • Author(s): Ohuchi H, Fujita H, Inoue J, Bando, T.
  • Organizer: 第119回日本解剖学会総会・全国学術総会
  • Place of Presentation: 栃木県下野市
• [Presentation] 可視光感受性タンパク質Opn5n の分子特性解析 (2013)
  • Author(s): 山下高廣，大内淑代，湯本茜，佐藤恵太， 友成さゆり，木下政人，野地澄晴，七田芳則
  • Organizer: 日本動物学会第84回大会
  • Place of Presentation: 岡山市
• [Presentation] 細胞の多様性を決めるしくみー眼の再生医療に向けてー (2013)
  • Author(s): 大内淑代
  • Organizer: 日本実験動物技術者協会第47回総会
  • Place of Presentation: 倉敷市
  • Invited
• [Presentation] 新規オプシン５様視物質(cOpn5n)のニワトリ網膜における発現様式 (2013)
  • Author(s): 大内淑代、山下高廣、湯本茜、佐藤恵太、井上順治、友成さゆり、野地澄晴、七田芳則
  • Organizer: 第54回日本組織化学会総会・学術集会
  • Place of Presentation: 東京都
• [Presentation] A non-mammalian type opsin 5 functions dually in the photoreceptive and non-photoreceptive organs of birds (2012)
  • Author(s): Hideyo Ohuchi, Takahiro Yamashita, Sayuri Tomonari, Sari Fujita-Yanagibayashi, Kazumi Sakai, Sumihare Noji, Yoshinori Shichida
  • Organizer: 第14回国際組織細胞化学会議 (ICHC2012)
  • Place of Presentation: 京都
• [Presentation] 非哺乳類タイプのオプシン５はニワトリ光受容器官と非光受容器官の両方に存在する (2012)
  • Author(s): 大内淑代、山下高廣、友成さゆり、藤田（柳林）彩理、酒井佳寿美、野地澄晴、七田芳則
  • Organizer: 第35回日本神経科学大会
  • Place of Presentation: 名古屋
• [Presentation] 脊椎動物非視覚オプシンOpn5とその類似光受容タンパク質の分子的性質の解析 (2012)
  • Author(s): Takahiro Yamashita, Hideyo Ohuchi, Sayuri Tomonari, Sari Fujita-Yanagibayashi, Kazumi Sakai, Sumihare Noji, Yoshinori Shichida
  • Organizer: 第50回日本生物物理学会年会
  • Place of Presentation: 名古屋
• [Presentation] Molecular properties of vertebrate non-visual opsins (2012)
  • Author(s): Takahiro Yamashita, Hideyo Ohuchi, Sayuri Tomonari, Sari Fujita-Yanagibayashi, Kazumi Sakai, Sumihare Noji, Yoshinori Shichida
  • Organizer: 15th international Conference on Retinal Proteins
  • Place of Presentation: Ascona, Switzerland
• [Book] 「脳内光受容体」光と生命の事典（真嶋哲朗、飯野盛利、七田芳則、藤堂剛編集、分担執筆） (2016)
  • Author(s): 大内淑代
  • Total Pages: 450
  • Publisher: 朝倉書店
• [Book] 実験医学別冊 今すぐ始めるゲノム編集（山本卓編、分担執筆） (2014)
  • Author(s): 渡辺崇人、三戸太郎、大内淑代、野地澄晴
  • Total Pages: 10
  • Publisher: 羊土社
• [Book] ルイス・ウオルパート著「発生生物学 (サイエンス・パレット) 生物はどのように形づくられるか」 (2013)
  • Author(s): 大内淑代、野地澄晴（訳）
  • Total Pages: 181
  • Publisher: 丸善出版